VWF:Ag levels were measured using immunoturbidometric assay (HemosIL von Willebrand Factor Antigen, Instrumentation Laboratory, Bedford, MA). Platelet-dependent VWF activity was measured using VWF:GPIbR (HemosIL von Willebrand Factor Ristocetin Cofactor Activity, Instrumentation Laboratory, Bedford, MA). FVIII clotting activity (FVIII:C) was assessed using a 1-stage assay and VWF collagen binding (VWF:CB) with collagen type I, III, or a combination of thereof using an enzyme-linked immunosorbent assay method.12 (link) VWF multimer analysis was performed using a semiautomatic Hydrasys 2 scan (Sebia, Lisses, France), and we used the HYDRAGEL 5 von WILLEBRAND MULTIMERS kit.13 (link) A commercially available enzyme-linked immunosorbent assay kit (Sanquin, Amsterdam, The Netherlands) was used to measure the VWF propeptide (VWFpp) antigen.14 (link) To evaluate the synthesis and secretion of VWF we used the FVIII:C/VWF:Ag ratio and VWFpp.14 (link) VWF clearance was assessed using the VWFpp/VWF:Ag ratio with a ratio exceeding 1.6 considered an indicator of accelerated clearance.15 (link)
Hemosil von willebrand factor antigen
Manufactured by Werfen
Sourced in United States
The HemosIL Von Willebrand Factor Antigen is a lab equipment product used for the quantitative determination of von Willebrand factor antigen in human plasma. It is intended for in vitro diagnostic use.
Automatically generated - may contain errors
Lab products found in correlation
Hydrasys 2 scan, by Sebia (1 mentions)
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Zymutest pai 1 antigen, by Hyphen Biomed (1 mentions)
Zymutest t pa antigen, by Hyphen Biomed (1 mentions)
Human se selectin cd62e quantikine elisa kit, by R&D Systems (1 mentions)
Human thrombomodulin bdca 3 quantikine elisa kit, by R&D Systems (1 mentions)
3 protocols using hemosil von willebrand factor antigen
1
Comprehensive VWF Functional Assays
2
Complement, Coagulation, and Endothelial Activation Markers
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Plasma levels of soluble C5b-9 (SC5b-9) were measured as a marker of complement activation using a solid-phase assay (MicroVue Complement SC5b-9 Plus EIA kit, Quidel Corporation, San Diego, CA, USA) whose intra- and inter-assay coefficients of variation (CVs) were, respectively, 6.8% and 13.1%; the lower detection limit was 3.7 ng/mL.
D-dimer levels were measured as markers of coagulation activation via automated latex agglutination tests using anti-D-dimer monoclonal antibodies according to Kumano et al. [22 (link)]. The intra- and inter-assay coefficients of variation were lower than 3.8%.
Plasma levels of von Willebrand factor (vWF) antigen were measured as markers of endothelial activation using a commercial method (HemosIL Von Willebrand Factor Antigen, Instrumentation Laboratory, Bedford, MA, USA) with intra- and inter-assay CVs of <2.3%; the lower detection limit was 2.2%.
D-dimer levels were measured as markers of coagulation activation via automated latex agglutination tests using anti-D-dimer monoclonal antibodies according to Kumano et al. [22 (link)]. The intra- and inter-assay coefficients of variation were lower than 3.8%.
Plasma levels of von Willebrand factor (vWF) antigen were measured as markers of endothelial activation using a commercial method (HemosIL Von Willebrand Factor Antigen, Instrumentation Laboratory, Bedford, MA, USA) with intra- and inter-assay CVs of <2.3%; the lower detection limit was 2.2%.
3
Plasma Biomarkers of Endothelial Function
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Plasma levels of von Willebrand factor (vWF) antigen were measured using a commercial method (HemosIL Von Willebrand Factor Antigen, Instrumentation Laboratory, Bedford, MA, USA) with intra- and inter-assay CVs of <2.3%; the lower detection limit was 2.2%.
Tissue-type plasminogen activator (t-PA) antigen was measured in plasma using a commercially available ELISA (Zymutest t-PA antigen, Hyphen BioMed, Neuville sur Oise, France) in accordance with the manufacturer's instructions. The intra- and inter-assay CVs were <10%, and the lower detection limit was 0.5 ng/mL.
Plasminogen activator inhibitor-1 (PAI-1) antigen was measured in plasma using a commercially available ELISA (Zymutest PAI-1 antigen, Hyphen BioMed) whose intra- and inter-assay CVs were 8% and 13%, respectively; the lower detection limit was 0.5 ng/mL.
Soluble plasma thrombomodulin (sTM) plasma levels were measured using a commercial sandwich ELISA (Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit, R&D Systems, Minneapolis, MN, USA) whose intra- and inter-assay CVs were both <10%; the lower detection limit was 7.82 pg/mL.
Soluble endothelial selectin (sE-selectin) was measured in plasma using a sandwich ELISA (Human sE-Selectin/CD62E Quantikine ELISA Kit, R&D Systems Inc.) whose intra- and inter-assay CVs were respectively 5% and 8.8%; the lower detection limit was 0.009 ng/mL.
Tissue-type plasminogen activator (t-PA) antigen was measured in plasma using a commercially available ELISA (Zymutest t-PA antigen, Hyphen BioMed, Neuville sur Oise, France) in accordance with the manufacturer's instructions. The intra- and inter-assay CVs were <10%, and the lower detection limit was 0.5 ng/mL.
Plasminogen activator inhibitor-1 (PAI-1) antigen was measured in plasma using a commercially available ELISA (Zymutest PAI-1 antigen, Hyphen BioMed) whose intra- and inter-assay CVs were 8% and 13%, respectively; the lower detection limit was 0.5 ng/mL.
Soluble plasma thrombomodulin (sTM) plasma levels were measured using a commercial sandwich ELISA (Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit, R&D Systems, Minneapolis, MN, USA) whose intra- and inter-assay CVs were both <10%; the lower detection limit was 7.82 pg/mL.
Soluble endothelial selectin (sE-selectin) was measured in plasma using a sandwich ELISA (Human sE-Selectin/CD62E Quantikine ELISA Kit, R&D Systems Inc.) whose intra- and inter-assay CVs were respectively 5% and 8.8%; the lower detection limit was 0.009 ng/mL.
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