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Agn193109

Manufactured by Bio-Techne
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Sourced in United Kingdom, Germany

AGN193109 is a selective androgen receptor modulator (SARM) compound developed by Bio-Techne. It is designed to selectively bind and activate the androgen receptor, which plays a role in various physiological processes. The core function of AGN193109 is to serve as a tool compound for research and laboratory studies, without interpretation or extrapolation on its intended use.

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Lab products found in correlation

Retinoic acid, by Bio-Techne (1 mentions) Anti pd l1, by Cell Signaling Technology (1 mentions) Anti c myc, by Cell Signaling Technology (1 mentions) Anti sox2, by Cell Signaling Technology (1 mentions) Anti oct4a, by Cell Signaling Technology (1 mentions) Cellytic mt, by Merck Group (1 mentions) Uvi3003, by Bio-Techne (1 mentions) Anti nanog, by Cell Signaling Technology (1 mentions) Anti klf4, by Cell Signaling Technology (1 mentions) Anti mpges 1, by Cayman Chemical (1 mentions) Anti β actin, by Merck Group (1 mentions) Vorinostat, by Merck Group (1 mentions) Metformin hydrochloride, by Merck Group (1 mentions) Sml0061, by Merck Group (1 mentions) D8375, by Merck Group (1 mentions)

5 protocols using agn193109

1

Retinoic Acid Receptor Activation Assay

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Retinoic acid (Tocris, 695), Ch 55, Potent RAR agonist (Tocris, 2020), and AGN 193109, High affinity pan-RAR antagonist (Tocris, 5758) were dissolved in dimethyl sulfoxide and added to fresh culture media without rosiglitazone to feed every 3-4 days for a final concentration of 1 μM. Cultures were differentiated for 9-16 days prior to treatment. For dose-response studies, cultures were differentiated for 20 days and then treated every day for an additional 20 days with the indicated concentrations of Retinoic acid receptor antagonist in fresh culture media.
Pope B.D., Warren C.R., Dahl M., Pizza C.V., Henze D.E., Sinatra N.R., Gonzalez G.M., Chang H., Liu Q., Glieberman A.L., Ferrier JP J.r., Cowan C.A, & Parker K.K. (2020). Fattening Chips: Hypertrophy, Feeding, and Fasting of Human White Adipocytes In Vitro. Lab on a chip, 20(22), 4152-4165.
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2

Stemness and Immune Markers Assay

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Where indicated, cells were treated with RAR antagonist AGN193109 (5758, Tocris Bioscience, Bristol, UK) and RXR antagonist UVI3003 (3303, Tocris Bioscience) for 48 h (1 µM).
Total protein lysates were obtained using celLytic MT (C2978-50 mL, Sigma Aldrich), a cell lysis reagent, as described [32 (link)]. Antibodies used are as follows: anti-Oct-4A (2840 Cell Signaling, Milan, Italy), anti-Sox2 (3579 Cell Signaling), anti-KLF4 (4038 Cell Signaling), anti-Nanog (4903 Cell Signaling), anti-c-MYC (5605 Cell Signaling), anti-PD-L1 (13684; Cell Signaling); anti-ALDH3A1 (TA332730, Origene); anti-mPGES1 (160140) and anti-COX-2 (160112, Cayman Chemical, Arcore, Italy); anti-CD133 (PA1217, Boster), anti-NFkB (sc-372, Santa Cruz, CA, USA), and anti-β-actin (Sigma Aldrich). Images were digitalized with CHEMI DOC Quantity One program (Biorad, Milan, Italy
Terzuoli E., Bellan C., Aversa S., Ciccone V., Morbidelli L., Giachetti A., Donnini S, & Ziche M. (2019). ALDH3A1 Overexpression in Melanoma and Lung Tumors Drives Cancer Stem Cell Expansion, Impairing Immune Surveillance through Enhanced PD-L1 Output. Cancers, 11(12), 1963.
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3

Therapeutic Targeting of Metabolic Vulnerabilities in PDAC

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PDAC cells were treated with either 20 mmol/L metformin hydrochloride (PHR 1084; Sigma-Aldrich) or 2 mmol/L 2DG (D8375; Sigma-Aldrich) for 48 or 72 hours. Hypoglycemic PDAC cells were treated with 10 or 20 μmol/L Vorinostat (also known as SAHA, SML0061; Sigma-Aldrich), 50 μmol/L AGN193109 for 48 hours (5758; Tocris, Wiesbaden-Nordenstadt, Germany), or with 10 μmol/L 5-aza-2′-deoxycytidine (Decitabine, A3656; Sigma-Aldrich) for 120 hours.
Jian Z., Cheng T., Zhang Z., Raulefs S., Shi K., Steiger K., Maeritz N., Kleigrewe K., Hofmann T., Benitz S., Bruns P., Lamp D., Jastroch M., Akkan J., Jäger C., Huang P., Nie S., Shen S., Zou X., Ceyhan G.O., Michalski C.W., Friess H., Kleeff J, & Kong B. (2018). Glycemic Variability Promotes Both Local Invasion and Metastatic Colonization by Pancreatic Ductal Adenocarcinoma. Cellular and Molecular Gastroenterology and Hepatology, 6(4), 429-449.
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4

Retinoic Acid Receptor Inhibition in Pregnancy

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The pan retinoic acid receptor inhibitor AGN193109 (Tocris) was administered by oral gavage to pregnant dams at a concentration of 0.8 mg/kg on 3 consecutive days of development (E7.5, E8.5 and E9.5). Stock AGN193109 was resuspended in DMSO (2 mg/mL), stored at −20 °C and freshly diluted in corn oil (1:25) on the first day of administration.
Hauswirth G.M., Garside V.C., Wong L.S., Bildsoe H., Manent J., Chang Y.C., Nefzger C.M., Firas J., Chen J., Rossello F.J., Polo J.M, & McGlinn E. (2022). Breaking constraint of mammalian axial formulae. Nature Communications, 13, 243.
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5

Spatiotemporal modulation of chick embryo

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Chicken embryos were incubated up to stage 4 and RA (Sigma, 400 μM in DMSO) or AGN193109 (Tocris, 100 μM in DMSO) were injected in between the vitelline membrane and the embryo, on top of the PS. Note that lower concentrations did not yield any phenotype. Control embryos were treated with equivalent concentration of DMSO-only. Eggs were sealed with tape and re-incubated for 12h (up to stage 8, before the end of LPM formation). Embryos were then collected on a filter paper ring, washed in PBS solution to stop exposition to RA or AGN193109 and cultured ex ovo in EC culture plates for 24h (up to stage 13, 19-22 somites) or 36h (up to stage 18, 30-36 somites). Embryos were then harvested and fixed in 4% formaldehyde for 2h at room temperature.
Moreau C., Caldarelli P., Rocancourt D., Roussel J., Denans N., Pourquie O, & Gros J. (2019). Timed Collinear Activation of Hox Genes during Gastrulation Controls the Avian Forelimb Position. Current Biology, 29(1), 35-50.e4.
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