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Enzychrom free fatty acid kit

Manufactured by BioAssay Systems
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The EnzyChrom™ free fatty acid kit is a colorimetric assay that quantifies free fatty acid concentrations in biological samples. The kit utilizes an enzymatic reaction to produce a colored product, which is measured spectrophotometrically.

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Lab products found in correlation

Cholestech ldx lipid monitoring system, by Thermo Fisher Scientific (6 mentions) Ultrasensitive insulin elisa kit, by Crystal Chem (4 mentions) Enzychrom triglyceride assay kit, by BioAssay Systems (1 mentions) Alt assay kit, by Cayman Chemical (1 mentions)

6 protocols using enzychrom free fatty acid kit

1

Fasted Blood Metabolic Profiling

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Blood samples were obtained under the fasted condition and glucose level was determined using a Precision QID glucometer (MediSense Inc., Bedford, MA). Serum cholesterol and triglycerides were assayed using Cholestech LDX Lipid monitoring System (Fisher Scientific, Pittsburgh PA). Serum fatty acids were determined using the EnzyChrom™ free fatty acid kit (BioAssay systems, Hayward, CA). Serum fasting insulin was assayed using the Ultra Sensitive Insulin ELISA Kit (Crystal Chem, Inc., Downers Grove, IL, USA). Fasting whole-body insulin sensitivity was estimated with the homeostasis model assessment of insulin resistance (HOMA-IR) according to the formula [fasting plasma glucose (mg/dL) × fasting plasma insulin (μU/mL)]/405.
Lu Z., Zhang X., Li Y., Lopes-Virella M.F, & Huang Y. (2015). TLR4 Antagonist Attenuates Atherogenesis in LDL Receptor-Deficient Mice with Diet-Induced Type 2 Diabetes. Immunobiology, 220(11), 1246-1254.
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2

Comprehensive Metabolic Profiling Protocol

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Blood samples were obtained under the fasted condition, and glucose level was determined using a Precision QID glucometer (MediSense Inc., Bedford, MA, USA). Serum cholesterol was measured using Cholestech LDX Lipid Monitoring System (Fisher Scientific, Pittsburgh, PA, USA). Serum triglycerides were measured using EnzyChrom™ Triglyceride Assay Kit (BioAssay systems, Hayward, CA, USA). Serum FFAs were determined using the EnzyChrom™ free fatty acid kit (BioAssay systems). Serum fasting insulin was assayed using the Ultra Sensitive Insulin ELISA Kit (Crystal Chem, Inc., Downers Grove, IL, USA). Fasting whole-body insulin sensitivity was estimated with the HOMA-IR according to the formula [fasting plasma glucose (mg/dL) × fasting plasma insulin (mU/mL)]/405. ALT was assayed using an ALT Assay Kit (Cayman Chemical, Ann Arbor, MI, USA).
Lu Z., Li Y., Chowdhury N., Yu H., Syn W.K., Lopes-Virella M., Yilmaz Ö, & Huang Y. (2023). The Presence of Periodontitis Exacerbates Non-Alcoholic Fatty Liver Disease via Sphingolipid Metabolism-Associated Insulin Resistance and Hepatic Inflammation in Mice with Metabolic Syndrome. International Journal of Molecular Sciences, 24(9), 8322.
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3

Evaluating Metabolic Biomarkers in Fasted Subjects

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Blood samples were obtained under the fasted condition and glucose level was determined using a Precision QID glucometer (MediSense Inc., Bedford, MA). Serum cholesterol and triglycerides were measured using Cholestech LDX Lipid monitoring System (Fisher Scientific, Pittsburgh PA). Serum free fatty acids (FFAs) were determined using the EnzyChrom™ free fatty acid kit (BioAssay systems, Hayward, CA). Serum fasting insulin was assayed using the Ultra Sensitive Insulin ELISA Kit (Crystal Chem, Inc., Downers Grove, IL). Fasting whole body insulin sensitivity was estimated with the homeostasis model assessment of insulin resistance (HOMA-IR) according to the formula [fasting plasma glucose (mg/dL) × fasting plasma insulin (μU/mL)]/405.
Li Y., Lu Z., Zhang L., Kirkwood C.L., Kirkwood K.L., Lopes-Virella M.F, & Huang Y. (2021). Inhibition of Acid Sphingomyelinase by Imipramine Abolishes the Synergy between Metabolic Syndrome and Periodontitis on Alveolar Bone Loss. Journal of periodontal research, 57(1), 173-185.
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4

Fasted Blood Metabolite Analysis

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Blood samples were obtained under the fasted condition, and glucose level was determined using a Precision QID glucometer (MediSense Inc., Bedford, MA). Serum cholesterol and triglycerides were measured using Cholestech LDX Lipid monitoring System (Fisher Scientific, Pittsburgh, PA). Serum-free fatty acids (FFAs) were determined using the EnzyChrom™ free fatty acid kit (BioAssay Systems, Hayward, CA).
Li Y., Lu Z., Zhang L., Kirkwood K.L., Lopes-Virella M.F, & Huang Y. (2020). Acid sphingomyelinase deficiency exacerbates LPS-induced experimental periodontitis. Oral diseases, 26(3), 637-646.
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5

Metabolic Markers in Fasted Condition

Cited 1 time
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Blood samples were obtained under the fasted condition and glucose level was determined using a Precision QID glucometer (MediSense Inc., Bedford, MA). Serum cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL) and triglycerides were assayed using Cholestech LDX Lipid monitoring System (Fisher Scientific, Pittsburgh PA). Serum fatty acids were determined using the EnzyChrom™ free fatty acid kit (BioAssay systems, Hayward, CA). Serum fasting insulin was assayed using the Ultra Sensitive Insulin ELISA Kit (Crystal Chem, Inc., Downers Grove, IL, USA). Fasting whole-body insulin sensitivity was estimated with the homeostasis model assessment of insulin resistance (HOMA-IR) according to the formula [fasting plasma glucose (mg/dL) x fasting plasma insulin (μU/mL)]/405 31 (link).
Lu Z., Li Y., Brinson C.W., Lopes-Virella M.F, & Huang Y. (2017). Cooperative stimulation of atherogenesis by lipopolysaccharide and palmitic acid-rich high fat diet in low-density lipoprotein receptor-deficient mice. Atherosclerosis, 265, 231-241.
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6

Blood Metabolite Measurement Protocol

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Blood samples were obtained under the fasted condition before the euthanasia and glucose level was determined using a Precision QID glucometer (MediSense Inc., Bedford, MA). Serum cholesterol and triglycerides were measured using Cholestech LDX Lipid monitoring System (Fisher Scientific, Pittsburgh PA). Serum free fatty acids (FFAs) were determined using the EnzyChrom™ free fatty acid kit (BioAssay systems, Hayward, CA).
Li Y., Lu Z., Kirkwood C.L., Kirkwood K.L., Wank S.A., Li A.J., Lopes-Virella M.F, & Huang Y. (2023). GPR40 Deficiency Worsens Metabolic Syndrome-Associated Periodontitis in Mice. Journal of periodontal research, 58(3), 575-587.
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