Api 4000

Manufactured by AB Sciex

Sourced in United States, Japan, Canada, Germany, United Kingdom, France

The API 4000 is a triple quadrupole mass spectrometer designed for high-throughput quantitative analysis. It features a robust atmospheric pressure ionization (API) source, multiple scan modes, and high sensitivity detection capabilities.

Automatically generated - may contain errors

Lab products found in correlation

Analyst 1, by AB Sciex (11 mentions) Absoluteidq p180 kit, by Biocrates (5 mentions) Lc 20ad, by Shimadzu (4 mentions) Luna c18 column, by Phenomenex (4 mentions) Cbm 20a lc, by Shimadzu (4 mentions) Lc 20ad system, by Shimadzu (3 mentions) Sil htc, by Shimadzu (3 mentions) Api 2000, by AB Sciex (3 mentions) Qtrap 5500, by AB Sciex (3 mentions) Analyst software, by AB Sciex (3 mentions) 1100 hplc system, by Agilent Technologies (3 mentions) Kinetex hilic column, by Phenomenex (3 mentions) Uptidisc ptfe filters, by Interchim (3 mentions) 1200 series, by Agilent Technologies (2 mentions) Waters acquity uplc, by Waters Corporation (2 mentions) 4 hydroxyproline, by Avantor (2 mentions) Sequant zic hilic, by Merck Group (2 mentions) Metidq software, by Biocrates (2 mentions) Agilent 1200 series, by Agilent Technologies (2 mentions) Male ob ob mice, by Janvier Labs (2 mentions)

203 protocols using api 4000

Quantification of Dotinurad and Oxaprozin in Plasma and Urine

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Plasma concentrations of dotinurad and oxaprozin were determined by a validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method (LC: LC-20AD system for dotinurad, SHIMADZU; LC-10ADvp, CTO-10ASvp, DGU-14A, and SIL-HTc for oxaprozin, SHIMADZU. MS/MS: API4000, SCIEX). The lower limit of quantification (LLOQ) was 1 ng/mL for dotinurad and 1 μg/mL for oxaprozin. Ultrafiltered plasma samples were used to determine unbound plasma concentrations of dotinurad by a validated LC–MS/MS method (LC:LC-20AD system, SHIMADZU. MS/MS: API4000, SCIEX). The LLOQ was 0.1 ng/mL.
Urinary concentrations of metabolites (glucuronate conjugates and sulfate conjugates) were determined by a validated LC-MS/MS method (LC: Nexera X2 and Prominence, SHIMADZU. MS/MS: Triple Quad 4500, SCIEX). The LLOQ was 10 ng/mL for both metabolites.

Furihata K., Nagasawa K., Hagino A, & Kumagai Y. (2020). A drug–drug interaction study of a novel, selective urate reabsorption inhibitor dotinurad and the non-steroidal anti-inflammatory drug oxaprozin in healthy adult males. Clinical and Experimental Nephrology, 24(Suppl 1), 36-43.

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Quantification of Dotinurad and Metabolites

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Plasma levels of dotinurad were determined by liquid chromatography tandem mass spectrometry (LC–MS/MS) method (LC: LC-20AD system, SHIMADZU. MS/MS: API4000, SCIEX) at Sekisui Medical Co., Ltd. (Tokyo, Japan). The lower limit of quantification (LLOQ) was 1 ng/mL.
Urinary levels of metabolites (glucuronate conjugate and sulfate conjugate) were determined by LC–MS/MS method (LC: Nexera X2, Prominence, SHIMADZU. MS/MS: Triple Quad 4500, SCIEX) at Fuji Yakuhin Co., Ltd. (Saitama, Japan). The LLOQ was 10 ng/mL.
In order to calculate the unbound fraction in plasma, dotinurad was added to baseline plasma samples, which were then ultrafiltered and underwent LC–MS/MS measurement (LC: LC-20AD system, SHIMADZU. MS/MS: API4000, SCIEX) at Sekisui Medical Co., Ltd. The LLOQ was 0.1 ng/mL.

Kumagai Y., Sakaki M., Furihata K., Ito T., Inoue K., Yoshida T., Matsumoto S., Furuno K, & Hagino A. (2019). Dotinurad: a clinical pharmacokinetic study of a novel, selective urate reabsorption inhibitor in subjects with hepatic impairment. Clinical and Experimental Nephrology, 24(Suppl 1), 25-35.

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Lemborexant Pharmacokinetics Bioanalysis

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Blood samples (4 mL each) were collected for the assessment of lemborexant PK, with sampling times for each study presented in Supplemental Table 1. Lemborexant concentrations in plasma were determined by liquid‐liquid extraction followed by analyte quantification using reverse‐phase high‐performance liquid chromatography‐mass spectrometry (AB Sciex API 4000, SCIEX, Concord, Ontario, Canada) operated under multiple reaction monitoring‐positive ion mode. The assay monitored mass‐to‐charge ratio (m/z) 411.0 → 287.1 for lemborexant and 414.0 → 290.1 for the deuterated internal standard. The lower limit of quantification of the assay and its linear calibration range were 0.05 and 0.05‐50.0 ng/mL, respectively, with appropriate bioanalytical noninterference of coadministered compounds demonstrated before sample analysis. The validated method had an interday and intraday precision and accuracy (bias) of less than 12%, with incurred sample reanalysis passing the criteria in each individual study. Successful cross‐validation was established across 2 bioanalytical laboratories that handled all sample analyses.

Lalovic B., Majid O., Aluri J., Landry I., Moline M, & Hussein Z. (2020). Population Pharmacokinetics and Exposure‐Response Analyses for the Most Frequent Adverse Events Following Treatment With Lemborexant, an Orexin Receptor Antagonist, in Subjects With Insomnia Disorder. Journal of Clinical Pharmacology, 60(12), 1642-1654.

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Synthesis and Characterization of Z-X Compounds

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The synthesis of Z-X-1 and Z-X-2 compounds is shown in Scheme 1 and Scheme 2 (details are provided in the Supporting Materials). All new compounds were fully characterized by mass spectrometry (MS; AB Sciex API4000, SCIEX, Framingham, MA, USA) and ¹H nuclear magnetic resonance (NMR; INOVA, Agilent, Santa Clara, CA, USA) imaging.

Zhang Z., Chen Z., Li C., Xiao Z., Luo Y., Pan X., Xu L, & Feng X. (2023). Synthesis, Biophysical Properties, and Antitumor Activity of Antisense Oligonucleotides Conjugated with Anisamide. Pharmaceutics, 15(6), 1645.

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Cytarabine Nanoparticle Development and Evaluation

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Cytarabine (Ara-C) was purchased from the Aladdin Industrial Corporation. Palmitic acid (PA), sodium hydroxide, potassium dihydrogen phosphate, sodium dihydrogen phosphate and disodium hydrogen phosphate were bought from Sinopharm Chemical Reagent Co., Ltd. Ethyl chloroformate (EtOCOCl) was obtained from Chengdu Beisite Reagent Co., Ltd. Pepsin (1 : 3000) and trypsin (1 : 250) were purchased from Amresco and Sangon Biotech (Shanghai) Co., Ltd, respectively. Acyclovir (HPLC > 98%) was purchased from Dalian Meilun Biotech Co., Ltd. HL60 and K562 cells were kindly provided by the Immunopharmacology Institute of Shandong University and the Shandong Analysis and Test Center. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from Beijing Solarbio Technology Co., Ltd.
The spectroscopy equipment was: an NMR spectrometer (¹H-NMR, Bruker Avance 400), an electrospray tandem mass spectrometer (MS, AB SCIEX API 4000), an FTIR spectrometer (Nicolet 6700), a transmission electron microscope (TEM, JEM-200CX), a high performance liquid chromatograph (HPLC, Agilent Technologies 1200 Series), an HPLC-MS (Agilent 1260 triple quadrupole mass spectrometer equipped with an ESI source), and a microplate reader (ELISA, PerkinElmer).

Zhang J., Zhang D., Hu X., Liu R., Li Z, & Luan Y. (2018). Rational design of a new cytarabine-based prodrug for highly efficient oral delivery of cytarabine. RSC Advances, 8(24), 13103-13111.

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Quantitative LC-MS/MS Analysis of Lyso-Gb3

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Quantitative LC-MS/MS analysis was performed on an AB SCIEX API 4000 triple quadrupole mass spectrometer (AB Sciex LLC, Framingham, MA, USA) operating in positive mode. An XDB-C18 column (2.1×100 mm, 3.5 µm particles) was used in combination with a VanGuard pre-column of the same material (Agilent Technologies, Inc., Santa Clara, CA, USA). The mobile phases consisted of (A) water and (B) acetonitrile/methyl alcohol (15/85=v/v). Each mobile phase contained 0.1% formic acid and 2 mM ammonium formate. The two mobile phases formed the following gradient: 0–0.25 (50% B) min, 0.25–2.0 min (50–99%, B), 2.0–4.5 min (99%, B), 4.5–4.75 min (99–50% B) and 4.75–8.5 min (50%, B). The MS conditions were as follows: ESI mode was positive, ion spray voltage was 5.5 KV, nebulizer gas and auxiliary gas were 60 psi, curtain gas was 25 psi, the desolvation temperature was 600°C and the flow rate is 0.3 ml/min. The following multiple-reaction monitoring (MRM) transitions were monitored, with a dwell time of 100 ms: 786.5>282.4 (lyso-Gb3) and 490.5>292.3 (IS). Collision energy was 48.6 V and 38 V in the MRM traces of lyso-Gb3 and IS, respectively (Fig. 1).

Ouyang Y., Chen B., Pan X., Wang Z., Ren H., Xu Y., Ni L., Yu X., Yang L, & Chen N. (2018). Clinical significance of plasma globotriaosylsphingosine levels in Chinese patients with Fabry disease. Experimental and Therapeutic Medicine, 15(4), 3733-3742.

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Quantification of Kynurenine Pathway Metabolites

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We quantified KYN, kynurenic acid (KA), xanthurenic acid (XA), anthranilic acid (AA), 3-hydroxykynurenine (3-HK), 3-hydroxyanthranilic acid (3-HAA), riboflavin (vitamin B2) and its cofactors, flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN), pyridoxine (vitamin B6), pyridoxic acid, pyridoxal, pyridoxal 5′-phosphate and pyridoxamine from plasma, using isotope dilution liquid chromatography coupled with tandem mass spectrometry (ABSciex API4000; AB SCIEX, Framingham, MA, USA) based on a modified method by Midttun et al. [50 (link)]. Neuroprotective-to-neurotoxic z-score ratios were calculated to account for the balance between neuroprotective metabolites or neurotoxic metabolites/cofactors: zKA/zHA and zKA/zHK. A similar approach was used in [51 (link)].

Duarte-Guterman P., Richard J.E., Lieblich S.E., Eid R.S., Lamers Y, & Galea L.A. (2023). Cellular and molecular signatures of motherhood in the adult and ageing rat brain. Open Biology, 13(11), 230217.

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Powder Characterization by LC-MS/MS

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After the generated powder sample was fully homogenized using ultrasonic treatment for 5 min, it was dispersed in deionized water. A 0.22 μm organic filter membrane was used for filtration, and an AB SCIEX API4000 type LC-MS/MS System was used for detection. The conditions of the experiment were as follows: The mobile phase was a solution of 0.1 % formic acid. The gradient elution method was used to inject 1 μL of methanol. The column temperature was 35 °C, and the flow rate was adjusted to 0.4 mL/min.

Zhang X., Huang Y., Huang S., Xie W., Huang W., Chen Y., Li Q., Zeng F, & Liu X. (2024). Antisolvent precipitation for the synergistic preparation of ultrafine particles of nobiletin under ultrasonication-homogenization and evaluation of the inhibitory effects of α-glucosidase and porcine pancreatic lipase in vitro. Ultrasonics Sonochemistry, 105, 106865.

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Maternal and Cord Blood Lutein Quantification

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Maternal and venous cord blood plasma samples were sent out to Creative Proteomics (Shirley, NY, USA) for lutein level measurement with ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry (UHPLC-MS/MS) using an ABSCIEX API 4000 tandem mass spectrometer (AB Sciex, Framingham, MA, USA) connected to a Waters Acquity UPLC (Waters, Milford, MA, USA). Each plasma sample (50 µL) was mixed with 150 µL of methanol, centrifuged, and filtered through a 0.22 μm membrane filter. The Waters Acquity UPLC BEH C18 column (2.1 × 100 mm 1.7 μm) coupled with a VanGuard precolumn (2.1 × 5 mm 1.7 μm) was used for liquid chromatography. The mobile phase A was pure water with 0.2% formic acid and the mobile phase B was acetonitrile with 0.1% formic acid. The column temperature was held at 40 °C and the injection volume was 5 μL. The gradient was from 98% B in 10 min, with a flow rate of 0.45 mL/min. The ESI positive mode was used for MS and the conditions were as follows: ion source temperature, 550 °C; CUR, 30 psi; IS, 5000V, and GS1 and GS2, 45 psi. The lutein peak was detected at m/z 568.6 and a substantial fragment was detected at m/z 476.3.

Kadam I., Nebie C., Dalloul M., Hittelman J., Fordjour L., Hoepner L., Futterman I.D., Minkoff H, & Jiang X. (2024). Maternal Lutein Intake during Pregnancies with or without Gestational Diabetes Mellitus and Cognitive Development of Children at 2 Years of Age: A Prospective Observational Study. Nutrients, 16(2), 328.

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Lorcaserin Dose-Dependent Pharmacokinetics

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Eight rats which had previously received lorcaserin during the 28-day treatment phase were allowed to remain on the high-fat diet for 8 weeks to allow adequate washout of drug. After this time, the rats were randomly allocated into 2 groups of 4, one group designated lorcaserin 1 mg/kg SC b.i.d and the other lorcaserin 2 mg/kg SC b.i.d. Rats were dosed to a schedule similar to that adopted for the feeding study, that is, 2× daily, 10 h interval between the daytime doses. On day 1 and day 7, blood was collected from the saphenous vein at timepoints pre-dose, 0.25 h, 0.5 h, 1 h, 4 h, 8 h after each daily dose. This allowed comparison between drug exposure on day 1 and day 7 and provided an estimate of drug exposure over the treatment phase of the study. All rats remained on the high-fat diet for the duration of this study phase.
Bioanalytical analyses were conducted using an AB SCIEX API4000 liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) system (AB SCIEX, Framingham, MA). Lorcaserin was measured based on the methods as previously described (Higgins et al. 2013a (link)).

Higgins G.A., Desnoyer J., Van Niekerk A., Silenieks L.B., Lau W., Thevarkunnel S., Izhakova J., DeLannoy I.A., Fletcher P.J., DeLay J, & Dobson H. (2014). Characterization of the 5-HT2C receptor agonist lorcaserin on efficacy and safety measures in a rat model of diet-induced obesity. Pharmacology Research & Perspectives, 3(1), e00084.

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