Log In Sign up
The largest database of trusted experimental protocols

510 confocal microscope

Manufactured by Zeiss
Visit Supplier
Sourced in Germany, United States

The Zeiss 510 confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It features a scanning laser source that provides precise, high-resolution imaging capabilities. The microscope is capable of capturing detailed images of various sample types, allowing for in-depth analysis and observation.

Automatically generated - may contain errors

Lab products found in correlation

Apochromat water immersion objectives, by Zeiss (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Ubiquitin lysine 48, by Merck Group (2 mentions) Zscan4, by OriGene (2 mentions) Rnf20, by Cell Signaling Technology (2 mentions) Vectashield, by Vector Laboratories (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Ab97959, by Abcam (2 mentions) Vectashield mounting liquid, by Vector Laboratories (2 mentions) Rneasy mini kit, by Qiagen (2 mentions) Ssoadvanced universal sybr green supermix, by Bio-Rad (2 mentions) Evos fl auto 2, by Thermo Fisher Scientific (2 mentions) Echo 550, by Beckman Coulter (2 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Cfx384 real time pcr instrument, by Bio-Rad (2 mentions) Nestin, by R&D Systems (2 mentions) Neurotrace 530 615, by Thermo Fisher Scientific (2 mentions) Immpress polymer based immunohistochemistry reagents, by Vector Laboratories (2 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (2 mentions) Vimentin, by Abcam (2 mentions)

Close spelling variants of this product

Confocal microscope (860 citations) 510 Meta confocal microscope (100 citations) LSM 510 laser confocal microscope (39 citations) 510 confocal laser scanning microscope (27 citations) 510 inverted confocal microscope (12 citations) LSM 510 inverted laser-scanning confocal microscope (7 citations) LCM 510 confocal microscope (7 citations) LSM 510 DUO confocal microscope (6 citations) Zeiss 510 NLO confocal microscope (4 citations) LSM 510 Carl Zeiss confocal microscope (3 citations)

104 protocols using 510 confocal microscope

1

Confocal Microscopy Immunofluorescence Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence was carried out as previously described. Cells were viewed on a Zeiss 510 confocal microscope under an oil immersion 63× objective lens. Images were analyzed using the LSM imaging software and are presented as z-stacked images (58 (link)).
Knight L.M., Stakaityte G., Wood J.J., Abdul-Sada H., Griffiths D.A., Howell G.J., Wheat R., Blair G.E., Steven N.M., Macdonald A., Blackbourn D.J, & Whitehouse A. (2014). Merkel Cell Polyomavirus Small T Antigen Mediates Microtubule Destabilization To Promote Cell Motility and Migration. Journal of Virology, 89(1), 35-47.
+ Open protocol
+ Expand
2

Quantifying Pancreatic Acinar Cell Vacuoles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freshly isolated pancreatic acinar cells were plated on to poly-L-lysine-coated glass-bottomed Petri dishes from MatTek. Extracellular solution was removed and quickly replaced with the incubation solution containing membrane-impermeable fluorescent probe Lucifer Yellow (2 mM), plus test compound(s) (e.g. CCK or TLC-S with or without a SOCE inhibitor) as specified in the Figures. Petri dishes were incubated for 1 h at 35°C. Cells were imaged on a Zeiss 510 confocal microscope with water-immersion objective ×63/numerical aperture 1.2; axial resolution was <1 μm. Fluorescence was excited using a 458 nm laser line; the emission was recorded at wavelengths longer than 505 nm. Z-stacks of confocal images (approximately 20 stacks/cell) were taken and the number of vacuoles counted. The numbers of individual experiments (at least three for each condition) are indicated in the Results and Discussion sections or in Figure legends. For these experiments, we set aside Petri dishes with attached acinar cells and separately counted vacuoles in unstimulated cells (control) and in cells stimulated by supramaximal concentrations of CCK or TLC-S. The effects of putative inhibitors or inducers of vacuole formation were normalized to the corresponding response induced by supramaximal CCK or TLC-S.
Voronina S., Collier D., Chvanov M., Middlehurst B., Beckett A.J., Prior I.A., Criddle D.N., Begg M., Mikoshiba K., Sutton R, & Tepikin A.V. (2015). The role of Ca2+ influx in endocytic vacuole formation in pancreatic acinar cells. Biochemical Journal, 465(Pt 3), 405-412.
+ Open protocol
+ Expand
3

Visualizing Carbon Nanoparticle Structures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Microscopy techniques were performed to visualize the structure of the carbon nanoparticles and the GOx-nanocomposites. A Zeiss 510 Confocal Microscope was used to conduct confocal analysis. In addition to confocal analysis, transmission electron microscopy (TEM) was carried out to visualize the distribution of the enzymes within the carbon nanoparticle network at a smaller scale. The TEM tests were conducted using a FEI TEM T20 microscopy at least in 5 different spots. The preparation of the samples was conducted using a similar step reported in a previous work (Garcia-Perez et al., 2016 (link)).
Garcia-Perez T., Hu S., Wee Y., Scudiero L., Hoffstater C., Kim J, & Ha S. (2019). Effect of Surface and Bulk Properties of Mesoporous Carbons on the Electrochemical Behavior of GOx-Nanocomposites. Frontiers in Chemistry, 7, 84.
+ Open protocol
+ Expand
4

Immunofluorescence Staining of TC32 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
TC32 cells were incubated with DMSO or trabectedin in a Nunc Lab-Tek II Chamber Slide (Thermo Scientific), fixed in 4% paraformaldehyde in PBS, washed, lysed in 1% Triton-X100, and blocked in 5% goat serum. Cells were incubated with primary antibody (18 h), secondary antibody (1 h) and DAPI (10 minutes), mounted in Vecta Shield mounting media (Vecta Laboratories);(Primary antibodies: nucleolin, Abcam – 1:1000; HA-tag, Abcam – 1:500; FLI1, Abcam – 1:100; N-terminal EWSR1, Cell Signaling – 1:1000) (Secondary antibodies: Cy5-conjugated anti-mouse IgG: Vector – 1:400, FITC-conjugated anti-Rabbit IgG: Millipore – 1:200) (DAPI Sigma Aldrich – 1:10,000). All images were obtained with standardized settings on a Zeiss 510 confocal microscope.
Harlow M.L., Chasse M.H., Boguslawski E.A., Sorensen K.M., Gedminas J.M., Kitchen-Goosen S.M., Rothbart S.B., Taslim C., Lessnick S.L., Peck A.S., Madaj Z.B., Bowman M.J, & Grohar P.J. (2019). Trabectedin Inhibits EWS-FLI1 and Evicts SWI/SNF from Chromatin in a Schedule Dependent Manner. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(11), 3417-3429.
+ Open protocol
+ Expand
5

Mitochondrial Superoxide Imaging in Hepatocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary hepatocytes isolated from MCUfl/fl and
MCUΔhep mice were plated on Poly-L-lysine coated glass
coverslips. Next day, cells were loaded with the mitochondrial superoxide
sensitive fluorophore MitoSOX Red (Life Technologies; 2 μM) and
Dihydrorhodamine 123 (Rhod123; 2.5 μg/ml) in William’s E
medium (without serum) at 37 C for 30 min. Cells were then washed and imaged
using a Carl Zeiss 510 confocal microscope with a 40 3 oil immersion
objective at 561 nm as described earlier (Mukhopadhyay et al., 2007 (link)). MitoSOX fluorescence was quantified
using ImageJ software and plotted as arbitrary units in Graphpad Prism 6
software.
Tomar D., Jaña F., Dong Z., Quinn WJ I.I.I., Jadiya P., Breves S.L., Daw C.C., Srikantan S., Shanmughapriya S., Nemani N., Carvalho E., Tripathi A., Worth A.M., Zhang X., Razmpour R., Seelam A., Rhode S., Mehta A.V., Murray M., Slade D., Ramirez S.H., Mishra P., Gerhard G.S., Caplan J., Norton L., Sharma K., Rajan S., Balciunas D., Wijesinghe D.S., Ahima R.S., Baur J.A, & Madesh M. (2019). Blockade of MCU-Mediated Ca2+ Uptake Perturbs Lipid Metabolism via PP4-Dependent AMPK Dephosphorylation. Cell reports, 26(13), 3709-3725.e7.
+ Open protocol
+ Expand
6

Confocal Imaging of Larval NMJs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Larval NMJs were imaged on a Zeiss 510 confocal microscope at 63× and 100×. Brightness and contrast were adjusted in Adobe Photoshop CS5. For all experiments comparing levels of fluorescence, larval body walls were stained in the same tube and brightness/contrast were not altered. To normalize Gbb-HA or Cmpy-Venus protein levels, mean fluorescence levels after subtraction of average background were divided by mean HRP fluorescence. For data analysis, groups of means were compared by one-way ANOVA, and pairs of means by the unpaired Student's t-test.
James R.E., Hoover K.M., Bulgari D., McLaughlin C.N., Wilson C.G., Wharton K.A., Levitan E.S, & Broihier H.T. (2014). Crimpy enables discrimination of pre and postsynaptic pools of a BMP at the Drosophila NMJ. Developmental cell, 31(5), 586-598.
+ Open protocol
+ Expand
7

Measuring Intracellular ROS Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
ROS levels were measured using CellRox Deep Red Reagent (Molecular Probes) as an indicator of intracellular ROS. For microscopic analysis, GFP labeled SKOV3ip1 cells were cultured with CAFs or alone on poly-D-lysine coated cover glass for 4 hr. Cells were then loaded with 5 μM CellRox Deep Red for 30 min, washed with PBS and imaged live using a Zeiss 510 confocal microscope. Quantification from 3 independent experiments is shown. Representative images are shown.
Curtis M., Kenny H.A., Ashcroft B., Mukherjee A., Johnson A., Zhang Y., Helou Y., Batlle R., Liu X., Gutierrez N., Gao X., Yamada S.D., Lastra R., Montag A., Ahsan N., Locasale J.W., Salomon A.R., Nebreda A.R, & Lengyel E. (2018). Fibroblasts mobilize tumor cell glycogen to promote proliferation and metastasis. Cell metabolism, 29(1), 141-155.e9.
+ Open protocol
+ Expand
8

Mitochondrial Membrane Potential Imaging

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were plated in six-well plates containing 0.2% gelatin–coated glass coverslips (Thapa et al., 2011 (link)), and 100 nM TMRE perchlorate was added to cells and incubated for 30 min at 37°C. Nuclear DNA stain, 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole (Hoescht 33342), was added for 5 min. Image acquisition was performed using a Carl Zeiss 510 confocal microscope using a 63× oil objective with excitation at 561 and 405 nm, respectively. Images were quantified using ImageJ (Madesh et al., 2005 (link)).
Hoffman N.E., Chandramoorthy H.C., Shanmughapriya S., Zhang X.Q., Vallem S., Doonan P.J., Malliankaraman K., Guo S., Rajan S., Elrod J.W., Koch W.J., Cheung J.Y, & Madesh M. (2014). SLC25A23 augments mitochondrial Ca2+ uptake, interacts with MCU, and induces oxidative stress–mediated cell death. Molecular Biology of the Cell, 25(6), 936-947.
+ Open protocol
+ Expand
9

Glycogen Visualization in Cancer-CAF Interaction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cancer cells were seeded in 24-well plates and primary CAFs were seeded on poly-D-lysine coated cover glass. The next day media was removed from cancer cells and replaced with glucose-free DMEM (Life Technologies). Glycogen was visualized using a previously described method with modifications (Louzao et al., 2008 (link)). After 30 min 2-NBDG (Caymen Chemical) was added at 500 μM. Cells were incubated for 2 hr with 2-NBDG, then all media was removed and the cells were collected via trypsinization. CAFs were washed one time with serum-free DMEM and then the 2-NBDG labeled cancer cells were plated with CAFs or alone on poly-D-lysine coated coverslips. After 4 hours the live cells were imaged using a Zeiss 510 confocal microscope. Images are representative of 2 technical replicates from 3 independent experiments.
Curtis M., Kenny H.A., Ashcroft B., Mukherjee A., Johnson A., Zhang Y., Helou Y., Batlle R., Liu X., Gutierrez N., Gao X., Yamada S.D., Lastra R., Montag A., Ahsan N., Locasale J.W., Salomon A.R., Nebreda A.R, & Lengyel E. (2018). Fibroblasts mobilize tumor cell glycogen to promote proliferation and metastasis. Cell metabolism, 29(1), 141-155.e9.
+ Open protocol
+ Expand
10

Immunohistochemical Analysis of Epigenetic Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in ice cold methanol/acetic acid (3:1). The fixed cells were dropped on microscope slides. Antigen retrieval was performed followed by blocking for 10 min at room temperature. Primary antibody was incubated overnight at 4 °C for the following antigens: ZSCAN4 (Origene, 1:1000), RNF20 (Cell Signaling, 1:2000), and Ubiquitin Lysine 48 (Millipore 1: 2000). The slides were washed and incubated with secondary antibodies conjugated with Alexa-488 or 568 at room temperature for 1 h and then treated with DAPI and To-Pro-3 to stain the nuclei. Slides were mounted and visualized by a Zeiss 510-confocal microscope. Co-localization analyses were performed by ImageJ software [16 (link)].
Portney B.A., Khatri R., Meltzer W.A., Mariano J.M, & Zalzman M. (2018). ZSCAN4 is negatively regulated by the ubiquitin-proteasome system and the E3 ubiquitin ligase RNF20. Biochemical and biophysical research communications, 498(1), 72-78.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!