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Il 1β 3a6

Manufactured by Cell Signaling Technology
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IL-1β [3A6] is an antibody product manufactured by Cell Signaling Technology. It is designed to detect interleukin-1 beta (IL-1β), a pro-inflammatory cytokine involved in various cellular processes.

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Lab products found in correlation

Protease and phosphatase inhibitor, by Thermo Fisher Scientific (2 mentions) β actin antibody, by Cell Signaling Technology (2 mentions) C fos, by Cell Signaling Technology (2 mentions) 0.2 μm nitrocellulose membrane, by Bio-Rad (2 mentions) Nlrp3 d4d8t, by Cell Signaling Technology (2 mentions) Chemidoc mp imaging system, by Bio-Rad (2 mentions) Clarity western ecl substrate, by Bio-Rad (2 mentions) Caspase 1 p20 casper 1, by Adipogen (2 mentions) Secondary peroxidase labeled anti mouse or anti rabbit immunoglobulin g antibodies, by Jackson ImmunoResearch (1 mentions) Anti tubulin antibody t5168, by Merck Group (1 mentions) Mini protean tgx gel, by Bio-Rad (1 mentions) Ultraview universal dab detection kit, by Agilent Technologies (1 mentions) Benchmark xt, by Roche (1 mentions) Cd8 4b11, by Leica (1 mentions)

Close spelling variants of this product

Anti-IL-1β (3A6) Anti-IL-1β (clone 3A6, IL-1β

4 protocols using il 1β 3a6

1

Protein Expression and Immunoblotting

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Whole cell lysates were suspended in RIPA buffer containing protease and phosphatase inhibitors (Thermo Scientific). Lysates were separated in SDS-PAGE and transferred to a nitrocellulose membrane, 0.2 μm (Bio-Rad Laboratories). The membrane was incubated with the following primary antibodies: phospho-MPK1/MPK2 polyclonal [Ser296, Ser318] (Thermo Scientific) and c-Fos (Cell Signaling), IL-1β [3A6] (Cell Signaling), Caspase-1 p20 [Casper-1] (Adipogen Life Sciences) (Thermo Scientific), NLRP3 [D4D8T] (Cell Signaling). Normalization was performed by probing the membrane with β-Actin antibody (Cell Signaling). Chemiluminescence detection was performed with Clarity™ Western ECL Substrate (Bio-Rad Laboratories), using the ChemiDoc™ MP Imaging System (Bio-Rad).
Drummond R.A., Swamydas M., Oikonomou V., Zhai B., Dambuza I.M., Schaefer B.C., Bohrer A.C., Mayer-Barber K.D., Lira S.A., Iwakura Y., Filler S.G., Brown G.D., Hube B., Naglik J.R., Hohl T.M, & Lionakis M.S. (2019). CARD9+ Microglia Promote Antifungal Immunity via IL-1β and CXCL1-mediated Neutrophil Recruitment. Nature immunology, 20(5), 559-570.
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2

Western Blot Analysis of NLRP3 Inflammasome

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Cells were harvested, and culture supernatants were concentrated 30-fold using Amicon filter. Equal amounts of proteins were electrophoresed on Mini-PROTEAN® TGX™ gels (Bio-Rad Laboratories, Hercules, CA, USA) and transferred to nitrocellulose membranes as described [45 (link)]. Antibodies to NLRP3 (D2P5E), caspase-1 (D7F10) and IL-1β (3A6) were purchased from Cell Signaling Technology; antibodies to ASC (F-9) and c-Myc (9E10) were purchased from Santa Cruz Biotechnology; an anti-Tubulin (T5168) antibody was purchased from Sigma-Aldrich. Secondary peroxidase-labeled anti-mouse or anti-rabbit immunoglobulin G antibodies were purchased from Jackson ImmunoResearch Laboratories, West Grove, PA, USA.
Kim N.E., Kim D.K, & Song Y.J. (2021). SARS-CoV-2 Nonstructural Proteins 1 and 13 Suppress Caspase-1 and the NLRP3 Inflammasome Activation. Microorganisms, 9(3), 494.
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3

Immunohistochemical Analysis of Hepatic Cytolysis

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Three patients with persistent hepatic cytolysis underwent liver and digestive system biopsies.
In addition, one patient underwent skin biopsy twice for rashes. The biopsy samples were fixed in formalin and embedded in paraffin. Four-micrometer sections were generated and stained with hematoxylin-eosin-saffron. An immunohistochemical study was carried out using an automated immunohistochemistry system with antibodies against the following: CD20 (L26, Dako, 1/20e), CD3 (polyclonal rabbit, Dako, 1/100e), CD4 (4B12, Leica, 1/40e), CD8 (4B11, Leica, 1/50e), NLRP3 (HPA012878, Atlas antibodies, 1/400e), IL-1β (3A6, Cell Signaling, 1/100e), and NF-κB p65 (D14E12, Cell Signaling, 1/1500e). Briefly, 4-µm sections were prepared from paraffin blocks and placed on Superfrost plus slides. After antigen unmasking with target retrieval solution, citrate pH 6 or 9 was added according to the antibodies to be used, and the immunohistochemical study was carried out by an immunohistochemistry automaton (Ventana Benchmark XT). After incubation with the primary antibody (20 to 60 min), detection was carried out with an Ultraview Universal DAB Detection Kit (Dako) indirect detection kit according to the supplier's instructions.
A biopsy sample of chronic active colitis was used as an immunohistochemical control for some antibodies.
Deshayes S., Bazille C., El Khouri E., Kone-Paut I., Giurgea I., Georgin-Lavialle S., Martin Silva N., Dumont A., Ollivier I., Amselem S., de Boysson H, & Aouba A. (2021). Chronic hepatic involvement in the clinical spectrum of A20 haploinsufficiency. Liver international : official journal of the International Association for the Study of the Liver, 41(8).
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4

Protein Expression and Immunoblotting

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Whole cell lysates were suspended in RIPA buffer containing protease and phosphatase inhibitors (Thermo Scientific). Lysates were separated in SDS-PAGE and transferred to a nitrocellulose membrane, 0.2 μm (Bio-Rad Laboratories). The membrane was incubated with the following primary antibodies: phospho-MPK1/MPK2 polyclonal [Ser296, Ser318] (Thermo Scientific) and c-Fos (Cell Signaling), IL-1β [3A6] (Cell Signaling), Caspase-1 p20 [Casper-1] (Adipogen Life Sciences) (Thermo Scientific), NLRP3 [D4D8T] (Cell Signaling). Normalization was performed by probing the membrane with β-Actin antibody (Cell Signaling). Chemiluminescence detection was performed with Clarity™ Western ECL Substrate (Bio-Rad Laboratories), using the ChemiDoc™ MP Imaging System (Bio-Rad).
Drummond R.A., Swamydas M., Oikonomou V., Zhai B., Dambuza I.M., Schaefer B.C., Bohrer A.C., Mayer-Barber K.D., Lira S.A., Iwakura Y., Filler S.G., Brown G.D., Hube B., Naglik J.R., Hohl T.M, & Lionakis M.S. (2019). CARD9+ Microglia Promote Antifungal Immunity via IL-1β and CXCL1-mediated Neutrophil Recruitment. Nature immunology, 20(5), 559-570.
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