Ab108411

Manufactured by Abcam

Sourced in United Kingdom

Ab108411 is a monoclonal antibody that can be used in various laboratory applications. The core function of this product is to specifically detect and bind to its target antigen. However, detailed information about its intended use or application is not readily available.

Automatically generated - may contain errors

Lab products found in correlation

Anti rabbit hrp secondary, by Cell Signaling Technology (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) β actin hrp, by Abcam (1 mentions) Secondary anti mouse hrp, by Cell Signaling Technology (1 mentions) Abro1, by Abcam (1 mentions) Abraxas, by Abcam (1 mentions) G csf, by Santa Cruz Biotechnology (1 mentions) Nbp1 87156, by Novus Biologicals (1 mentions)

2 protocols using ab108411

Protein Quantification and Antibody Detection

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Protein concentrations were determined using the BCA Protein Assay Kit (23225, Thermo Fisher Scientific). The following antibodies were used: BRCC3: ab108411, Abcam, Cambridge, UK; Tubulin: T5168, Sigma-Aldrich; ABRO1: ab74333, Abcam; Abraxas: ab139191, Abcam; BABAM1: sc-160990, Santa Cruz, Dallas, TX, USA; UIMC1: 14466S, New England Biolabs, Ipswich, MA, USA; FLAG-tag (DYKDDDDK): 2368, Cell Signaling Technology, Danvers, MA, USA; HA-tag (YPYDVPDYA): 901533, BioLegend; G-CSF: sc-53292, Santa Cruz; β-Actin HRP: ab20272, Abcam; secondary anti-mouse HRP: 7076S, Cell Signaling; secondary anti-rabbit HRP: 7074S, Cell Signaling.

Meyer T., Jahn N., Lindner S., Röhner L., Dolnik A., Weber D., Scheffold A., Köpff S., Paschka P., Gaidzik V.I., Heckl D., Wiese S., Ebert B.L., Döhner H., Bullinger L., Döhner K, & Krönke J. (2019). Functional characterization of BRCC3 mutations in acute myeloid leukemia with t(8;21)(q22;q22.1). Leukemia, 34(2), 404-415.

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Western Blotting and Immunofluorescence Protocol

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The following antibodies were used for western blotting for human or mouse cells: Rabbit anti-human/mouse RAP80 (NBP1-87156; Novus Biologicals) at 1:1,000 for human cells, 1:100 for mouse cells; Rabbit anti-human RAP80 (A300-763A; Bethyl Laboratories) at 1:1,000. Rabbit anti-human MERIT40 (A302-515A; Bethyl Laboratories) at 1:1,000. Rabbit anti-human/mouse MERIT40 (D7Y5K; 12711S; Cell Signaling) at 1:1,000. Rabbit anti-human/mouse Abraxas1 (NBP1-22977; Novus Biologicals) at 1:1,000. Rabbit anti-mouse BRCC45 (EPR11858; ab177960; Abcam) at 1:1,000. Rabbit anti-human/mouse BRCC36 (ab108411; Abcam) at 1:50,000. Mouse anti-human P4D1 (sc-8017; Santa Cruz) at 1:1,000. Mouse anti-human GFP (sc-9996; Santa Cruz) at 1:500. Mouse anti-human/mouse Tubulin (3873S; Cell Signaling) at 1:10,000; Mouse anti-human Tubulin (12G10; DSHB) at 1:1,000. Mouse anti-human HA.11 (901502; BioLegend) at 1:1,000. The following antibodies were used for immunofluorescence experiment: Rabbit anti-mouse BRCA1 (homemade rabbit polyclonal antibody raised against the exon 11 region of mouse BRCA1) at 1:100; mouse anti-human BRCA1 (sc-6954; Santa Cruz) at 1:500; rabbit anti-mouse MERIT40 (polyclonal antibody raised against GST-MERIT40 as previously described [Shao et al., 2009 (link)]) at 1:200; mouse anti-mouse γH2AX (JBW301; Millipore) at 1:2,000; rabbit anti-human γH2AX (39117; Active Motif) at 1:500.

Jiang Q., Foglizzo M., Morozov Y.I., Yang X., Datta A., Tian L., Thada V., Li W., Zeqiraj E, & Greenberg R.A. (2022). Autologous K63 deubiquitylation within the BRCA1-A complex licenses DNA damage recognition. The Journal of Cell Biology, 221(9), e202111050.

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