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Beta galactosidase

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Beta galactosidase is an enzyme that catalyzes the hydrolysis of beta-galactosides into monosaccharides. It is commonly used in biochemical and molecular biology applications.

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Lab products found in correlation

Cdc42, by Abcam (1 mentions) Biotinylated goat anti rabbit secondary antibody, by Zymo Research (1 mentions) Occludin, by Zymo Research (1 mentions) Alexa fluor dye, by Thermo Fisher Scientific (1 mentions) Phospho histone h3, by Cell Signaling Technology (1 mentions) Axio imager, by Leica (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Antibodies to actin, by Abcam (1 mentions) Fibronectin, by Abcam (1 mentions) α sma, by Abcam (1 mentions) Timp 1, by Abcam (1 mentions) Mini trans blot cell and systems, by Bio-Rad (1 mentions) Phosphor smad2, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Ripa lysis buffer, by ATTO Corporation (1 mentions) Amelogenin, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Chicken anti-beta-galactosidase (9 citations) Chick anti-beta galactosidase (2 citations) Beta-galactosidase (LacZ) from chicken (2 citations) Chicken anti-beta galactosidase (ab9361, (2 citations) Chicken polyclonal primary antibody to beta-galactosidase (2 citations) Chicken anti-beta galactosidase (LacZ) (2 citations)

5 protocols using beta galactosidase

1

Histopathological Analysis of Lung Tissue

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Histopathological analysis was performed as described before25 (link). Briefly, mice were sacrificed and lung tissues were inflated and fixed in PFA, embedded in paraffin or frozen in optimal cutting temperature compound (OCT) and sectioned for hematoxylin and eosin (H&E) staining. Immunohistochemical and immunofluorescence analyses were performed as described34 (link). Antibodies against the following proteins were used: KI-67 (Novocastra Laboratories Ltd), CDC42 (Abcam), ZO1 (33-9100, Zymed); PAR6 (sc-14405, Santa Cruz), Occludin (353197a Zymed), Phalloidin (R415 Invitrogen), CCSP (Santa Cruz), SP-C (AB3786, Chemicon), Beta Galactosidase (ab9631, Abcam), Biotinylated goat anti-rabbit secondary antibody (ZYMED company), Alexa Fluor 555 or 488 conjugated anti-mouse, rat or rabbit IgG secondary antibodies (Invitrogen).
Zheng C., Wang Y., Yang L., Zhou S., Gao Y., Li F., Feng Y., Wang Z., Zhan L., Yan Q., Zhu X., Wong K.K., Chen Z, & Ji H. (2017). Cell Division Cycle 42 plays a Cell type-Specific role in Lung Tumorigenesis. Scientific Reports, 7, 10407.
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2

Detailed Immunofluorescence Staining Protocol

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For all experiments, dissection, fixation and staining protocols were performed as previously described (Cohen et al., 2018 ; Sawyer et al., 2017 (link)). Briefly, this involves dissection in PBS, paraformaldehyde fixation, and blocking and staining with normal goat serum along with Triton-X. Measurement of animal pyloric nuclear area represents the average of N≥30 cells per pylorus. The following antibodies were used in this study: Beta-Galactosidase (Abcam, ab9361, 1:1000), DCP1 (Cell Signaling, Asp261, 1:500), Phospho-Histone H3 (Cell Signaling, #9706, 1:1000). Secondary antibodies were Alexa Fluor dyes (Invitrogen, 1:500). Tissues were mounted in Vectashield (Vector Laboratories Inc.). Images were obtained with an upright Zeiss AxioImager with Apotome.2 processing, inverted Leica SP5 or Andor Dragonfly Spinning Disk Confocal. Image analysis was performed using ImageJ (Schneider et al., 2012 (link)), including adjusting brightness/contract, Z projections, cell counts, and integrated density quantification. Image stitching (Fig3, FigS3C–D) was performed using ImageJ grid/collection stitching plugin (Schneider et al., 2012 (link)).
Cohen E., Peterson N.G., Sawyer J.K, & Fox D.T. (2021). Accelerated cell cycles enable organ regeneration under developmental time constraints in the Drosophila hindgut. Developmental cell, 56(14), 2059-2072.e3.
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3

Immunohistochemistry of cancer samples

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The study was approved by the Institutional Review Board at Thomas Jefferson University. Pathological analyses were performed on skin, esophagus, non-small cell lung cancer (NSCLC) and diffuse large B cell lymphoma (DLBCL) samples. The samples had been fixed in 10% neutral buffered formalin and embedded in paraffin for hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining. The streptavidin–horse radish peroxidase method was used for IHC staining as previously described1 (link). Primary antibodies used were PTHrP(Santa Cruz, sc-20728), and beta galactosidase (Abcam, Cambridge, MA, ab96239). MCT1 antibody was kindly provided by Dr Nancy Philp and has been extensively characterized1 (link).
Mills T.A., Orloff M., Domingo-Vidal M., Cotzia P., Birbe R., Draganova-Tacheva R., Martinez-Cantarin M.P., Tuluc M, & Martinez-Outschoorn U. (2015). PTHrP-linked Hypercalcemia in a Melanoma Patient Treated with Ipilimumab: Hormone source and Clinical and Metabolic Correlates. Seminars in oncology, 42(6), 909-914.
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4

Quantifying Extracellular Matrix Remodeling Factors

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Cells (2×105 per well) were transfected with Ad-RLN for four hours, and lysed in RIPA lysis buffer (ATTO Corp., Tokyo, Japan) containing protease inhibitor (Pierce, Rockford, IL, USA). Meanwhile, the culture medium was analysed for RLN protein expression by Ad-RLN. Lysates and culture medium were resolved on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and proteins were then transferred to polyvinylidenedifluoride (Pierce) membranes using a transfer system (Mini Trans-Blot® Cell and systems, Bio-Rad, Hercules, CA, USA). The blots were incubated with specific antibodies against MMP-1, MMP-13 (Young In Frontier Co., Ltd., Seoul, Korea), ERK1/2, phosphor-ERK1/2 Smad2, phosphor-Smad2 (Cell Signaling Technology, Danvers, MA, USA), beta galactosidase, α-SMA, fibronectin, TIMP 1, and TIMP 4 (Abcam®, Cambridge, UK). After reacting with secondary antibodies, immunoreactive bands were visualized by a western blot detection system (ATTO Corp.). To verify the amounts of loaded proteins, blots were stripped of bound antibodies and re-probed using antibodies to actin (Abcam®).
Kang Y.M., Lee H.M., Moon S.H., Kang H, & Choi Y.R. (2017). Relaxin Modulates the Expression of MMPs and TIMPs in Fibroblasts of Patients with Carpal Tunnel Syndrome. Yonsei Medical Journal, 58(2), 415-422.
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5

Mouse Embryonic Tissue Analysis

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Mouse embryos or heads were dissected in phosphate-buffered saline (PBS). Embryos were fixed with 4% paraformaldehyde-PBS solution for 0.5–4 hours. Following fixation, samples were dehydrated through graded ethanol, embedded in paraffin wax and sectioned (7 μm). Standard Hematoxylin and Eosin was used to examine tissue morphology as previous described [157 (link)]. For immunofluorescence (IF) assays, slides were boiled in 10mM sodium citrate solution (pH 6.0) for 20 minutes for antigen retrieval. They were then incubated with 20% goat serum-PBST for 30 min at room temperature, and then with antibodies against Ki67 (Abcam, 1:200), amelogenin (Santa Cruz, 1:200), Lats1 (Cell signaling, 1:200) and pYap (Cell signaling, 1:200) and Beta-galactosidase (Abcam, ab9361, 1:50) at 4 oC overnight. The slides were treated with FITC (Alexa-488)- or Texas Red (Alexa-555)-conjugated Secondary antibody for 30 minutes at room temperature for detection (Invitrogen, 1:500). Nuclear counterstaining was performed using DAPI-containing mounting solution.
Sun Z., da Fontoura C.S., Moreno M., Holton N.E., Sweat M., Sweat Y., Lee M.K., Arbon J., Bidlack F.B., Thedens D.R., Nopoulos P., Cao H., Eliason S., Weinberg S.M., Martin J.F., Moreno-Uribe L, & Amendt B.A. (2018). FoxO6 regulates Hippo signaling and growth of the craniofacial complex. PLoS Genetics, 14(10), e1007675.
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