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2 protocols using hyaluronic acid 4

1

Antioxidant Assays and Phytochemical Analysis

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Nitrotetrazolium blue chloride (NBT), xanthine, xanthine oxidase, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), potassium persulfate, 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (ferrozine), iron (II) chloride tetrahydrate (FeCl2 × 4H2O, 1,3,5-Tri(2-pyridyl)-2,4,6-triazine (TPTZ), iron (III) chloride (FeCl3), aluminum chloride (AlCl3), potassium acetate, Folin−Ciocalteu reagent, sodium nitrite, sodium molybdate, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), ascorbic acid, 2(3)-t-Butylhydroquinone monomethyl ether (BHA), 2(3)-t-Butyl-4-hydroxyanisole, hyaluronic acid (IV), escin, hyaluronidase from bovine testes, hexadecyltrimethylammonium bromide (CTAB), L-tyrosine, koji acid, and tyrosine from mushrooms were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). The standards of eleutheroside B ≥98.0% (HPLC), eleutheroside E ≥98.0% (HPLC), eleutheroside E1 ≥98.0% (HPLC), protocatechuic acid ≥97%, p-hydroxybenzoic acid 99%, vanillic acid ≥97%, caffeic acid ≥98%, and ferulic acid ≥99% were also purchased from Sigma-Aldrich. Solvents used for extraction were purchased from Avantor Performance Materials (Gliwice, Poland).
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2

Antioxidant and Enzyme Inhibition Assays

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1,3,5-Tri(2-pyridyl)-2,4,6-triazine (TPTZ), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (ferrozine), Folin−Ciocalteu reagent ascorbic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2(3)-t-Butyl-4-hydroxyanisole, 2(3)-t-Butylhydroquinone monomethyl ether (BHA), escin, donepezil, koji acid, iron(II) chloride tetrahydrate (FeCl2 × 4H2O, iron(III) chloride (FeCl3), potassium persulfate, buffers for enzymatic analysis, L-tyrosine, L-DOPA, tyrosine from mushrooms, acetylcholine (ACh), acetylcholinesterase, 5,5′-Dithiobis(2-nitrobenzoic acid) (DTNB), hyaluronidase from bovine testes, hyaluronic acid (IV), and hexadecyltrimethylammonium bromide (CTAB) were purchased from Sigma-Aldrich Corp (Saint Louis, MO, USA). Solvents used for extraction were purchased from Avantor Performance Materials, Poland S.A. (Gliwice, Poland).
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