Transwell chambers with 8 μm pore size membranes (BD Biosciences, Franklin Lakes, NJ, USA) were coated with collagen and 4×104 cells per well were seeded into the upper chamber. After 72 h, cells that had migrated to the bottom of the lower chamber were stained with crystal violet and evaluated colorimetrically.
Transwell chambers with 8 μm pore size membranes
Manufactured by BD
Sourced in United States
Transwell chambers with 8 μm pore size membranes are a lab equipment product designed for cell culture applications. The chambers feature a semi-permeable membrane with 8 μm sized pores, allowing for the study of cell migration and other cell-based assays.
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Lab products found in correlation
2 protocols using transwell chambers with 8 μm pore size membranes
1
Transwell-based Cell Migration Assay
2
MCF7 Cell Migration Assay
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was performed using transwell chambers with 8-μm pore-size membranes (BD Biosciences) according to manufacturer instructions with 5×104 MCF7 cells from 2 to 20% of FCS. The migration activity was quantified by blind counting of the migrated cells from 20 fields per chamber in 3 independent experiments (Mean ± SEM).
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