Anti parp antibody

3T3-L1 cells (ATCC Cat. number CL-173) were purchased from the Bioresource Collection and Research Centre (Food Industry Research and Development Institute, Hsinchu, Taiwan). Anti-β-actin antibodies, 4,6-diamidino-2-phenylindole dihydrochloride (DAPI), dexamethasone (DEX), formaldehyde, insulin, 3-isobutyl-1-methylxanthine (IBMX), Oil Red O, propidium iodide (PI), and Triton X-100 were purchased from Sigma-Aldrich (Missouri, USA). Anti-caspase-3 and anti-PARP antibodies were purchased from Cell Signaling Technology (MA, USA). Fetal bovine serum (FBS) was purchased from Thermo Fisher Scientific Inc. (NY, USA). The PCR Amplification Kit was purchased from the Takara Bio Inc. (Shiga, Japan). Percoll was purchased from GE Healthcare Bio-Sciences AB (Uppsala, Sweden). Trypan blue solution (0.5%) was purchased from BioWest (Nuaille, France). All other reagents were purchased from Invitrogen (CA, USA).

Anti-Caspase-3, Anti-Caspase-8, Anti-Caspase-9, and anti-PARP antibodies for Western blotting were purchased from Cell Signaling Technology (Danvers, MA). Anti-Calreticulin antibody was purchased from Enzo Life Sciences (Farmingdale, NY). Anti-rabbit and anti-mouse HRP conjugated secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA). For cell viability assays, Annexin V FITC/propidium iodide (BD Biosciences) was used following the protocol of the manufacturer.

Anti-S6 ribosomal protein antibodies S6 and pS6, anti-mTOR (raptor and rictor) and anti-PARP antibodies were from Cell Signaling Technology. Anti-β-actin antibody was from Sigma. The goat anti-mouse IgG-HRP, goat anti-rabbit IgG-HRP, goat anti-mouse IgG-biotin and goat anti rabbit-IgG-biotin second antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Vectastain ABC kit and DAB substrate kit were from Vector Laboratories.

AR-42 was a kind gift from Arno Therapeutics, Inc. (Flemington, NJ). Stock solution of AR-42 (100 mM) was prepared in dimethyl sulfoxide (DMSO) (Sigma-Aldrich, St. Louis, MO) and stored at –20°C. For in vivo experiments, AR-42 was prepared as a suspension in a vehicle [10% DMSO, 0.5% methylcellulose (w/v), and 0.1% Tween 80 (v/v) in sterile water] for oral administration to xenograft-bearing athymic nude mice. Anti-cyclin B1 (GNS1), anti-cyclin B2 (H-105), anti-γH₂AX, anti-survivin (D-8), anti-XIAP (A-7), anti-caspase 8, anti-apoptosis inducing factor (AIF) (E1), anti-mouse IgG-CFL 488, anti-p21 (C19), anti-E-cadherin (H108) and anti-p53 (DO-1) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) Anti-caspase 9 (C9), anti-caspase 3, and anti-PARP antibodies were from Cell Signaling Technologies (Beverly, MA). Anti-histone H4 antibody was purchased from Active Motif (Rixensart, Belgium). Anti-N-cadherin was from Genetex (GTX112733, GeneTex Inc., San Antonio, TX).

Mouse anti-caspase-9, Bcl2, Bax, AKT, p-AKT and anti-PARP antibodies were from Cell Signaling Technology. mTOR, p- mTOR, PTEN, p-PTEN, p38, p-p38, CFLAR were from SANTA CRUZ. DR4 and DR5 were from Boster. Rabbit anti ISG12a antibody was from Abcam (ab171919). Mouse anti-β-actin and anti-V5 monoclonal antibody were from Sigma and Invitrogen respectively. Pancaspase inhibitor z-VAD-FMK was obtained from Promega. 2'-O-me-anti-miR-942 (5'-CACAUGGCCAAAACAGAGAAGA-3') & 2'-O-me-GFP miR (5'-AAGGCAAGCUGACCCUGAAGU-3') were from Takara. The Annexin V-FITC apoptosis detection kit and TRAIL were purchased from BD Pharmingen and R&D Systems respectively.