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Goat anti rabbit or goat anti mouse secondary antibody

Manufactured by Proteintech
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Sourced in Japan, United States

The Goat anti-rabbit or goat anti-mouse secondary antibody is a laboratory reagent used in various immunological techniques. It is designed to detect and bind to primary antibodies raised in rabbit or mouse, respectively. This secondary antibody is typically conjugated with a reporter molecule, such as a fluorescent dye or enzyme, to facilitate visualization or signal amplification in downstream applications.

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Lab products found in correlation

Anti spc, by Proteintech (1 mentions) Anti aqp5, by Proteintech (1 mentions) Anti β catenin, by Proteintech (1 mentions) Anti sox9, by Santa Cruz Biotechnology (1 mentions) Bca kit, by Solarbio (1 mentions) Gtse1, by Proteintech (1 mentions) Gapdh, by Proteintech (1 mentions)

3 protocols using goat anti rabbit or goat anti mouse secondary antibody

1

Immunofluorescence Analysis of Lung Cells

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The lung paraffin sections were dewaxed, hydrated, antigen-repaired, and circled. The paraffin-embedded sections or primary cell slides were sealed with 10% goat serum at room temperature (22–24 °C) for 1 h, then away from light at 4 °C overnight with antibodies:anti-sox9 (1:100, Santa Cruz), anti-β-catenin (1:200, Proteintech), anti-SPC (1:100, Proteintech), and anti-AQP5 (1:100, Proteintech). Next, the goat anti-rabbit or goat anti-mouse secondary antibody (1:200, Proteintech) was used to incubate for 2 h. After DAPI was used to stain the cell nucleus, a fluorescence microscope (E800, Nikon, Japan) was used to observe protein expression.
Wu D., Bai D., Yang M., Wu B, & Xu W. (2024). Role of Sox9 in BPD and its effects on the Wnt/β-catenin pathway and AEC-II differentiation. Cell Death Discovery, 10, 20.
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2

Western Blot Analysis of Hepatic Proteins

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Hepatic tissue and cellular proteins were extracted according to the RIPA instructions (Solarbio, Beijing, China), and the proteins concentration were determined using the BCA kit (Solarbio, Beijing, China). 10% or 12% SDS-PAGE were used to separated proteins, then transferred to PVDF membrane, blocked with 5% skim milk powder for 1 h. Primary antibody was added and incubated overnight at 4 °C, followed by incubation with goat anti-rabbit or goat anti-mouse secondary antibody (Proteintech, Wuhan, China) at room temperature for 1 h. The gel imaging system (Cytiva, America) was used for color development after the addition of ECL luminescent solution (NCM Biotech, Suzhou, China). The primary antibodies used for western blot are shown in Table 1. (Original protein bands are provided in the Supplementary file).

Antibody information for westrn blot.

AntibodyCompanyCatalog numberSourceConcentration
Cleaved caspase 3CST9664Rabbit1:1000
BAXProteintech50599-2-IgRabbit1:1000
BCL-2HUABIOET1702-53Rabbit1:1000
p-p65CST3033Rabbit1:1000
p65Proteintech10745-1-APRabbit1:1000
p-IKKβCST2697Rabbit1:1000
IKKβProteintech15649-1-APRabbit1:600
p-IκBαCST2859Rabbit1:1000
p-p38CST4511Rabbit1:1000
p38HUABIOET1702-65Rabbit1:1000
p-JNKCST4668Rabbit1:1000
JNKProteintech24164-1-APRabbit1:1000
MKP5Santa Cruzsc-374276Mouse1:1000
GAPDHProteintech60004-1-IgMouse1:5000
Yu Q., Li J., Cui M., Mei C., He Q, & Du X. (2024). 6-Gingerol attenuates hepatic ischemia/reperfusion injury through regulating MKP5-mediated P38/JNK pathway. Scientific Reports, 14, 7747.
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3

Western Blot Analysis of Protein Expression

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Total protein of cells was extracted with a radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor under the condition of an ice bath. The extracted protein and loading buffer were added and transferred onto 0.22-mm methanol-excited polyvinylidene fluoride membranes after SDS-PAGE electrophoresis, and then membranes were blocked with 5% nonfat milk. Next, we incubated membranes with the following primary antibodies at 4°C overnight: TAF15 (1:1,000) (Cell Signaling Technology, USA), MTF1 (1:1,000) (Proteintech, USA), YY2 (1:200) (Santa Cruz Biotechnology, USA), GTSE1 (1:1,000) (Proteintech, USA), and GAPDH (1:10,000) (Proteintech, USA), followed by incubation with related horseradish peroxidase (HRP)-conjugated secondary antibodies, including goat anti-rabbit or goat anti-mouse secondary antibody (1:10,000) (Proteintech, USA) at room temperature for 1.5 h. The relative integrated density values (IDVs) were observed based on GAPDH as a control.
Ruan X., Zheng J., Liu X., Liu Y., Liu L., Ma J., He Q., Yang C., Wang D., Cai H., Li Z., Liu J, & Xue Y. (2020). lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation. Molecular Therapy. Nucleic Acids, 20, 823-840.
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