Fitc anti rabbit igg antibody

Annexin-V-FITC, FITC-anti-Rabbit IgG antibody, and PE-anti-Mouse IgG were purchased from Biolegend (San Diego, CA) and used for immunocytochemistry and flow cytometry analysis. Mouse anti-FLAG antibody (F1804) was obtained from Sigma. Anti-mouse secondary antibodies, AlexaFluor conjugated-far-red 647 (594/633 nm), and -yellow 532 (532/554 nm) were obtained from Invitrogen. Anti-β-actin antibody (60008–1) and anti-GAPDH-HRP (HRP-60004) were purchased from Proteintech (Chicago, IL). Antibody against IDH2 (ab55271) was purchased from Abcam (Cambridge, MA). Antibody against STAT3 (sc-482) was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). Antibodies against phospho-STAT3-Y705 (9145S), FAK (3285-P) and phospho-FAK-Y397 (8556P) were purchased from Cell Signaling (Danvers, MA). Antibody against cMyc (LT0421) was purchased from Lifetein (South Plainfield, NJ) and anti-V5 antibody (600–401–378) from Rockland Immunochemicals (Pottstown, PA). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and anti-mouse antibodies were purchased from Invitrogen (Carlsbad, CA). Temozolomide, cisplatin, bortezomib, and vincristine were purchased from Millipore Sigma. Lyophilized (R)-2-HG and (S)-2-HG were generously provided by Dr. Ryan Looper (University of Utah). (R)-2-HG-TFMB was also synthesized by the Organic Synthesis Lab of FCCC.