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The KMS-28BM is a laboratory equipment designed for cell culture applications. It is a cell culture incubator that maintains optimal temperature, humidity, and carbon dioxide levels to support the growth and maintenance of various cell types.

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Lab products found in correlation

Kms 20, by Japanese Collection of Research Bioresources Cell Bank (4 mentions) Mm 1s, by American Type Culture Collection (3 mentions) Kms12bm, by Japanese Collection of Research Bioresources Cell Bank (3 mentions) Rpmi8226, by American Type Culture Collection (3 mentions) Kms 21 bm, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Nci h929, by American Type Culture Collection (2 mentions) Kms 26, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kms 11, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Rpmi 1640 medium, by PAN Biotech (1 mentions) Fetal bovine serum (fbs), by Lonza (1 mentions) Rpmi 1640 medium, by American Type Culture Collection (1 mentions) L glutamine, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Rpmi 1640, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Streptomycin, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Kms 12 pe, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Nci h929, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)

6 protocols using kms 28bm

1

Multiple Myeloma Cell Culture

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The U-266, RPMI-8226, IM-9 and MM.1S cells were obtained from American Type Culture Collection (ATCC, USA). The KMS-28BM, KMS-12BM and KMS-20 were purchased from the Japanese Collection of Research Bioresources (JCRB) cell bank. Multiple myeloma cells were cultured and maintained in RPMI-1640 medium (PAN-Biotech, Germany) with 10% fetal bovine serum (Sigma-Aldrich, Germany) in an incubator at 37 ℃ with 5% CO2. Cells were sub-cultured when achieved 70–75% confluence. Cells at the logarithmic phase were used for transfection.
Bong I.P., Ng C.C., Othman N, & Esa E. (2022). Gene expression profiling and in vitro functional studies reveal RAD54L as a potential therapeutic target in multiple myeloma. Genes & Genomics, 44(8), 957-966.
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2

Myeloma Cell Line Culture Protocol

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Eight myeloma cell lines were used in this study. The RPMI-8226, U-266, MM.1S, and IM-9 MM cells were purchased from American Type Culture Collection (ATCC, USA). Myeloma cell lines KMS-28-BM, KMS-20, KMS-12-BM, and KMS-21-BM were obtained from Japanese Collection of Research Bioresources (JCRB) cell bank. Cells were cultured with RPMI1640 medium (ATCC) supplemented with 10–15% fetal bovine serum (Lonza) in an incubator at 37 °C with humidified 5% CO2. Cells were passaged every 3–4 days.
Bong I.P., Ng C.C., Baharuddin P, & Zakaria Z. (2017). MicroRNA expression patterns and target prediction in multiple myeloma development and malignancy. Genes & Genomics, 39(5), 533-540.
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3

Multiple Myeloma Cell Lines Protocol

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KMS-11, KMS-21BM, KMS-26, and KMS-28BM were obtained from JCRB Cell Bank, NCI-H929 and U266B1 were from ATCC (Manassas, Virginia, USA); and OPM2 and LP-1 were from DSMZ (Braunschweig, Germany). Cells were cultured as described in the supplier’s instructions.
Tomita U., Ishimoto Y., Ri M., Kawase Y., Hizukuri Y., Maru C., Nanai K., Nakamura R., Nakayama M., Oguchi-Oshima K., Sumi H., Ohtsuka T., Iida S, & Agatsuma T. (2024). A novel T cell-redirecting anti-GPRC5D × CD3 bispecific antibody with potent antitumor activity in multiple myeloma preclinical models. Scientific Reports, 14, 5135.
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4

Evaluating BTZ and TIG Synergy in MM Cell Lines

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Cell lines KMS20 (JCRB1196) and KMS28BM (JCRB1192) were obtained from the Japanese Collection of Research Bioresources Cell Bank (Ibaraki city, Osaka, Japan).
Cells were grown in suspension at 37 °C and 5% CO2 with RPMI-1640 supplemented with 10% (v/v) of FBS; 2 µmol/L L-glutamine; 100 U/mL penicillin G; and 100 µg/mL streptomycin (all of them from Gibco, ThermoFisher Scientific, Waltham, MA, USA). Cells were cultured in 6-well plates and exposed to vehicle dimethylsulfoxide (DMSO), BTZ, TIG, or a constant dose of BTZ (12.5 nM for KMS20 and 4.5 nM for KMS28BM) with increasing TIG concentrations (5–60 µM). BTZ and TIG were obtained from Selleck Chemicals (Houston, TX, USA) and dissolved in DMSO to a stock concentration of 100 mmol/L.
Ramos-Acosta C., Huerta-Pantoja L., Salazar-Hidalgo M.E., Mayol E., Jiménez-Vega S., García-Peña P., Jordi-Cruz J., Baquero C., Porras A., Íñigo-Rodríguez B., Benavente C.M., López-Pastor A.R., Gómez-Delgado I., Urcelay E., Candel F.J, & Anguita E. (2024). Tigecycline Opposes Bortezomib Effect on Myeloma Cells Decreasing Mitochondrial Reactive Oxygen Species Production. International Journal of Molecular Sciences, 25(9), 4887.
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5

Culturing Multiple Myeloma Cell Lines

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The human MM cell lines U266, MM.1S, RPMI8226, and NCI-H929 were purchased from the American Type Culture Collection (Manassas, VA, USA). The KMS-11, KMS-12-BM, KMS-12-PE, KMS-28-BM, and KMS-28-PE cell lines were purchased from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan). All MM cell lines except NCI-H929 were cultured in RPMI1640 (GIBCO, Thermo Fisher, Grand Island, NY, USA) containing 10% fetal bovine serum (GIBCO, Thermo Fisher) and 1% penicillin/streptomycin (P/S) (GIBCO, Thermo Fisher).
Lee K.J., Lee K.H., Yoon K.A., Sohn J.Y., Lee E., Lee H., Eom H.S, & Kong S.Y. (2017). Chromothripsis in Treatment Resistance in Multiple Myeloma. Genomics & Informatics, 15(3), 87-97.
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6

Cell Culture Maintenance Protocol

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KMS-20, KMS-26, and KMS-28BM cells were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). These cells were maintained in RPMI 1640 medium (Sigma, St. Louis, MO, USA) supplemented with 25 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (pH 7.4; FUJIFILM Wako), 100 U/mL streptomycin (Gibco, Carlsbad, CA, USA), 100 μg/mL penicillin (Gibco), and 10% fetal bovine serum (Gibco) in an atmosphere of 5% CO2.
Tsubaki M., Takeda T., Matsuda T., Seki S., Tomonari Y., Koizumi S., Nagatakiya M., Katsuyama M., Yamamoto Y., Tsurushima K., Ishizaka T, & Nishida S. (2021). Activation of Serum/Glucocorticoid Regulated Kinase 1/Nuclear Factor-κB Pathway Are Correlated with Low Sensitivity to Bortezomib and Ixazomib in Resistant Multiple Myeloma Cells. Biomedicines, 9(1), 33.
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