Cto 20ac

Manufactured by Shimadzu

Sourced in Japan, Germany, Spain, United States

The CTO-20AC is a column oven from Shimadzu, designed for use in high-performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC) systems. It provides precise temperature control and stability to ensure consistent and reliable chromatographic separations.

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Lab products found in correlation

Cbm 20a, by Shimadzu (35 mentions) Spd m20a, by Shimadzu (29 mentions) Sil 20ac, by Shimadzu (25 mentions) Lc 20ad, by Shimadzu (20 mentions) Sil 30ac, by Shimadzu (16 mentions) Lc 30ad, by Shimadzu (12 mentions) Dgu 20a5, by Shimadzu (12 mentions) Hplc system, by Shimadzu (10 mentions) Spd 20a, by Shimadzu (8 mentions) Dgu 20a5r, by Shimadzu (7 mentions) Sil 20ac ht, by Shimadzu (7 mentions) Lc 10at, by Shimadzu (6 mentions) Lc 20ab, by Shimadzu (6 mentions) Dgu 20a3, by Shimadzu (6 mentions) Lc 20at, by Shimadzu (5 mentions) High performance liquid chromatography (hplc), by Shimadzu (5 mentions) Lc 20ad pump, by Shimadzu (5 mentions) Spd 20a uv vis detector, by Shimadzu (4 mentions) Dgu 20a, by Shimadzu (4 mentions) Rid 10a, by Shimadzu (4 mentions)

80 protocols using cto 20ac

Quantifying Extracellular Metabolites via HPLC

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The extracellular glucose concentration was quantified using high-performance liquid chromatography (HPLC) on LC-20AD, SIL-20ACHT, CTO-20AC, and RID-10A instruments (Shimadzu Corp., Kyoto, Japan) equipped with a ligand-exchange chromatography column (ULTRON AF-HILIC-CD, 4.6 mm × 250 or 150 mm, 5 mm, Shimadzu Corp.). The samples were separated using an 85% acetonitrile aqueous solution. The column temperature was set to 60 °C and the flow rate was adjusted to 0.8 mL/min. Five microliter of each sample was injected.
Extracellular organic acids and phosphate were quantified using HPLC on LC-30AD, SIL-30AC, CTO-20AC, and CDD-10AVP instruments (Shimadzu Corp.) equipped with three tandem ion-exclusion chromatography columns (Shim-pack Fast-OA, 7.8 × 100 mm, 5 mm, Shimadzu Corp.) and a guard column (Shim-pack Fast-OA (G), 4.0 × 10 mm, 5 mm, Shimadzu Corp.). Sample separation was achieved using a 5.0 mM p-toluenesulfonate mobile phase. The column temperature was set at 40 °C and the flow rate was adjusted to 0.8 mL/min. CDD-10AVP was used as the detector for the post-column pH-buffered electrical conductivity. Post-column pH buffering was attained using a pH-buffering solution containing 5.0 mM p-toluenesulfonate, 20 mmol/L Bis–Tris, and 0.1 mmol/L EDTA. Five microliter of each sample was injected.

Soma Y., Tominaga S., Tokito K., Imado Y., Naka K., Hanai T., Takahashi M., Izumi Y, & Bamba T. (2023). Trace impurities in sodium phosphate influences the physiological activity of Escherichia coli in M9 minimal medium. Scientific Reports, 13, 17396.

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Chiral HPLC Analysis of Organic Compounds

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Analytical high-performance liquid chromatography (HPLC) experiments were conducted using a Shimadzu HPLC system, which included a CBM-20A system controller, LC-20AD pump, CTO-20AC column oven, and RID-10A detector. The analysis was conducted with a flow rate of 0.5 mL/min and a column temperature maintained at 25 °C. For the isocratic analysis, a Chiralpak IA column from Daicel was employed, with the mobile phase consisting of n-hexane and acetone in varying percentages. Prior to usage, HPLC-grade solvents (n-hexane, acetone) were degassed. Sample solutions were prepared by dissolving the compounds in the appropriate mobile phase, resulting in a concentration of approximately 0.5 mg/mL, followed by injection into the system. Data acquisition and instrument control were expertly managed through the LabSolutions Lite software, version 5.52.

Garberová M., Potočňák I., Tvrdoňová M., Majirská M., Bago-Pilátová M., Bekešová S., Kováč A., Takáč P., Khiratkar K., Kudličková Z., Elečko J, & Vilková M. (2023). Derivatives Incorporating Acridine, Pyrrole, and Thiazolidine Rings as Promising Antitumor Agents. Molecules, 28(18), 6616.

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Chromatographic Separation and Identification of Toxins

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The chromatographic setup from Shimadzu (Izasa, Barcelona, Spain) included a binary LC-10ADVP pump system, autoinjector SIL-20AC with a refrigerated rack at 6 °C, column oven CTO-20AC, a fluorescence detector RF-10AXL and the system controller CBM-20A. The post-column reaction system was formed by a knitted reaction coil of 1 mL (5 m × 0.50 mm i.d., Supelco, Madrid, Spain) immersed in a water bath at 75 °C and two post-column pumps LC-20AD and LC-6A, respectively, from Shimadzu (Izasa, Barcelona, Spain). The separation and identification of toxins was achieved in a porous graphitic carbon Hypercarb^® column (i.d. 4.6 mm × 100 mm, 5-µm particle size, Part Number 35005-104630) from Thermo (Fisher Scientific, Madrid, Spain), inside the column oven at 20 °C.

Rey V., Botana A.M., Alvarez M., Antelo A, & Botana L.M. (2016). Liquid Chromatography with a Fluorimetric Detection Method for Analysis of Paralytic Shellfish Toxins and Tetrodotoxin Based on a Porous Graphitic Carbon Column. Toxins, 8(7), 196.

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HPLC Fingerprinting of Aloe vera Extracts

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The test sample solutions were prepared by separately dissolving the whole leaf and green rind extracts in distilled water to make a concentration of 1 mg/mL. The samples were then filtered through 0.45 μm membrane filters (EZ-Pak®, France) before loaded to the HPLC system for analysis. The samples were prepared in triplicates. The fingerprints of the whole leaf and green rind extracts of A. vera were determined using Reverse HPLC (UFLC Shimadzu, Japan). The chromatographic system comprised a Shimadzu LC-10AT equipped with a communicator CBM-20A (Tokyo, Japan), degassing unit DGU-20A_5R (USA), an LC-20 AD pump coupled with an SPD-20A UV/VIS detector (Tokyo, Japan). The HPLC separation was performed on a Luna® C18 column (5 μm; 250 × 4.6 mm; Phenomenex, U.S.A.) maintained at 25 °C in a Shimadzu column oven (CTO-20 AC, Tokyo Japan). It proceeded via isocratic elution with a mobile phase system methanol: 1% acetic acid in water (3:7 v/v) with an injection volume of 20 μL at a flow rate of 0.6 mL/min and detection at a wavelength of 254 nm.

Nalimu F., Oloro J., Peter E.L, & Ogwang P.E. (2022). Acute and sub-acute oral toxicity of aqueous whole leaf and green rind extracts of Aloe vera in Wistar rats. BMC Complementary Medicine and Therapies, 22, 16.

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UPLC-DAD-ESI-IT-TOF Metabolite Analysis

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The UPLC-DAD-ESI-IT-TOF analysis of the metabolites was performed using Shimadzu LC-30AD instrument equipped with two LC-30AD pumps, an SIL-30AC autosampler, SPD-M20A, CTO-20AC column oven, and CBM-20A system controller, and coupled to an IT-TOF mass spectrometer with an ESI interface.
The chromatography separations were performed on a Shim-pack HR-ODS column (150 mm × 2.1 mm, 3 μm). The column was eluted with a gradient mobile phase A of water-formic acid (100: 0.1, v/v) and B of acetonitrile, The elution gradient was 0–2 min, 5–10% B, 2–4 min, 10–12% B, 4–7 min, 12–18% B, 7–13 min, 18–27% B, 13–32 min, 27–56% B, 32–37 min, 56–100% B, and 37–47 min 100% B. The flow rate was 0.2 mL/min; the injection volume was 5 μL and the column oven temperature was kept at 30°C.
The tandem mass spectrometry analyses were carried out on an IT-TOF (Shimadzu, Japan) with the full scan over m/z 100–900 (MS¹) and m/z 50–900 (MS² and MS³) in the ion model of negative(NI) and positive(PI). The parameters were as follows: heat block and curved desolvation line temperature, 200°C; nebulizing nitrogen gas flow, 1.5 L/min; interface voltage: (+), 4.5 kv; (−), 3.5 kv; detector voltage, 1.56 kv; relative collision-induced dissociation energy, 50%.

Xiao Y., Hu Z., Yin Z., Zhou Y., Liu T., Zhou X, & Chang D. (2017). Profiling and Distribution of Metabolites of Procyanidin B2 in Mice by UPLC-DAD-ESI-IT-TOF-MSn Technique. Frontiers in Pharmacology, 8, 231.

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Hybrid mass spectrometry analysis of MEQ

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The MEQ and its metabolites in the serum and testes were detected by the hybrid IT/TOF mass spectrometry coupled with a high-performance liquid chromatography system (Shimadzu Corp., Kyoto, Japan). The liquid chromatography system (Shimadzu) was connected with a DGU-20A3 degasser, a photodiode array detector (SPD-M20A), a solvent delivery pump (LC-20AD), an autosampler (SIL-20AC), a communication base module (CBM-20A) and a column oven (CTO-20AC).

Liu Q., Lei Z., Huang A., Lu Q., Wang X., Ahmed S., Awais I, & Yuan Z. (2017). Mechanisms of the Testis Toxicity Induced by Chronic Exposure to Mequindox. Frontiers in Pharmacology, 8, 679.

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Quantification of PA Concentrations by LC-MS/MS

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The determination of PA concentrations after incubation with microsomes or supersomes was performed by LC-MS/MS as described earlier by Kaltner et al. (2019) and Enge et al. (2021) [16 (link),57 (link)]. Briefly, a 50 × 2.1 mm Kinetex 2.6 μm Core-Shell EVO C18 100 Å column (Phenomenex, Aschaffenburg, Germany) protected by a SecurityGuard ULTRA EVO C18 2.1 mm guard column (Phenomenex, Aschaffenburg, Germany) was used for chromatographic separation on a Shimadzu Prominence HPLC device (LC-20AB, SIL-20AC HT, CTO-20AC, CBM-20A, Shimadzu, Duisburg, Germany). Column oven temperature was maintained at 30 °C, the flow rate was consistently hold at 0.4 mL/min and the injection volume was 10 µL. The HPLC system was coupled to an API4000 triple quadrupole MS (Sciex, Darmstadt, Germany) which was operated in positive electro spray ionisation (ESI) mode with the following parameters: ionisation voltage: 2500 V; nebuliser gas: 50 psi; heating gas: 50 psi; curtain gas: 30 psi; temperature: 600 °C; collision gas: level 7. The selected PA analytes were determined in multiple reaction monitoring (MRM) mode and quantified by external calibration standards ranging from 10 to 125 nmol/mL in DMEM. The PA content was always normalized to the content at t = 0 h.

Buchmueller J., Kaltner F., Gottschalk C., Maares M., Braeuning A, & Hessel-Pras S. (2022). Structure-Dependent Toxicokinetics of Selected Pyrrolizidine Alkaloids In Vitro. International Journal of Molecular Sciences, 23(16), 9214.

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Shimadzu HPLC-MS/MS Protocol for Quantification

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A Shimadzu high-performance liquid chromatography (HPLC) apparatus including binary pumps, a degasser, an autosampler, a column oven and a control unit (LC-20AB, SIL-20AC HT, CTO-20AC, CBM-20A, Duisburg, Germany) was used for all measurements. The HPLC was coupled to an API4000 triple quadrupole mass spectrometer (MS) provided by Sciex (Darmstadt, Germany). The MS ion source parameters were set as follows: ESI + ionization voltage, 4.200 V; nebulizer gas, 50 psi; heating gas, 50 psi; curtain gas, 35 psi; temperature, 550 °C; collision gas level, 7. The MS parameters used are summarized in Online Resource 1. Data acquisition and processing were conducted with Sciex Analyst (Version 1.6.2) and MultiQuant software (Version 3.0.1).

Piacenza N., Kaltner F., Maul R., Gareis M., Schwaiger K, & Gottschalk C. (2020). Distribution of T-2 toxin and HT-2 toxin during experimental feeding of yellow mealworm (Tenebrio molitor). Mycotoxin Research, 37(1), 11-21.

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Quantification of Mycotoxins in Food

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The food samples were analyzed to detect the presence of DON, 3-acetylated DON (3-ADON), 15-acetylated DON (15-ADON), AME, AOH, and TeA based on the method that was reported by Ji et al. (2022) [48 (link)]. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to evaluate the concentrations of DON and Alternaria toxins in the samples. We used an AB Sciex 6500 Qtrap tandem mass spectrometer (Redwood City, CA, USA) coupled with a Shimadzu LC-30AD quaternary pump (Tokyo, Japan), SIL-30AC autosampler, and CTO-20AC column oven. All the reagents were HPLC or analytical grade. The standards of DON, 3-ADON, 15-ADON, AME, AOH, and TeA were purchased from Romer labs (Union City, MO, USA).
The limit of detection (LOD) and limit of quantification (LOQ) was 1.0 µg/kg and 3.0 µg/kg, respectively, for DON, 3-ADON, 15-ADON and TeA, whereas 0.3 µg/kg and 1.0 µg/kg, respectively, for AOH and AME.

Ji X., Xiao Y., Lyu W., Li M., Wang W., Tang B., Wang X, & Yang H. (2022). Probabilistic Risk Assessment of Combined Exposure to Deoxynivalenol and Emerging Alternaria Toxins in Cereal-Based Food Products for Infants and Young Children in China. Toxins, 14(8), 509.

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HPLC Assay for Silmitasertib in Biological Samples

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The Shimadzu HPLC system (Kyoto, Japan) consisted of an online degasser (DGU-20A), two pumps (LC-30 AD), an autosampler (SIL-30AC), a controller (CBM-20A), and a column oven (CTO-20AC). The chromatographic separation was performed on a Synergi™ hydro-RP C18 column (75 × 2.0 mm, 4 μm) with column temperature maintained at 30°C. An optimized gradient of mobile phase A: 5 mM ammonium formate (pH 6.5) and mobile phase B: 0.1% formic acid in acetonitrile was used to elute silmitasertib and ISTD. The flow rate was set at 0.4 ml/min. Two types of gradient elution were employed in this assay. For analyzing human plasma and CSF samples, the gradient elution started with 50% mobile phase B, gradually increased to 80% B in 1.2 min, maintained constant for 3 mins, and then decreased to 50% in 0.3 min, followed by column equilibrium for 1.5 min. For the analysis of brain tissue homogenate, the gradient stated with 30% mobile phase B, reached 80% B in 1.2 min and held this composition for 3 mins, and then return to the initial condition in 0.3 min and held it for 1.5 min. The total analysis time for a single sample was 6 min for both gradients.

Zhong B., Campagne O., Salloum R., Purzner T, & Stewart C.F. (2020). LC-MS/MS method for quantitation of the CK2 inhibitor silmitasertib (CX-4945) in human plasma, CSF, and brain tissue, and application to a clinical pharmacokinetic study in children with brain tumors. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1152, 122254.

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