Pentobarbital sodium

Manufactured by Kyoritsu Seiyaku

Sourced in Japan

Pentobarbital sodium is a barbiturate compound. It is commonly used as a sedative-hypnotic medication and has various laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

3 aminopropyltriethoxysilane, by Shin-Etsu Chemical (4 mentions) Isoflurane, by Fujifilm (4 mentions) Cm3000, by Leica Microsystems (3 mentions) Elisa, by Fortis Life Sciences (3 mentions) Lsm 510 meta, by Zeiss (2 mentions) Isoflurane, by Pfizer (2 mentions) Paraformaldehyde (pfa), by Fujifilm (2 mentions) Butorphanol, by Meiji Seika Pharma (2 mentions) Cuy650p5, by Nepa Gene (2 mentions) Bx53f, by Olympus (2 mentions) High fat diet (hfd), by Research Diets (1 mentions) Isoflurane, by Abbott (1 mentions) La theta lct 100, by Hitachi (1 mentions) Xylocaine cartridge for dental use, by Dentsply (1 mentions) Anti cleaved caspase 3 antibody, by Santa Cruz Biotechnology (1 mentions) Apotag peroxidase in situ apoptosis detection kit, by Merck Group (1 mentions) Deadend fluorometric tunel system, by Promega (1 mentions) Anti erk1 2 antibody, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)

100 protocols using pentobarbital sodium

Subcutaneous Transplantation of iPS-MSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The animal experiments in this study were approved by the Animal Research and Care Committee of our institution. Mouse iPS-MSCs and mouse BMSCs were suspended in alginate gel at the desired concentration (1×10 6 cells per 40 μl alginate bead). 36 male nude mice (Six-week-old, BALB/cScl-nu/nu, Japan SLC) were used for the transplantation.
Before surgery, the mice were given sufficient anesthesia by intraperitoneal administration of pentobarbital sodium (Kyoritsu Seiyaku, Tokyo, Japan) 15 times diluted by physiological saline (10 mL/kg; Otsuka Pharmaceutical, Tokyo, Japan). Each alginate gel bead containing cells was transplanted subcutaneously into the dorsal flank of nude mice.

Hontani K., Onodera T., Terashima M., Momma D., Matsuoka M., Baba R., Joutoku Z., Matsubara S., Homan K., Hishimura R., Xu L, & Iwasaki N. (2019). Chondrogenic differentiation of mouse induced pluripotent stem cells using the three-dimensional culture with ultra-purified alginate gel. Journal of biomedical materials research. Part A, 107(5).

+ Open protocol

+ Expand

Exercise, Melatonin, and High-Fat Diet Effects

Check if the same lab product or an alternative is used in the 5 most similar protocols

Male Wistar rats (4 weeks old: SLC) were housed in a room at 23°C with a 12:12‐hour light‐dark cycle. All animals were divided into five groups (four rats in each group): normal diet (ND)‐fed sedentary (ND‐SED), HFD‐fed sedentary (HFD‐SED), HFD‐fed ET (HFD‐ET), HFD‐fed MT (HFD‐MT), and HFD‐fed ET plus MT (HFD‐ETMT) group. ND‐SED rats were fed a standard diet (MF, Oriental Yeast), and the rats in the HFD group were fed HFD (60% fat, Research Diets) for 17 weeks. Water and food were available ad libitum.
Exercise training and MT were started 8 weeks after the beginning of dietary intervention. HFD‐ET and HFD‐ETMT rats ran on a treadmill (5‐degree incline), 5 d/wk, for 9 weeks according to a protocol reported.¹⁰ The running time and speed were increased progressively until after 6 weeks, when the rats ran continuously for 90 minutes at 30 m/min. HFD‐MT and HFD‐ETMT rats received an intraperitoneal injection of MT at 5 mg/kg body weight for 9 weeks. The dose of melatonin administered was based on previous studies.⁷, ¹¹ Following all interventions, the rats were euthanized with pentobarbital sodium (0.5 mg/kg body weight, i.p.; Kyoritsu Seiyaku), and the cerebellum was removed. HFD‐ET and HFD‐ETMT rats were euthanized at least 36 hours after the last exercise session. All experiments were approved by the Animal‐Care Committee of Doshisha University.

Sugiyama A., Kato H., Takakura H., Osawa S., Maeda Y, & Izawa T. (2020). Effects of physical activity and melatonin on brain‐derived neurotrophic factor and cytokine expression in the cerebellum of high‐fat diet‐fed rats. Neuropsychopharmacology Reports, 40(3), 291-296.

+ Open protocol

+ Expand

Periodontal Defect Repair in Nude Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols

All study protocols and procedures were approved by the Animal Care Ethics Committee of Tokyo Medical and Dental University. The periodontal defects were surgically created as described previously with minor modification [24 (link),25 (link),26 (link)]. Briefly, a total of 22 (n = 10 regeneration, n = 4 cell labeling, n = 6 human Alu PCR, n = 2 dead during surgery) male athymic nude rats (F344/N-Jcl-rnu/rnu; age 7–8 weeks) were anesthetized using isoflurane (Abbott Laboratories, Queenborough, UK) and pentobarbital sodium (Kyoritsu Seiyaku, Tokyo, Japan). An extraoral incision was made at the bottom of the mandible and the buccal plate was exposed (Figure 1E). The buccal bone, PDL, cementum, and dentin from the mesial root of the mandibular first molar to the mesial root of the second molar were carefully removed to create a periodontal defect using rotatory instruments (Figure 1F). Each periodontal defect in the buccal area was 2 mm in height and 3 mm in width. After creation of the defect, PDLSC-amnion (experimental) or amnion (control) was trimmed and placed to cover the periodontal defect and the masseter and skin were sutured with 7-0 or 5-0 silk (Mani, Tochigi, Japan). The decision of treatment in each experiment was made randomly by a third person blinded to the treatment allocation after the periodontal defect had been created.

Iwasaki K., Akazawa K., Nagata M., Komaki M., Honda I., Morioka C., Yokoyama N., Ayame H., Yamaki K., Tanaka Y., Kimura T., Kishida A., Watabe T, & Morita I. (2019). The Fate of Transplanted Periodontal Ligament Stem Cells in Surgically Created Periodontal Defects in Rats. International Journal of Molecular Sciences, 20(1), 192.

+ Open protocol

+ Expand

Abdominal Fat Composition Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

At the end of the experiment, mice were fasted for 12 h and anesthetized using intraperitoneal injections of pentobarbital sodium (Kyoritsuseiyaku Co., Tokyo, Japan).
The mouse mode was used for CT scanning (La Theta LCT100, Hitachi Aloka Medical, Ltd., Tokyo, Japan). Abdominal compositions were estimated from images of fat slices that were acquired at 2-mm intervals between the second and fourth lumbar vertebrae using the La Theta software (version 2.10).

Iizuka Y., Kim H., Izawa T., Sakurai K., Hirako S., Wada M, & Matsumoto A. (2016). Protective effects of fish oil and pioglitazone on pancreatic tissue in obese KK mice with type 2 diabetes. Prostaglandins, leukotrienes, and essential fatty acids, 115.

+ Open protocol

+ Expand

Rat Intestinal Tissue Preparation for TLR and RANK Detection

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

After euthanasia with an overdose peritoneal injection of pentobarbital sodium (Kyoritsu Seiyaku, Tokyo, Japan), small tissue blocks with Peyer’s patches were removed from the ileum. For the detection of TLR-2, -4 or -9, the
tissue blocks from 10 rats were immersion-fixed in 4.0% paraformaldehyde fixative in phosphate buffer (PB; pH 7.4) for 24 hr at 4°C. For the detection of RANK, RANKL or cleaved caspase-3, the tissue blocks from another 10 rats
were immersion-fixed in 4.0% paraformaldehyde fixative in PB for 1 hr at 4°C. Then, all tissue blocks were snap-frozen in liquid nitrogen with reference to the embedding method described previously [28 (link)]. Four micrometer-thick sections were cut using a Coldtome CM1950 (Leica Biosystems, Nussloch, Germany) and were placed on slide glasses precoated with 2% 3-aminopropyltriethoxysilane (Shin-Etsu Chemical, Tokyo,
Japan) and stored at −30°C until use.

YUASA H., MANTANI Y., MASUDA N., NISHIDA M., ARAI M., YOKOYAMA T., TSURUTA H., KAWANO J., HOSHI N, & KITAGAWA H. (2017). Mechanism of M-cell differentiation accelerated by proliferation of indigenous bacteria in rat Peyer’s patches. The Journal of Veterinary Medical Science, 79(11), 1826-1835.

+ Open protocol

+ Expand

Anesthesia and Submandibular Gland Dissection in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

General anesthesia was induced in the mice by intraperitoneal injections of pentobarbital sodium (40 mg/kg body weight, Kyoritsu Seiyaku Corporation, Tokyo, Japan), followed by infiltration anesthesia with 0.3 ml lidocaine containing 2% epinephrine and sodium chloride (Xylocaine Cartridge for Dental Use, DENTSPLY International, Tokyo, Japan) subcutaneously administered to the median cervix, and then a skin incision was made.
Then, the both of submandibular glands were dissected from the surrounding tissue, and the both of submandibular glands were cutted in the shift portion of the main excretory duct.

Kuraji M., Matsuno T, & Satoh T. (2016). Astaxanthin affects oxidative stress and hyposalivation in aging mice. Journal of Clinical Biochemistry and Nutrition, 59(2), 79-85.

+ Open protocol

+ Expand

Circadian Clock Gene Rhythmicity in Rat Adipose Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Four-week-old male Wistar rats (n = 24, SLC, Shizuoka, Japan) were housed in a temperature-controlled room at 23 °C with a 12:12 h light-dark cycle. Food and water were available ad libitum. All rats were allowed seven days to acclimatize to their new environment prior to establishing a normal light/dark cycle: ZT0 (08:00) indicates lights on, and ZT12 (20:00) indicates lights off. To confirm circadian clock gene rhythmicity in adipose tissue, Bmal1 and Per2 expression levels were measured every 3 h over a 24-h period (eight time points) in epididymal adipose tissue collected from 5-week-old rats. The animals were anaesthetized with an intraperitoneal injection of pentobarbital sodium (5 mg/100 g body weight; Kyoritsu Seiyaku, Tokyo, Japan) and killed by exsanguination through the abdominal aorta at ZT0, 3, 6, 9, 12, 15, 18, and 21 (n = 3 each). Epididymal adipose tissue was rapidly removed, immediately frozen in liquid nitrogen, and stored at −80 °C until RNA preparation. All animal protocols were approved by the Animal Care Committee of Doshisha University (A15010, A19005).

Kato H., Ogasawara J., Takakura H., Shirato K., Sakurai T., Kizaki T, & Izawa T. (2020). Exercise Training-Enhanced Lipolytic Potency to Catecholamine Depends on the Time of the Day. International Journal of Molecular Sciences, 21(18), 6920.

+ Open protocol

+ Expand

Immunolabeling of Mouse Vaginal Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice under anesthesia with pentobarbital sodium (0.648 mg/10 g body weight via intraperitoneal injection; Kyoritsuseiyaku Co., Tokyo, Japan) were subjected to transcardiac perfusion of 4% paraformaldehyde. The vaginas were excised from the mice and fixed overnight in 4% paraformaldehyde solution. The vaginas were embedded longitudinally in paraffin and cut into 4-μm serial sections. The sections were immunolabeled with anti-mouse Sema4D (cat.no D142-3; Medical and Biological Laboratories Co., Ltd., Nagoya, Japan), anti-plexin-B1 (cat.no. sc-25642; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and anti-cleaved caspase-3 antibody (cat.no. #9664; Cell Signaling Technology, Beverly, MA, USA). TUNEL assay was performed, as described previously (18 (link)), using a Dead End™ Fluorometric TUNEL system (Promega, Madison, WI, USA) and an ApoTag Peroxidase In Situ Apoptosis Detection kit (Chemicon International, Inc., Temecula, CA, USA) according to the manufacturer’s instructions.

ITO T., BAI T., TANAKA T., YOSHIDA K., UEYAMA T., MIYAJIMA M., NEGISHI T., KAWASAKI T., TAKAMATSU H., KIKUTANI H., KUMANOGOH A, & YUKAWA K. (2014). Semaphorin 4D induces vaginal epithelial cell apoptosis to control mouse postnatal vaginal tissue remodeling. Molecular Medicine Reports, 11(2), 829-836.

+ Open protocol

+ Expand

Intratracheal Instillation of MWCNTs in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols

MWCNTs were suspended in 0.4 ml of 0.5 mg ml⁻¹ Triton‐X aqueous solution at concentrations of 0.50 and 1.38 mg ml⁻¹. These were intratracheally instilled into male Wistar rats, at a dose of 0.20 or 0.55 mg per rat using tip cutting feeding needle for rat (Cat No. 7204; Fuchigami Kikai Co., Kyoto, Japan). The rats were 8 weeks old with a mean body weight of 196 g (range 186–207) and had been anesthetized by inhalation of sevoflurane (Maruishi Pharmaceutical Co., Ltd., Osaka, Japan). An aqueous solution of 0.5 mg ml⁻¹ Triton‐X was intratracheally instilled into the control rats. In each of the above 3 groups, 11 rats were anesthetized with an intraperitoneal injection of pentobarbital sodium (Kyoritsu Seiyaku Corporation, Japan) on days 1, 3, 7, 28, 91, 175, and 364 after instillation, and dissection and gross autopsy were performed. Five rats in each group were used for the MWCNTs analysis, 5 rats in each group were used for the optical microscope observations, and 1 rat was used for TEM observation.
All animal handling procedures were conducted in accordance with the guidelines in the Japanese Guide for the Care and Use of Laboratory Animals, as approved by the Animal Care and Use Committee, University of Occupational and Environmental Health, Japan, or by the Institutional Animal Care and Use Committee, National Institute of Advanced Industrial Science and Technology.

Shinohara N., Nakazato T., Ohkawa K., Tamura M., Kobayashi N., Morimoto Y., Oyabu T., Myojo T., Shimada M., Yamamoto K., Tao H., Ema M., Naya M, & Nakanishi J. (2015). Long‐term retention of pristine multi‐walled carbon nanotubes in rat lungs after intratracheal instillation. Journal of Applied Toxicology, 36(4), 501-509.

+ Open protocol

+ Expand

Histological Analysis of Tissue Implants

Check if the same lab product or an alternative is used in the 5 most similar protocols

Histology was performed according to previous reports (Maekawa et al., 2005 ▶ ; Matsumoto et al., 2011 ▶ ). Four weeks after implantation, animals were deeply anesthetized with pentobarbital sodium (Kyoritsu Seiyaku Co., Tokyo, Japan) by intraperitoneal injection and perfused with 4% paraformaldehyde dissolved in phosphate buffered saline. Fixed tissue was cryoprotected in 20% sucrose in phosphate buffered saline, embedded and frozen at 193 K in optimum cutting temperature compound (Sakura Finetechnical, Tokyo, Japan), and cryosectioned (25 µm thick). Frozen sections were washed with Tris-based saline containing 0.1% Tween-20. Images of prepared sections were obtained with a microscope (LSM510 Meta; Carl Zeiss, Jena, Germany).

Takashima K., Hoshino M., Uesugi K., Yagi N., Matsuda S., Nakahira A., Osumi N., Kohzuki M, & Onodera H. (2015). X-ray phase-contrast computed tomography visualizes the microstructure and degradation profile of implanted biodegradable scaffolds after spinal cord injury. Journal of Synchrotron Radiation, 22(Pt 1), 136-142.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!