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Alexa fluor 647 anti mouse b220 antibody clone ra3 6b2

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The Alexa Fluor 647 anti-mouse B220 antibody (clone RA3–6B2) is a fluorescently labeled monoclonal antibody that recognizes the B220 cell surface antigen expressed on mouse B cells. This antibody can be used for the identification and enumeration of B cell populations in flow cytometry applications.

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Lab products found in correlation

Tcs sp5 microscope, by Leica (2 mentions) Imaris software 9, by Oxford Instruments (2 mentions)

Close spelling variants of this product

B220-Alexa Fluor 647 (RA3-6B2 Anti-mouse B220 (clone RA3-6B2, Anti-B220 (clone RA3-6B2, B220 (clone RA3-6B2, Anti-B220 (RA3-6B2, B220 (RA3-6B2, B220–Alexa Fluor 647 Clone RA3-6B2 RA3-6B2 Alexa Fluor 647

2 protocols using alexa fluor 647 anti mouse b220 antibody clone ra3 6b2

1

Visualization of IL-21 and IL-2 Expression

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Tissues were harvested after perfusing Il21-mCherry/Il2-emGFP TG mice with 4% paraformaldehyde (PFA, pH 7.4 at 4 °C), tissues were further fixed in 4% PFA at 4°C for 2 hours, placed in 30% sucrose for 24 hours, and embedded in OCT compound (Tissue-Tek) for freezing. 10-µm thick frozen tissue sections were rehydrated, blocked with 10% rat serum in 0.1% Tween20/PBS (PBST) for 1 hour at room temperature, and stained with the Alexa Fluor 647 anti-mouse B220 antibody (clone RA3–6B2, Biolegend) in 1% rat serum/ 0.1% PBST overnight at 4 °C. Nuclei were stained with 4’,6-diamidin-2-fenilindolo (DAPI). Images were acquired using a Leica TCS SP5 microscope (Leica Microsystems, Mannheim, Germany) using a 20x objective NA .7, zoom 1X and processed with Imaris software 9.0.0 (Bitplane AG, Zurich Switzerland).
Cho H., Jaime H., Oliveira R.P., Kang B., Spolski R., Vaziri T., Myers T.G., Thovarai V., Shen Z., Fox J.G., Leonard W.J, & Kelsall B.L. (2018). Defective IgA response to atypical intestinal commensals in IL-21 receptor deficiency reshapes immune cell homeostasis and mucosal immunity. Mucosal immunology, 12(1), 85-96.
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2

Visualization of IL-21 and IL-2 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were harvested after perfusing Il21-mCherry/Il2-emGFP TG mice with 4% paraformaldehyde (PFA, pH 7.4 at 4 °C), tissues were further fixed in 4% PFA at 4°C for 2 hours, placed in 30% sucrose for 24 hours, and embedded in OCT compound (Tissue-Tek) for freezing. 10-µm thick frozen tissue sections were rehydrated, blocked with 10% rat serum in 0.1% Tween20/PBS (PBST) for 1 hour at room temperature, and stained with the Alexa Fluor 647 anti-mouse B220 antibody (clone RA3–6B2, Biolegend) in 1% rat serum/ 0.1% PBST overnight at 4 °C. Nuclei were stained with 4’,6-diamidin-2-fenilindolo (DAPI). Images were acquired using a Leica TCS SP5 microscope (Leica Microsystems, Mannheim, Germany) using a 20x objective NA .7, zoom 1X and processed with Imaris software 9.0.0 (Bitplane AG, Zurich Switzerland).
Cho H., Jaime H., Oliveira R.P., Kang B., Spolski R., Vaziri T., Myers T.G., Thovarai V., Shen Z., Fox J.G., Leonard W.J, & Kelsall B.L. (2018). Defective IgA response to atypical intestinal commensals in IL-21 receptor deficiency reshapes immune cell homeostasis and mucosal immunity. Mucosal immunology, 12(1), 85-96.
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