Human lung cancer cell line A549, MCF-7, PC3 and L-132 were obtained from National Centre for Cell Science (NCCS, Pune, India). A549, MCF-7, PC3 and L-132 were maintained in Ham’s F12 and DMEM medium (Invitrogen, USA) respectively. The media was supplemented with 10% FBS and 1X antibiotics (Invitrogen, USA) in a humidified atmosphere with 5% CO2.
L 132
Manufactured by Thermo Fisher Scientific
Sourced in United States, India
The L-132 is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor configuration and can accommodate a variety of sample tube sizes. The centrifuge is capable of reaching a maximum speed of 6,000 rpm and generates a maximum relative centrifugal force (RCF) of 4,300 x g. The L-132 is a compact and durable instrument suitable for use in various laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Ham s f12, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by National Centre for Cell Science (1 mentions)
L 132, by National Centre for Cell Science (1 mentions)
Rpmi 1640, by HiMedia (1 mentions)
2 protocols using l 132
1
Cell Line Maintenance Protocol
2
Cell Culture Protocol for Lung Cancer Cell Lines
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A549, L132, NCI-H460, NCI-H26 were purchased from National Center for Cell Science, Pune, India. A549 and L132 cells were cultured in Ham’s F12 and DMEM medium respectively (GIBCO, Invitrogen). NCI-H460 and NCI-H26 cells were cultured in RPMI-1640 (Himedia, India). The medium contained 10% FBS (GIBCO, Invitrogen), 1× Antibioic-Antimycotic solution (GIBCO, Invitrogen). All the cells were maintained in humidified condition with 5% CO2 at 37 °C.
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