BP scaffolds with/without hMSC seeding were histologically processed using hematoxylin and eosin (H&E), immunohistochemistry, and immunofluorescence double staining. Briefly, the scaffolds were fixed in 10% buffered formalin, embedded in paraffin, and processed for staining. Laminin immunohistochemistry was performed using anti-laminin primary antibody (1:50, Abcam, Cambridge, MA) and horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody (DAKO, Carpinteria, CA). Immunofluorescence double staining was performed using mouse anti-eGFP (1:200, Abcam, Cambridge, MA) and rabbit anti-laminin primary antibodies. Fluorescent anti-mouse secondary antibody and anti-rabbit secondary antibody tagged with Alexa Fluor 405 (402Ex/421Em) and Alexa Fluor 647 (652Ex/668Em) (1:500, Abcam), respectively, were used for visualization. For both stains, slides were imaged using a Nikon Eclipse E600 microscopy and digital images collected.
Anti laminin primary antibody
Manufactured by Abcam
Sourced in United States
The Anti-laminin primary antibody is a research-grade reagent used for the detection and identification of laminin in various applications, such as immunohistochemistry and Western blotting. Laminin is a major structural component of the basement membrane and plays a crucial role in cellular adhesion and migration. This antibody provides a reliable and specific tool for researchers studying laminin and its functions in biological systems.
Automatically generated - may contain errors
2 protocols using anti laminin primary antibody
1
Histological Analysis of BP Scaffolds
2
Immunofluorescence Analysis of Muscle Fibers
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TA muscles were fixed in 4% (v/v) paraformaldehyde and permeabilized with 0.2% Triton X-100 in phosphate-buffered saline to determine the skeletal muscle fibers' cross-sectional areas. The muscles were incubated sequentially with an anti-laminin primary antibody (1:50, Abcam, Cambridge, MA, USA) and goat anti-rabbit Alexa Flour 488 conjugated secondary antibody (1:500, Abcam), protected from light. The sections were individually mounted in DAPI (Abcam) and sealed with a coverslip. The specimens were analyzed using a confocal laser-scanning microscope (Nikon Corporation, Tokyo, Japan). DAPI (blue) and laminin (green) fluorescence were excited with a 405 nm and a 488 nm, respectively.
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