Nicolet nexus 870 ft ir e s p spectrometer

Melting points were measured using a XT-4 Boetius micro melting point apparatus (Beijing, China). Specific rotations were obtained on a Perkin-Elmer 341 digital polarimeter (Waltham, MA, USA). IR spectra were taken as the KBr discs on a Thermo Nicolet Nexus 870 FT-IR E.S.P. spectrometer (Beijing, China). High resolution electrospray ionization mass spectroscopy (HR-ESI-MS) spectra were recorded on an Agilent 1260 UPLC DAD 6530 Q-TOF mass spectrometer (Santa Clara, CA, USA). Nuclear magnetic resonance (NMR) data were acquired in pyridine-d₅ on a Bruker DRX500 NMR spectrometer (Beijing, China) with ¹H- and ¹³C-NMR observed at 500 and 125 MHz. Silica gel (200–300 mesh; Qingdao Marine Chemical Factory, China), RP-C18 (40–60 μm, Merck, Darmstadt, Germany) and Sephadex LH-20 (GE Healthcare Life Sciences, Shanghai, China) were used for column chromatography (CC). Thin-layer Chromatography (TLC) analysis was performed using on Silica gel GF254 plates (10–40 μm; Qingdao Marine Chemical Factory, Qingdao, China).

In nanotechnology, the FTIR method is used to confirm whether a specific species is functionalized or not functionalized with another compound and/or to confirm the accurate coating of a surface with specific compounds.
Infrared waves are of low energy levels and cannot break bonds or cause electron transfers. Each frequency represents the presence of a specific functional group in the sample. Therefore, useful information can be obtained regarding the molecular structure of organic compounds.
In this study, spectral analysis was performed for chitosan (CS), stearic acid, stearate–chitosan nanogel (SCS nanogel), and apigenin and apigenin-loaded chitosan–stearate nanogel (APG–SCS) using FTIR by employing a Thermo Nicolet Nexus 870 FT-IR ESP spectrometer (manufactured in the USA).