Recombinant mouse il 6

Fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD11b mAb, Allophycocyanin (APC)-conjugated anti-mouse Gr-1 mAb, Allophycocyanin (APC)-conjugated anti-mouse F4/80 mAb, P-phycoerythrin (PE)-conjugated anti-mouse Ly6G mAb, and Allophycocyanin (APC)-conjugated anti-mouse Ly6C mAb were purchased from Biolegend, and β-Tubulin (2144), p-S6 (4858S), S6 (2217S), p-4EBP-1 (2855S), and 4EBP-1 (9644 T) were from cell Signal Technology Inc. mTOR inhibitor INK128 was purchased from Selleckchem. Trizol Reagent and SYBR green dye were bought from Invitrogen. Recombinant mouse IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), and MDSC Isolation Kit were obtained from Miltenyi Biotec. Glucose and streptozotocin (STZ) were purchased from Sigma-Aldrich.

INK128, rapamycin, and metformin were purchased from Selleckchem Inc. Recombinant mouse IFN-α2, anti-CD11b-fluorescein isothiocyanate (FITC), anti-Gr1-allophycocyanin (APC), anti-Ly6G-phycoerythrin (PE), and anti-Ly6C-APC were purchased from Biolegend Inc. TRIzol reagent and SYBR green dye were purchased from Invitrogen Inc. Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco Inc. Collagenase type D and DNase I were purchased from Roche Inc. Pristane, N-methyl-2-pyrrolidone (NMP), and polyvinyl pyrrolidone (PVP) were purchased from Sigma Inc. Antibodies for α-tubulin (2144), p-S6 (4858S), S6 (2217S), p-4EBP-1 (2855S), 4EBP-1 (9644T), p-mTOR (5536T), p-AMPK (25375), and IRF-8 (83413T) were purchased from Signal Technology Inc. MDSC isolation kit, recombinant mouse IL-6, and GM-CSF were purchased from Miltenyi Biotec Inc. R848, CpG, poly I:C, and TNF-ɑ were purchased from Enzo Life Science Inc. Mouse albumin enzyme linked immunosorbent assay (ELISA) quantitation set, mouse anti-IgG, and anti-dsDNA IgG kit were purchased from Bethyl Laboratories Inc.