X-ray diffraction measurements (XRD) were performed on a D8 Advance diffractometer with Cu Kα radiation λ = 0.154 nm (Bruker, Karlsruhe, Germany). The transmission electron microscopy (TEM) images were obtained using a JEM-2100F transmission electron microscope (Hitachi, Tokyo, Japan). Scanning electron micrographs (SEM) were obtained from an S-4800 scanning electron microscope (Hitachi). Absorption spectra were acquired on a Lambda 650 UV-vis spectrophotometer (PerkinElmer, New York, US) with the wavelength ranging from 200.00 to 1000.00 nm. The impedance of humidity sensors were measured by Zennium workstation (Zahner, Kronach, Germany).
Lambda 650 uv vis spectrophotometer
Manufactured by PerkinElmer
Sourced in United States
The Lambda 650 UV-vis spectrophotometer is a laboratory instrument designed for the measurement of the absorption or transmission of light in the ultraviolet and visible regions of the electromagnetic spectrum. It provides accurate and reliable data for a wide range of applications, including chemical analysis, material science, and life science research.
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Lab products found in correlation
Jem 2100f transmission electron microscope, by Hitachi (3 mentions)
S 4800 scanning electron microscope, by Hitachi (2 mentions)
Zennium workstation, by Zahner (2 mentions)
F 4600 spectrophotometer, by Hitachi (2 mentions)
D8 advance diffractometer, by Bruker (1 mentions)
Quanta 200f, by Thermo Fisher Scientific (1 mentions)
D8 advance, by Bruker (1 mentions)
Fls1000 fluorometer, by Edinburgh Instruments (1 mentions)
Avance 400 nmr spectrometer, by Bruker (1 mentions)
Deuterated solvents for nmr, by Merck Group (1 mentions)
Oxford diffractometer, by Rigaku (1 mentions)
Tcs sp5 confocal microscope, by Leica (1 mentions)
Microtof q 2 mass spectrometer, by Bruker (1 mentions)
Alpha t ft ir spectrometer, by Bruker (1 mentions)
Fts 6000 ftir spectrometer, by Bio-Rad (1 mentions)
Etc 273t, by Jasco (1 mentions)
Fp 6500 spectrofluorometer, by Jasco (1 mentions)
R5509 72 inp ingaas, by Hamamatsu Photonics (1 mentions)
17 protocols using lambda 650 uv vis spectrophotometer
1
Comprehensive Materials Characterization
2
Spectroscopic Analysis of Wood Samples
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A Lambda 650 UV/Vis-spectrophotometer of PerkinElmer was used for recording UV/Vis-absorption spectra. The spectra were recorded in a wavelength range of 416 nm to 404 nm in steps of 1 nm with a rate of 4.45 nm s−1. Scanning electron microscopy was carried out on thin-cuts of the wood samples that were sputter coated with platinum/palladium (60/40%) prior to the measurement. Images were acquired with a Quanta 200F from FEI Company at an acceleration voltage of 10 kV. Particle sizes were evaluated by line measurements with the software ImageJ (NIH, USA).
3
Structural and Physicochemical Characterization
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The structural characteristics of samples were analyzed through the X-ray diffraction (XRD) (Bruker, Karlsruhe, Germany) patterns (2θ = 15–90°, rate of 6 deg min−1) from a Bruker D8 Advance X-ray Diffraction using Cu Kα radiation. A field emission scanning electron microscope (SEM) (Hitachi, Japan) was used to characterize the morphology of the samples. The chemical bonding state of samples was analyzed by X-ray photoelectron spectroscopy (XPS) (Bruker, Kronach, Germany) using a Thermo VG ESCA Lab250 spectrometer. Absorption spectra with a wavelength of 200.00 to 1000.00 nm were obtained by a Lambda 650 UV-Vis spectrophotometer (Perkin Elmer, Karlsruhe, Germany) with a gold coating. The impedance of humidity sensors was measured by a Zennium workstation (Zahner, Kronach, Germany).
4
Spectrophotometric Analysis of DOX and 4-pep-DOX Binding to dsDNA
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The concentrations of the studied compounds (DOX and 4-pep–DOX) were confirmed spectrophotometrically based on the absorbance and the value of the molar extinction coefficient determined at 480 nm (ε480 = 11500 M−1 cm−1) [44 (link)]. UV-Vis spectrophotometric experiments were carried out in 10 mM Tris-HCl buffer (pH 7.2) at 25 °C using a PerkinElmer Lambda 650 UV/Vis spectrophotometer (Walthnam, MA, USA). UV-Vis absorption spectra of DOX and 4-pep–DOX (18.0 µM) were recorded in the absence and presence of increasing concentrations of dsDNA, up to 1.90 µM. In the performed spectrophotometric experiments, 2 mL of DOX and 4-pep–DOX at 18.0 µM were titrated with ten 10 μL aliquots of dsDNA solution at 40 µM.
5
Quantum Yield Measurement of Enantiomers
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Overall quantum
yields were measured by adopting the relative method. Fluorescein
in 0.1 M NaOH (fluorescence quantum yield of 0.9) was used as the
standard. Absorption spectra were recorded with a PerkinElmer Lambda
650 UV–vis spectrophotometer. Emission spectra were recorded
with an Edinburgh FLS1000 fluorometer and corrected for excitation
intensity and detector sensitivity. The samples were dissolved in
methanol, while keeping their absorbance lower than 0.1. We obtained
the same values of overall quantum yield for the two enantiomers.
yields were measured by adopting the relative method. Fluorescein
in 0.1 M NaOH (fluorescence quantum yield of 0.9) was used as the
standard. Absorption spectra were recorded with a PerkinElmer Lambda
650 UV–vis spectrophotometer. Emission spectra were recorded
with an Edinburgh FLS1000 fluorometer and corrected for excitation
intensity and detector sensitivity. The samples were dissolved in
methanol, while keeping their absorbance lower than 0.1. We obtained
the same values of overall quantum yield for the two enantiomers.
6
Cytochrome c Reduction by POR Enzyme
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The dual labeled POR enzyme (0.045 µM) in buffer containing Tris 50 mM (pH 7.3) and 100 mM NaCl was incubated with 50 µM cytochrome c in a 1 mL cuvette for 2–3 min. After recording a baseline for 1 min, the reaction was initiated by addition of 1 mM NADPH and the amount of cytochrome c reduced was monitored by the time-dependent increase in absorbance at 550 nm on a Perkin-Elmer Lambda 650 UV/Vis spectrophotometer (εCyt-c = 21.2 mM−1 cm−1). The slope of the curve in the linear region was used to determine the catalytic activity39 (link).
7
Kinetic Assay for Human DHFR Activity
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A hsDHFR assay kit (CS0340, Sigma-Aldrich) was used following the manufacturer’s protocol. This assay is based on DHFR’s ability to reduce dihydrofolate (DHF) to tetrahydrofolate (THF) in the presence of NADPH, resulting in a reduction of absorbance at 340 nm. Briefly, a stock solution of hsDHFR (1.5 × 10−3 U), DHF (10 mM), and NADPH (10 mM) were dissolved in assay buffer. Methotrexate (2.9 mM), TMP (2.9 mM), and 14a (2.9 mM) were dissolved in DMSO and then further diluted to 10 μM in assay buffer. To prepare the reaction mix, hsDHFR (12.5 μL) was added to 972.5 μL assay buffer. Next, 5 μL potential inhibitor (MTX [50 nM], TMP [50 nM], or 14a [50 nM]) and 6 μL NADPH were added, followed by the addition of 6 μL DHF. Using a PerkinElmer LAMBDA 650 UV/Vis spectrophotometer, reaction progression was recorded with 1-s resolution for 150 s. Initial velocities of the reactions were determined using the molar absorbance difference of 12.3 mM−1cm−1.
8
Fluorescence and Absorption Spectroscopy
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Fluorescence measurements were carried out on an F-4600 spectrophotometer (Hitachi, Tokyo, Japan) with the wavelength ranging from 200.00 nm to 900.00 nm. The spectra was obtained under the condition that photomultiplier tube (PMT) voltage was 700 V, excitation and emission slits was 5 nm and excitation wavelength was 350 nm. The high solution transmission electron microscopic (HR-TEM) images were obtained from a JEM-2100F transmission electron microscope (Hitachi, Tokyo, Japan). Absorption spectra were acquired on a Lambda 650 UV-vis spectrophotometer (PerkinElmer, Waltham, MA, USA) with the wavelength ranging from 200.00 to 1000.00 nm.
9
Characterization of Organic Compounds by Spectroscopic Methods
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All solvents (analytical grade and spectroscopic grade) were obtained from Finar (India) and Spectrochem (India) and the solvents were purified using standard literature methods. Deuterated solvents for NMR were bought from Sigma-Aldrich (India) and used as received. All metal salts and other chemicals were obtained from Alfa-Aesar (India), Spectrochem (India) and Merck (India). Melting points were measured using a BUCHI M-500 instrument. Fourier transform infrared (FT-IR) spectra were recorded on a BRUKER ALPHA-T FT-IR spectrometer using KBr pellets. A PerkinElmer model Lambda 650 UV-vis spectrophotometer was used for recording the absorption spectra. Emission spectra were recorded on a Fluoromax 4P Spectro-fluorometer (Horiba-Jobin-Mayer, Edison, NJ, USA). The bioimaging experiments were carried out using a Leica TCS SP5 confocal microscope and the fluorescence lifetime was measured using a time-correlated single-photon counting (TCSPC) spectrometer (Edinburgh, OB920) instrument. 1H and 13C NMR spectra were recorded on a Bruker AVANCE 400 NMR spectrometer. A Bruker microTOF-Q II mass spectrometer was used for electrospray ionization mass spectrometry (ESI-MS) analysis of the compounds. X-ray data were collected using a Rigaku Oxford diffractometer with graphite-monochromated Cu-Kα (λ = 1.54178 Å) radiation at 110(2) K.
10
Spectroscopic Analysis of Hydrogel Samples
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Fourier transform infrared (FT-IR) spectroscopy measurements were carried out at room temperature on a Bio-Rad FTS 6000 FT-IR spectrometer (Bio-Rad, Hercules, CA, USA) equipped with an attenuated total reflection (ATR) accessory (Ge crystal, 45° angle of incidence) and a mercury-cadmium-telluride detector. All spectra were recorded in the wavenumber range of 750–4000 cm−1. The hydrogel samples were lyophilized and placed on the ATR crystal. Spectra were averaged over 128 scans at a resolution of 4 cm−1 with baseline correction and smoothing, using WIN-IR PRO version 2.6 software.
UV spectra were acquired at room temperature on a Perkin Elmer Lambda 650 UV/Vis Spectrophotometer (Perkin Elmer, Waltham, MA, USA), using a 1 cm cell path length for data between 300 and 600 nm, with a 1 nm sampling interval.
UV spectra were acquired at room temperature on a Perkin Elmer Lambda 650 UV/Vis Spectrophotometer (Perkin Elmer, Waltham, MA, USA), using a 1 cm cell path length for data between 300 and 600 nm, with a 1 nm sampling interval.
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