CD8 T cells were magnetically purified (BioLegend) from OT1 mice to > 95% purity. Cells were counted and 200–500 (as indicated) OT1s were transferred to recipient mice by tail vein injection 1 day prior to immunization. CD4 T cell-depletion was achieved by IV injection of 50 μg αCD4 antibody (clone GK1.5, BioXCell) 2–5 days prior to immunization.
αcd4 antibody clone gk1
Manufactured by BioXCell
The αCD4 antibody (clone GK1.5) is a monoclonal antibody that specifically binds to the CD4 receptor on T cells. This antibody is a laboratory tool used for the identification and isolation of CD4+ T cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using αcd4 antibody clone gk1
1
OT1 Cell Adoptive Transfer
2
OT1 Cell Adoptive Transfer
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