Isradipine

Manufactured by Alomone

Sourced in Israel

Isradipine is a synthetic organic compound that functions as a calcium channel blocker. It is used in various laboratory applications, such as research on cardiovascular and neurological processes.

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Lab products found in correlation

6 protocols using isradipine

Cocaine-Induced Conditioned Place Preference

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For cocaine conditioned place preference (CPP) experiments, cocaine hydrochloride (Sigma-Aldrich) was dissolved in 0.9% saline at a concentration of 1.0 mg/ml to be injected intraperitoneally (i.p.) at 0.01 ml/g body weight for a final dosage of 10 mg/kg. Isradipine (Alomone Labs) was dissolved in saline (16% ethanol) at a concentration of 0.12 mg/ml to be injected i.p. at 0.01 ml/g body weight for a final dosage of 1.2 mg/kg. Isradipine or vehicle (16% ethanol in saline) was injected 25 minutes prior to behavioral testing. For chemogenetic experiments, clozapine-N-oxide (CNO, Enzo Life Sciences) was dissolved in saline at a concentration of 0.3 mg/ml to be injected I.P. at 0.01 ml/g body weight for a final dosage of 3.0 mg/kg. CNO or saline was injected 45 minutes prior to behavioral testing.

Bavley C.C., Fetcho R.N., Burgdorf C.E., Walsh A., Fischer D.K., Hall B.S., Sayles N.M., Contoreggi N.H., Hackett J., Antigua S., Babij R., Kash T.L., Milner T.A., Liston C, & Rajadhyaksha A. (2019). Cocaine- and stress-primed reinstatement of drug-associated memories elicit differential behavioral and frontostriatal circuit activity patterns via recruitment of L-type Ca2+ channels. Molecular psychiatry, 25(10), 2373-2391.

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Evaluation of Cellular Metabolism Modulators

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All reagents were purchased from ThermoFisher (Waltham, MA, USA) unless otherwise stated. TOFA (5-(tetradecyloxy)-2-furoic acid^{13 (link)}; ab141578) and Tolbutamide were purchased from Abcam; Noradrenaline from Tocris Bioscience (Bristol, UK); 2-Bromopalmitate from Sigma-Aldrich; Tetrodotoxin, ω-agatoxin and isradipine from Alomone Labs (Jerusalem, Israel).

Veprik A., Denwood G., Liu D., Bany Bakar R., Morfin V., McHugh K., Tebeka N.N., Vetterli L., Yonova-Doing E., Gribble F., Reimann F., Hoehn K.L., Hemsley P.A., Ahnfelt-Rønne J., Rorsman P., Zhang Q., de Wet H, & Cantley J. (2022). Acetyl-CoA-carboxylase 1 (ACC1) plays a critical role in glucagon secretion. Communications Biology, 5, 238.

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Electrophysiological Experiments Protocols

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All electrophysiological experiments were performed as described previously^{23 (link)}. Agatoxin, isradipine, SNX 482, stromatoxin and iberiotoxin were purchased from Alomone (Jerusalem, Israel) and tetrodotoxin (TTX) from Sigma (Gillingham, UK).

Hastoy B., Godazgar M., Clark A., Nylander V., Spiliotis I., van de Bunt M., Chibalina M.V., Barrett A., Burrows C., Tarasov A.I., Scharfmann R., Gloyn A.L, & Rorsman P. (2018). Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells. Scientific Reports, 8, 16994.

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Intracellular Calcium Regulation Assay

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All chemicals were obtained from Sigma except for cyclopiazonic acid (CPA (Alomone), thapsigargin and 1,4-dihydroxy-2,5-di-tert-butylbenzene (BHQ, Tocris), Fluo-5F, AM (Invitrogen), ionomycin (Alomone) and VGCC blockers isradipine, ω-conotoxin-GVIA and ω-Agatoxin IVA (Alomone).

de Juan-Sanz J., Holt G.T., Schreiter E.R., de Juan F., Kim D.S, & Ryan T.A. (2017). Axonal endoplasmic reticulum Ca2+ content controls release probability in CNS nerve terminals. Neuron, 93(4), 867-881.e6.

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Investigating Glucagon-like Peptide Signaling

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GLP-1(7–36), GLP-1(9–36), exendin-4 and exendin(9–39) were from Bachem (Weil am Rhein, Germany), the glucagon receptor (GCGR) antagonist REMD2.59 was from REMD Bioptherapeutics (Camarillo, CA, USA) and pertussis toxin (PTX) was from Sigma (Gillingham, Dorset, UK). The Ca²⁺ channel blockers ω-agatoxin and isradipine, respectively blocking voltage-gated Ca²⁺ channels sensitive to ω-agatoxin (P/Q-type Ca²⁺ channels) and L-type Ca²⁺ channels, were from Alomone Labs (Jerusalem, Israel). The glucagon receptor antagonists (GRA) L-168049 and REMD2.59 were from Tocris Bioscience (Bristol, UK) and REMD Biotherapeutics Inc (Camarillo, CA, USA), respectively. Sitagliptin was obtained from Stratech Scientific (Ely, UK). The protein kinase A (PKA) inhibitor 8-Br-Rp-cAMPS was purchased from BioLog Life Science Institute (Bremen, Germany).

Gandasi N.R., Gao R., Kothegala L., Pearce A., Santos C., Acreman S., Basco D., Benrick A., Chibalina M.V., Clark A., Guida C., Harris M., Johnson P.R., Knudsen J.G., Ma J., Miranda C., Shigeto M., Tarasov A.I., Yeung H.Y., Thorens B., Asterholm I.W., Zhang Q., Ramracheya R., Ladds G, & Rorsman P. (2023). GLP-1 metabolite GLP-1(9–36) is a systemic inhibitor of mouse and human pancreatic islet glucagon secretion. Diabetologia, 67(3), 528-546.

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Modulation of Ca2+ Signaling Pathways

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The following substances were used (source given in parentheses): the L-type Ca²⁺ channel blocker isradipine and the P/Q Ca²⁺ channel blocker ω-agatoxin (Alomone Labs; Jerusalem, Israel); the α₁-antagonist prazosin (Abcam, Cambridge, UK); the membrane-permeable PKA inhibitor myr-PKI, the IP₃ receptor inhibitor Xestospongin C, the NAADP antagonist Ned-19, the V-ATPase inhibitor bafilomycin, the sER ATPase inhibitor thapsigargin and noradrenaline (Tocris Bioscience, Bristol, UK); the EPAC2 inhibitor ESI-05 (BioLog, Bremen, Germany); the insulin receptor antagonist (S961, Novo-Nordisk, Denmark). All other compounds were obtained from Sigma-Aldrich (Dorset, UK).

Hamilton A., Zhang Q., Salehi A., Willems M., Knudsen J.G., Stephen S., Ringgaard A.K., Chapman C.E., Gonzalez-Alvarez A., Surdo N.C., Zaccolo M., Basco D., Johnson P.R., Ramracheya R., Rutter G.A., Galione A., Rorsman P, & Tarasov A.I. (2018). Adrenaline stimulates glucagon secretion by Tpc2-dependent Ca2+ mobilization from acidic stores in pancreatic α-cells. Diabetes, 67(6), 1128-1139.

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