Brain slides were blocked with 1% BSA/PBS for 1 h at RT and incubated with anti-MBP (1:500, Abcam, Burlingame, CA, USA), anti-Iba-1 (1:300, BD Bioscience, USA), anti-NF-κB/p65 (1:200, Cell Signaling Technology, Danvers, MA, USA), anti-TLR4 (1:200, Bioworld Tech. Inc., St. Louis Park, MN, USA), anti-iNOS (1:300, Abcam, Burlingame, CA, USA), anti-Arg-1 (1:300, Gene Tex, Irvine, CA, USA), anti-GFAP (1:250, Thermo Fisher, USA), anti-BDNF (1:200, Novusbio, Centennial, CO, USA), anti-GDNF (1:500, Abcam, Burlingame, CA, USA), anti-NG2 (1:300, Millipore, Germany) and anti-Ki67 (1:200, BD Pharmingen, USA) at 4°C for overnight, followed by corresponding secondary antibodies at RT for 1 h. The results were repeated three times with consecutive brain slices from each group, and the slides were observed under fluorescence microscopy in a blinded fashion. Analysis and quantification were done in three sections/per mouse by Image-Pro Plus 6.0 software.
Anti bdnf
Manufactured by Novus Biologicals
Sourced in United States
Anti-BDNF is a laboratory reagent used in research applications. It functions as an antibody that specifically binds to brain-derived neurotrophic factor (BDNF), a protein involved in neuronal growth and development. This product can be used in various experimental techniques to study the role and interactions of BDNF.
Automatically generated - may contain errors
Lab products found in correlation
Anti iba 1, by BD (2 mentions)
Anti gfap, by Thermo Fisher Scientific (2 mentions)
Anti gdnf, by Abcam (2 mentions)
Anti arg 1, by GeneTex (2 mentions)
Anti nf κb p65, by Cell Signaling Technology (2 mentions)
Anti inos, by Abcam (2 mentions)
Anti ki67, by BD (2 mentions)
Anti ng2, by Merck Group (1 mentions)
Anti mbp, by Abcam (1 mentions)
Anti pdgfrα, by Merck Group (1 mentions)
Abi 7500, by Thermo Fisher Scientific (1 mentions)
Trizol kit, by Thermo Fisher Scientific (1 mentions)
Anti trkb, by Abcam (1 mentions)
M mlv reverse transcription kit, by Promega (1 mentions)
Semi dry transfer instrument, by Bio-Rad (1 mentions)
Anti p75 ngf receptor, by Abcam (1 mentions)
Anti gapdh, by Abcam (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Pdvf membrane, by Merck Group (1 mentions)
Rabbit anti rat β actin, by Abbkine (1 mentions)
4 protocols using anti bdnf
1
Immunohistochemical Analysis of Neuroinflammation
2
Immunofluorescence Analysis of Neural Markers
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Cells were blocked with 1% BSA/PBS for 1 h at RT and incubated with anti-Iba-1 (1:300, BD Bioscience, San Jose, CA, USA), anti-NF-κB/p65 (1:200, Cell Signaling Technology, USA), anti-iNOS (1:300, Abcam, Burlingame, CA, USA), anti-Arg-1 (1:300, Gene Tex, Irvine, CA, USA), anti-GFAP (1:250, Thermo Fisher, USA), anti-BDNF (1:200, Novusbio, Centennial, CO, USA), anti-GDNF (1:500, Abcam, USA), anti-PDGFRα (1:300, Millipore, Germany) and anti-Ki67 (1:200, BD Pharmingen, USA) at 4°C for overnight, followed by corresponding secondary antibodies at RT for 1 h. The results were repeated three times with similar results. Analysis and quantification were done by Image-Pro Plus 6.0 software.
3
Molecular Mechanisms of Antidepressant Effects
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Anti-tPA (Novus Biologicals, Centennial, CO, USA), anti-BDNF (Novus Biologicals), anti-TrkB (Abcam, Cambridge, MA, USA), anti-ProBDNF (Novus Biologicals), anti-p75NGF receptor (Abcam), anti-GAPDH (Abcam), fluoxetine hydrochloride capsule (French Patheon, Bourgoin, France), TRIzol kit (Invitrogen, Carlsbad, CA, USA), polymerase chain reaction (PCR) primer (Bio-Bioengineering Shanghai Co. Ltd., Shanghai Shi, China), M-MLV reverse transcription kit (Promega, Madison, WI, USA), real-time PCR amplification kit (Beijing Zhongyuan Leading Technology Co. Ltd., Beijing, China), stabilized flow electrophoresis system (Bio-Rad Laboratories, Hercules, CA, USA), semi-dry transfer instrument (Bio-Rad Laboratories), real-Time PCR instrument (ABI 7500; Thermo Fisher Scientific, Waltham, MA, USA), electronic balance (BS224S; Satorius, Göttingen, Germany), nucleic acid UV spectrometry photometer (Eppendorf, Hamburg, Germany), and an open field test box (self-made; 60 cm × 60 cm × 40 cm).
4
Hippocampal Protein Extraction and Western Blot Analysis
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The total protein was extracted from hippocampus tissue using a radio-immunoprecipitation assay (RIPA) buffer containing protease inhibitors (Abmole biotechnology, Shanghai, China). The 20 μg extracted proteins were separated by 10% SDS-PAGE (Bio-Rad) and transferred onto polyvinylidene fluoride (PDVF) membrane (Millipore, Billerica, MA, USA). The membrane was blocked with 5% nonfat dry milk for 2 h and incubated overnight at 4°C with the relevant primary antibodies. The primary antibodies were anti-BDNF(1 : 1000, novus biologicals, Beijing, China), anti-caspase-3 (1 : 1000, Affinity, Liyang, China), anti-Bax (1 : 1000, Abcam, Cambridge, UK), anti-Bcl-2 (1 : 1000, Abcam, Cambridge, UK), anti-p-CREB (1 : 1000, Cell Signaling Technology, MA, USA), anti-TrkB(1 : 1000, Cell Signaling Technology, MA, USA), and anti-p-Akt (1 : 2000, Cell Signaling Technology, MA, USA). The membrane was incubated with the horseradish peroxidase (HRP)-conjugated goat anti-rabbit immunoglobulin G (IgG) (1 : 20,000, Abbkine, Wuhan, China). The membrane was visualized by the proteins by ECL reagent and analyzed by ImageJ software. Rabbit anti-rat β-actin (1 : 2000, Abbkine, Wuhan, China) was used as an internal control.
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