Macrophages were cultured on chamber slides. They were untreated or treated with cocaine (10 μM), infected or uninfected with HIV-1 (10 ng/ml) for 72 h. They were fixed in 4% paraformaldehyde and blocked with 5% normal goat serum in PBS/Triton X100 (1 h). Cells were then incubated with primary antibodies overnight at 4 °C, washed thrice with PBS, and stained with AlexaFluor 546–labeled anti–mouse IgG antibody or AlexaFluor 488–labeled anti–rabbit IgG antibody (Molecular Probes®; Invitrogen) for 2 hours. Subsequently, cells were washed thrice with PBS, and slides were mounted using Prolong Gold antifade with DAPI (4′,6-diamidino-2-phenylindole; Invitrogen). Slides were examined under a Zeiss 880 Meta confocal microscope (Carl Zeiss Microimaging, LLC, Thornwood, NY), and images were acquired using ZEN2 software (Carl Zeiss).
880 meta confocal microscope
Manufactured by Zeiss
The Zeiss 880 Meta confocal microscope is a high-performance imaging system designed for advanced research applications. It features a fully motorized and automated confocal design, providing high-resolution, 3D imaging capabilities. The microscope is equipped with a range of laser sources and detectors, enabling the study of various fluorescent samples across multiple channels. The Zeiss 880 Meta offers users a powerful and versatile platform for their research needs.
Automatically generated - may contain errors
Lab products found in correlation
Zen 2, by Zeiss (4 mentions)
Prolong gold antifade with 4 6 diamidino 2 phenylindole dapi, by Thermo Fisher Scientific (2 mentions)
Alexafluor 488 labeled anti rabbit igg antibody, by Thermo Fisher Scientific (1 mentions)
Prolong gold antifade with dapi, by Thermo Fisher Scientific (1 mentions)
Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions)
4 protocols using 880 meta confocal microscope
1
Cocaine and HIV-1 Impacts on Macrophages
2
Visualizing HIV-1 Infection in DCs and T-cells
3
Methamphetamine Effects on CD4+ T-cell Imaging
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CD4+ T-cells were cultured on chamber slides. They were starved for 2 hours, and then untreated or treated with Meth (100 μM) for 24 hours. They were fixed in 4% paraformaldehyde and blocked with 5% normal goat serum in PBS/0.1% Triton X-100 (1 hour). Cells were then incubated with primary antibodies overnight at 4 °C, washed thrice with PBS, and stained with AlexaFluor 488–labeled anti–rabbit IgG antibody and AlexaFluor 594–labeled anti–mouse IgG antibody (Molecular Probes®; Invitrogen) for 2 hours. Subsequently, cells were washed thrice with PBS, and slides were mounted using Prolong Gold antifade with DAPI (4′,6-diamidino-2-phenylindole; Invitrogen). Slides were examined under a Zeiss 880 Meta confocal microscope (Carl Zeiss Microimaging, LLC, Thornwood, NY), and images were acquired using ZEN2 software (Carl Zeiss). Figures were made using Adobe Photoshop CS4 software (Adobe Systems, San Jose, CA).
4
Visualizing Exosome Uptake in T Cells
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Exosome pellets from uninfected and HIV-1 infected DCs resuspended in PBS (100 ng total protein content) were labeled with 5 μM CFSE for 30 minutes at 37 °C, followed by washing with 1X PBS to remove unbound dye at 100,000 × g. The labeled exosomes were incubated with T cells for 1 hour. Then T cells were fixed in 4% paraformaldehyde and blocked with 5% normal goat serum in PBS/Triton × 100 for 1 hour. Cells were then incubated with Rhodamine-phalloidin (Molecular Probes) for 2 hours. Subsequently, cells were washed thrice with PBS, and slides were mounted using Prolong Gold antifade with DAPI (4′,6-diamidino-2-phenylindole; Invitrogen). Slides were examined under a Zeiss 880 Meta confocal microscope (Carl Zeiss Microimaging, LLC, Thornwood, NY), and images were acquired using ZEN2 software (Carl Zeiss). Figures were made using Adobe Photoshop CS4 software (Adobe Systems, San Jose, CA).
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