RPMI-1640 medium, FBS, penicillin (100 U/mL), and streptomycin sulfate (100 (μg/mL) are from Life Technologies. The MTT, dimethyl sulfoxide (DMSO), RNase A, leupeptin, aprotinin, phenylmethylsulfonylfluoride (PMSF), dithiothreitol, and Triton X-100 were purchased from Sigma-Aldrich Co. CREB-binding protein (CBP), PTGS2, CFLAR, BCL2, BCL2L1, NF-κB p65 subunit, PARP, and β-actin antibodies were purchased from Santa Cruz Biotechnology, Inc. XIAP antibody was purchased from BD Biosciences. RSF1 and hSNF2H antibodies were purchased from Upstate. The RNA Extraction Kit was purchased from Intron Biotechnology. The Luciferase Assay Kit was purchased from Promega. pNF-κB–Luc reporter plasmid was purchased from BD Biosciences. Lipofectamine was purchased from Invitrogen. RSF1, PTGS2, CFLAR, XIAP, BCL2, BCL2L1, and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) oligonucleotide primers were purchased from Bioneer Technology. Paclitaxel was purchased from A.G. Scientific, Inc. The IκB inhibitor Bay 11-7082 and the proteasome inhibitor MG132 were obtained from Calbiochem. PureProteome protein A magnetic beads were purchased from Millipore.
Xiap antibody
Manufactured by BD
Sourced in United States
The XIAP antibody is a laboratory tool used for research purposes. It is designed to detect and bind to the X-linked inhibitor of apoptosis protein (XIAP), which is a key regulator of cell death pathways. The antibody can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and distribution of XIAP in biological samples. The core function of the XIAP antibody is to facilitate the identification and analysis of XIAP in a research setting.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Santa Cruz Biotechnology (3 mentions)
Caspase 9, by Cell Signaling Technology (3 mentions)
Bcl xl, by Cell Signaling Technology (3 mentions)
Bcl 2, by Cell Signaling Technology (3 mentions)
Cytochrome c, by Santa Cruz Biotechnology (3 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)
Bcl 2, by Santa Cruz Biotechnology (2 mentions)
Annexin 5, by Thermo Fisher Scientific (2 mentions)
P akt, by Cell Signaling Technology (2 mentions)
Caspase 3, by Cell Signaling Technology (2 mentions)
Embelin, by Bio-Techne (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Luciferase assay kit, by Promega (1 mentions)
Pureproteome protein a magnetic beads, by Merck Group (1 mentions)
Streptomycin sulfate, by Thermo Fisher Scientific (1 mentions)
Bcl2l1, by Santa Cruz Biotechnology (1 mentions)
β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Nf κb p65 subunit, by Santa Cruz Biotechnology (1 mentions)
Lipofectamine, by Thermo Fisher Scientific (1 mentions)
5 protocols using xiap antibody
1
Comprehensive Cellular Evaluation Protocol
2
Inhibition of HSP90 in B-cell Malignancies
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A high-affinity HSP90-inhibitor, AUY922 [30 (link)] was purchased from Selleckchem. Antibodies to HSP90αβ, PLCγ2, BCAP, CD19, AXL, BCL2, GAPDH and actin were purchased from Santa Cruz Biotechnologies. Antibodies to CD79a, CD79b, LYN, SYK, BTK, AKT, P-ERK1/2, ERK1/2, STAT3, PTPN22, FGFR3, and MCL-1 were purchased from Cell Signaling Technologies. XIAP antibody, chromogen-conjugated antibodies to human CD5 and CD19 or fluorescein isothiocyanate (FITC)-conjugated annexin V were obtained from BD Biosciences or Invitrogen, respectively. Propidium iodide (PI) and other chemicals were purchased from Sigma or Bio-Rad. Replication-deficient lentiviral constructs expressing HSP90-specific shRNA or GFP tagged control scrambled shRNA were purchased from Santa Cruz Biotechnologies.
3
Thiostrepton Inhibits FoxM1 Signaling
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Thiostrepton (FoxM1 selective inhibitor) was purchased from Tocris Cookson Inc (Ellisville, MO). Antibodies against caspase-3, caspase-9, p-AKT, Cox IV, β-actin, MMP-2, MMP-9, Bcl-2, BID, Bcl-xL and poly (ADP) ribose polymerase (PARP) antibodies were purchased from Cell Signaling Technologies (Beverly, MA). FoxM1, VEGF, Cytochrome c and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). XIAP antibody and trans-well invasion and migration kits were purchased from BD Biosciences (San Jose, CA). Annexin V and JC1 were purchased from Thermo Fisher Scientific (Waltham, MA). Live dead assay kit was purchased from Life Technologies (Grand Island, NY).
4
Olaparib and Embelin Cytotoxicity Assay
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Olaparib was purchased from Selleck Chemicals (Houston, TX). Embelin was purchased from Tocris Bioscience (Ellisville, MO). Antibodies against PARP, caspase-8, caspase-9, caspase-3, Bcl-2, Bcl-xl and cIAP1 were purchased from Cell Signaling Technologies (Beverly, MA). XIAP antibody was purchased from BD Pharmingen (San Diego, CA, USA). Survivin, Cytochrome c and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Annexin V was purchased from Thermo Fischer Scientific (Waltham, MA). caspase-8 inhibitor, z-IETD-FMK was purchased from R&D Systems (Minneapolis, MN, USA).
5
Molecular Pathways of Embelin-Induced Apoptosis
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Embelin was purchased from Tocris Bioscience (Ellisville, MO). MTT was purchased from Sigma (St. Louis MO, MA). LY294002 and zVAD-fmk was purchased from Calbiochem (San Diego, CA, USA). XIAP antibody was purchased from BD Transduction lab (San Jose, CA, USA). Antibodies against caspase-9, caspase-3, PARP, p-AKT, p-Bad, Bcl-2, Bcl-Xl, Beta-actin, Survivin and Bid were purchased from Cell Signaling Technologies (Beverly, MA, USA). Cytochrome c and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). cIAP-1 antibody was purchased from R&D (USA). Annexin V/PI staining kit was purchased from Molecular Probes (Eugene OR, USA).
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