Sps 4

Manufactured by Agilent Technologies

Sourced in United States

The SPS 4 is a lab equipment product from Agilent Technologies. It is a sample preparation system designed for automated processing of samples prior to analysis. The core function of the SPS 4 is to provide a standardized and consistent sample preparation workflow to ensure reliable and reproducible analytical results.

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Lab products found in correlation

5100 icp oes, by Agilent Technologies (2 mentions) Icp standard 4, by Merck Group (2 mentions) Agilent 7800 icp ms, by Agilent Technologies (1 mentions) 7800 icp ms, by Agilent Technologies (1 mentions) Masshunter 4, by Agilent Technologies (1 mentions) Infinity 2, by Agilent Technologies (1 mentions)

6 protocols using sps 4

Quantification of Trace Elements in Vaginal Fluid

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The zinc and copper concentrations of the pre-treated fluid samples were measured by inductively coupled plasma optical emission spectrometry (ICP-OES 5100, Agilent Technologies, USA). The measurements were conducted in SVDV (Synchronous Vertical Dual View) mode, gaining intensity data from the axial and radial view, simultaneously. We applied an automatic sample introduction (SPS 4, Agilent Technologies, USA), and measured the samples in a randomized design. We performed measurements to generate a five-point calibration curve for the quantitative analysis of copper and zinc. Calibration solutions were diluted from a multi-element standard of 1000 mg/L (ICP standard IV, Merck, Germany) with 0.1 M nitric acid in ultrapure water. We expressed the trace element concentration of vaginal fluid samples in mg/L. The operating parameters of ICP-OES are described in Table 1.Table 1

The operating parameters of ICP-OES 5100 (Agilent Technologies)

ICP-OES operating parameters
Repetition	3
Pump speed	15 rpm
Uptake time	20 s, fast pump
Rinse time	30 s, fast pump
Read time	10 s
RF energy	1.20 kW
Stabilization time	7 s
View mode	SVDV
view height	0 mm
Nebulizer gas	0.70 l/min
Plasma gas	12.0 l/min

Sipos A.G., Pákozdy K., Jäger S., Larson K., Takacs P, & Kozma B. (2021). Fractional CO2 laser treatment effect on cervicovaginal lavage zinc and copper levels: a prospective cohort study. BMC Women's Health, 21, 235.

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Trace Element Analysis of Tissue and Blood

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The elemental analysis of the pre-treated tissue and blood samples was carried out by inductively coupled plasma optical emission spectrometry (ICP-OES 5100, Agilent Technologies, Santa Clara, CA, USA). The measurements were conducted in SVDV (Synchronous Vertical Dual View) mode, gaining intensity data from the axial and radial view, simultaneously. An automatic sample introduction was applied (SPS 4, Agilent Technologies, Santa Clara, CA, USA), and the samples were measured in a randomized design [20 (link)]. We have performed measurements to generate a five-point calibration curve for the quantitative analysis of copper and zinc. We have diluted the calibration solutions from a multi-element standard of 1000 mg/l (ICP standard IV, Merck, Germany) with 0.1 M nitric acid in ultrapure water. We have expressed trace element concentration of vaginal tissues in milligrams per kilogram (mg/kg), and trace element concentration of whole blood samples in milligrams per liter (mg/l) [20 (link)].

Csikós A., Kozma B., Baranyai E., Miklós I., Larson K., Póka R, & Takacs P. (2021). Evaluation of zinc and copper levels in vaginal tissues and whole blood: correlation with age. BMC Women's Health, 21, 62.

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ICP-MS Analysis of Trace Elements

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For small volume LC analysis (Agilent Infinity II coupled to Agilent 7800 ICP-MS), the following instrument parameters were used: RF power 1550 V, RF matching 1.00 V, nebulizer gas 0.99 L/min, option gas 0.0%, nebulizer pump 0.30 rps, S/C temp 2 °C, makeup gas 0.00 L/min, extract 1 lens 0.0 V, extract 2 lens −195.0 V, omega bias −95 V, omega lens 8.4 V, cell entrance −40 V, cell exit −60 V, deflect 0 V, plate bias −55 V, He flow rate 4.0 mL/min, octupole bias −18 V, octupole RF 200 V, and energy discrimination 3.0 V.
For large volume analysis (Agilent SPS 4 coupled to Agilent 7800 ICP-MS), the following instrument parameters were used: RF power 1550, RF matching 1 V, nebulizer gas 1.00 L/min, option gas, 0.0 %, nebulizer pump 0.10 rps, S/C temp 2 °C, makeup gas 0.00 L/min, extract 1 lens 0.0 V, extract 2 lens −200.0 V, omega bias −105 V, omega lens 7.5 V, cell entrance −40, cell exit −60 V, deflect −0.6 V, plate bias −55 V, He flow rate 4.5 mL/min, octupole bias −18.0 V, octupole RF 200 V, and energy discrimination 5.0 V.

Larson J., Tokmina-Lukaszewska M., Fausset H., Spurzem S., Cox S., Cooper G., Copié V, & Bothner B. (2023). Arsenic Exposure Causes Global Changes in the Metalloproteome of Escherichia coli. Microorganisms, 11(2), 382.

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Quantitation of Trace Metals in Biomass

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Biologic replicates were diluted to the same protein concentration. Then equal parts were pooled to create one sample per condition. The metals were liberated from biologic molecules using a 5% nitric acid digestion. The precipitate was pelleted via centrifugation, and the supernatant was collected for analysis. Metal analysis was performed using an Agilent Infinity II autosampler coupled to an Agilent 7800 ICP-MS. The mobile phase was 2% HNO₃, 0.5% HCl in water, with a flow rate of 1 mL/min. The signals of five metal ions were monitored for 30 s, and included: ²⁴Mg, ⁵⁶Fe, ⁶³Cu, ⁶⁶Zn, and ⁷⁵As, with an integration time/mass of 0.5 s per analyte. The quantification of analytes was performed in Agilent MassHunter 4.6 (version C.01.06) against a standard curve. Media with and without arsenic was digested in the same fashion The metals of interest were analyzed using an Agilent SPS 4 autosampler coupled to an Agilent 7800 ICP-MS in helium mode. The quantification of analytes was performed against a standard curve in MassHunter.

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Elemental Composition Analysis by ICP-OES

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Concentrations of 24 elements were determined by an Agilent ICP OES system (5100 SVDV model, Agilent Technologies, Santa Clara, USA) as described previously [31 (link),32 (link)]. An auto sampler (Agilent SPS4), a Meinhard type nebulizer and a double pass spray chamber was employed to introduce the sample solutions. Argon gas was used to supply the plasma and nitrogen gas was used for sample introduction and optical purge. Standard solutions of As and tin (Sn) were prepared from mono element spectroscopic standards with a concentration of 1000 mg/l. Calibration series of other elements including silver (Ag), aluminum (Al), B, bismuth (Bi), Ca, Cd, Co, Cr, Cu, iron (Fe), gallium (Ga), indium (In), K, lithium (Li), Mg, manganese (Mn), Na, Ni, Pb, strontium (Sr), thallium (Tl), and zinc (Zn) were prepared from a multi element spectroscopic standard solution with element concentrations of 1000 mg/l. In both cases, a 5-point calibration curve was used. The standard solutions were diluted with 0.1 M HNO₃ prepared in ultrapure water. Blank samples were used to check the purity of water and glassware applied. Three parallel measurements were performed for each sample. Operating conditions of the ICP-OES are shown in Supplement 1.

Muhollari T., Szűcs S., Sajtos Z., McKee M., Baranyai E., Ádány R, & Pál L. (2023). Heavy metals in unrecorded Albanian rakia: A pilot study on a potential public health risk. Heliyon, 9(3), e13717.

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Inductively Coupled Plasma Mass Spectrometry of Nanoparticles

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In order to determine the mass concentrations and the present element ratios of the particle dispersions inductively coupled plasma mass spectrometry measurements were done using an Agilent 8900 mass spectrometer coupled to an autosampler SPS 4 by Agilent Technologies with the software MassHunter. For this, different aliquots of the nanoparticle samples were taken and the solvent was evaporated. The resulting precipitate was subsequently dissolved in nitric acid, further diluted with Milli-Q water in order to reach suitable mass concentrations for the respective measurement and finally measured against calibration standard solutions with known mass concentrations using a nebuliser type MicroMist. The Ni measurements were done in a collision cell using the gas modus with a helium gas flux of 1 mL min -1 due to the high concentrations while P was measured in normal mode.

Himstedt R ., Hinrichs D ., Sann J ., Weller A ., Steinhauser G , & Dorfs D . (2019). Halide ion influence on the formation of nickel nanoparticles and their conversion into hollow nickel phosphide and sulphide nanocrystals. Nanoscale, 11(32).

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