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The Hi GC is a gas chromatography (GC) system designed for reliable and efficient sample analysis. It features advanced technology for precise separation, detection, and quantification of complex chemical mixtures. The core function of the Hi GC is to provide accurate and reproducible results for a wide range of applications.

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Lab products found in correlation

Neon transfection system, by Thermo Fisher Scientific (2 mentions) Stealth rnai negative universal control lo gc, by Thermo Fisher Scientific (2 mentions) Med gc, by Thermo Fisher Scientific (2 mentions) Mouse macrophage nucleofector kit, by Lonza (1 mentions) Spi1 hss186060, by Thermo Fisher Scientific (1 mentions) Nucleofector 2, by Lonza (1 mentions)

2 protocols using hi gc

1

Transfection of siRNAs for PU.1 and IRF Transcription Factors

Cited 2 times
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Small interfering RNAs for mouse PU.1 (Spi1-MSS247676), human PU.1 (Spi1-HSS186060), mouse IRF4 (MSS205501), and mouse IRF8 (MSS236848) and control siRNA (Stealth RNAi Negative Universal Control Lo GC, Med GC, and Hi GC (#12935–200, 300, and 400)) were purchased from Invitrogen (Carlsbad, CA). BMMCs of 2 × 106 (or 2 × 105) were transfected with 10 (or 1) μl of 20 μmol/L siRNA with a Neon 100 μl kit (or a Neon 10 μl kit) using a Neon transfection system (Invitrogen) set at Program #5. Introduction of siRNA into LAD2 cells and BMDCs was performed as previously described15 (link),24 (link). Briefly, a Neon transfection system was used for LAD2 cells15 (link), and a Mouse Macrophage Nucleofector kit and Nucleofector II (Lonza, Basel, Switzerland) were used for BMDCs25 (link),26 (link).
Oda Y., Kasakura K., Fujigaki I., Kageyama A., Okumura K., Ogawa H., Yashiro T, & Nishiyama C. (2018). The effect of PU.1 knockdown on gene expression and function of mast cells. Scientific Reports, 8, 2005.
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2

siRNA Transfection of BMMCs

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The following small interfering RNAs (siRNAs) were purchased from Invitrogen (Carlsbad, CA); mouse Gata1 (MSS236579), Gata2 (MSS204585), Smad2 (MSS206405), Smad3 (MSS206420), and Smad4 (MSS206437) and control siRNAs (Stealth RNAi Negative Universal Control Lo GC, Med GC, and Hi GC [no. 12935–200, −300, and −400]). BMMCs (2 × 106 or 2 × 105) were transfected with 10 or 1 μl of 20 μmol/L siRNA with a Neon 100 μl kit or a Neon 10 μl kit using a Neon transfection system (Invitrogen) set at Program no. 5.
Kasakura K., Nagata K., Miura R., Iida M., Nakaya H., Okada H., Arai T., Arai T., Kawakami Y., Kawakami T., Yashiro T, & Nishiyama C. (2020). Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors. Journal of immunology (Baltimore, Md. : 1950), 204(6), 1641-1649.
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