Prism 6.0f

Macrophage infectivity and ectocervical explant infectivity were evaluated in an unmodified format (FFU/mL) and a normalized format (% of TZM-bl). In both cases the data were summarized as mean and standard error of the mean for each pseudovirus. Comparisons were also made among three groups of pseudoviruses, late-disease macrophage-tropic, late-disease non-macrophage-tropic, and transmitted/founder/acute. To achieve normality within groups the data were log transformed. Then groups were compared by unpaired two-tailed t test in Prism 6.0f. Groups are presented with their geometric mean and 95% confidence intervals (Figures 1, 3).
Data for ectocervical explant stimulation methods and maraviroc and nevirapine treatment are mean and standard error (Figure 2). Data for ectocervical cell selections are presented with mean and 95% confidence intervals with group comparisons tested by the Wilcoxon matched-pairs signed rank test in Prism 6.0f (Figure 4).

This was a pilot study to assess the impact of ASA on T cell immune activation. An intention to treat analysis was performed. Chi Squared (X²) was used to assess the significance of the associations between categorical variable using Prism 6.0f (GraphPad Software, La Jolla, CA, USA) and Gaussian distribution was tested by Shapiro-Wilk normality test and normality plot using SPSS (NY, USA). To compare baseline to visit 3 two-tailed paired T test (for normally distributed data) or Wilcoxon matched-pair signed-rank test (for data not following normality distribution) were performed using Prism 6.0f (GraphPad Software, La Jolla, CA, USA) or STATA v15.0 (StataCorp LLC, USA). Using SSPS, multivariate regressions were performed to assess the impact of DMPA usage and age on the change score (Visit 3-baseline). To understand the dynamics of the immune system between systemic and musical compartments we performed correlations between plasma and CVL ASA levels were run against data from both the PBMCs and CMCs. Pearson correlation or Spearman’s rank test were used for correlations between continuous variables (GraphPad Software, La Jolla, CA, USA). A p-value of <0.05 was considered as significant correction for multiple comparisons was not performed. Participants who were discontinued from the study were excluded from the analysis.

Statistical significance for the morphological, gene expression and protein level studies was evaluated by the use of graphpad prism 6.0f (Graphpad Software, La Jolla, CA, USA), with paired or unpaired two‐tailed Student's t‐tests; for the proliferation studies, a two‐way anova followed by Sidak's multiple comparison test was performed. Levels of significance were expressed as P‐values.