Sleep quality was objectively assessed by Actigraph accelerometer worn on the right ASIS by the participants for the initial 3 days of the 1st week and last 3 days of the 3rd and 7th weeks. The parameters assessed were—sleep efficiency (SE), total sleep time (TST), and sleep onset latency (SOL). TST is the total duration of sleep during the 24 h, measured in minutes. SE is the percentage of total sleep time to the total time spent in bed. SOL is the time taken to fall asleep once in bed and is measured in minutes. Along with the objective values, subjective bedtime and wake up time were obtained from the participants on the days of accelerometer analysis.
Circadian rhythm was assessed by melatonin levels in the first morning void urine samples collected in the 1st, 3rd, and 7th week of chemoradiotherapy. The samples were stored at a temperature of −20°C and the analysis was performed using Elabscience ELISA kit.