910 continuous flow cryostat

Manufactured by Oxford Instruments

The 910 continuous flow cryostat is a lab equipment product from Oxford Instruments. It is designed to provide a stable, controlled cryogenic environment for various applications. The cryostat utilizes a continuous flow of cryogenic liquid to maintain the desired temperature.

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Lab products found in correlation

Esr 300 spectrometer, by Oxford Instruments (6 mentions)

Spelling variants of this product

Continuous flow cryostat (14 citations) Cryostat (11 citations) ESR 910 continuous helium flow cryostat (2 citations)

8 protocols using 910 continuous flow cryostat

Quantification of Reduced mitoNEET Clusters

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The X-band EPR (Electron Paramagnetic Resonance) spectra were recorded using a Bruker model ESR-300 spectrometer equipped with an Oxford Instruments 910 continuous flow cryostat. Routine EPR conditions were: microwave frequency, 9.47 GHz; microwave power, 10.0 mW; modulation frequency, 100 kHz; modulation amplitude, 1.2 mT; temperature, 30 K; receive gain, 2×10⁵. The amount of the reduced mitoNEET [2Fe-2S] clusters was quantified as in [23 (link)].

Landry A.P., Cheng Z, & Ding H. (2015). Reduction of mitochondrial protein mitoNEET [2Fe-2S] clusters by human glutathione reductase. Free radical biology & medicine, 81, 119-127.

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Characterization of Protein Structure

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The circular dichroism (CD) spectra were recorded on a Jasco J-815 CD spectrometer (AgCenter Biotechnology Laboratories, LSU) at room temperature. The composition of secondary structures was obtained using the CDNN program [30 (link)]. The electron paramagnetic resonance (EPR) spectra were recorded at X-band on a Bruker ESR-300 spectrometer equipped with an Oxford Instruments 910 continuous flow cryostat. EPR conditions were as follows: microwave frequency, 9.45 GHz; microwave power, 10 mW; modulation frequency, 100 kHz; modulation amplitude, 2 mT; sample temperature, 10 K; receive gain, 1 × 10⁵.

Landry A.P, & Ding H. (2014). The N-Terminal Domain of Human DNA Helicase Rtel1 Contains a Redox Active Iron-Sulfur Cluster. BioMed Research International, 2014, 285791.

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EPR Spectroscopy of Paramagnetic Samples

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Electron paramagnetic resonance (EPR) spectra were recorded using a Bruker model X-band ESR-300 spectrometer equipped with an Oxford Instruments 910 continuous flow cryostat. EPR conditions: microwave frequency, 9.47 GHz; microwave power, 1.0 mW; modulation frequency, 100 kHz; modulation amplitude, 1.2 mT; temperature, 20 K; receiver gain, 2 × 10⁵.

Hoshino T., Noda N., Tsuneda S., Hirata A, & Inamori Y. (2001). Direct Detection by In Situ PCR of the amoA Gene in Biofilm Resulting from a Nitrogen Removal Process. Applied and Environmental Microbiology, 67(11), 5261-5266.

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Cryogenic EPR Spectroscopy Protocol

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The X-band Electron Paramagnetic Resonance (EPR) spectra were recorded using a Bruker model ESR-300 spectrometer equipped with an Oxford Instruments 910 continuous flow cryostat. Routine EPR conditions: microwave frequency, 9.47 GHz; microwave power, 10.0 mW; modulation frequency, 100 kHz; modulation amplitude, 1.2 mT; temperature, 20 K; receiver gain, 2×10⁵.

Wang Y., Lee J, & Ding H. (2019). Light-induced release of nitric oxide from the nitric oxide-bound CDGSH-type [2Fe-2S] clusters in mitochondrial protein Miner2. Nitric oxide : biology and chemistry, 89, 96-103.

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EPR Spectroscopy of X-band Samples

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The X-band EPR spectra were recorded using a Bruker model ESR-300 spectrometer equipped with an Oxford Instruments 910 continuous flow cryostat. Routine EPR conditions: microwave frequency, 9.47 GHz; microwave power, 10.0 mW; modulation frequency, 100 kHz; modulation amplitude, 1.2 mT; temperature, 20 K; receiver gain, 2 × 10⁵.

Fontenot C.R., Cheng Z, & Ding H. (2022). Nitric oxide reversibly binds the reduced [2Fe-2S] cluster in mitochondrial outer membrane protein mitoNEET and inhibits its electron transfer activity. Frontiers in Molecular Biosciences, 9, 995421.

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X-band EPR Spectroscopy of Samples

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The X-band Electron Paramagnetic Resonance (EPR) spectra were recorded using a Bruker model ESR-300 spectrometer equipped with an Oxford Instruments 910 continuous flow cryostat. Routine EPR conditions were: microwave frequency, 9.47 GHz; microwave power, 10.0 mW; modulation frequency, 100 kHz; modulation amplitude, 1.2 mT; temperature, 30 K; receiver gain, 2×10⁵.

Landry A.P., Wang Y., Cheng Z., Crochet R.B., Lee Y.H, & Ding H. (2016). Flavin nucleotides act as electron shuttles mediating reduction of the [2Fe-2S] clusters in mitochondrial outer membrane protein mitoNEET. Free radical biology & medicine, 102, 240-247.

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EPR Spectroscopy of Frozen Samples

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The X-band Electron Paramagnetic Resonance (EPR) spectra were recorded using a Bruker model ESR-300 spectrometer equipped with an Oxford Instruments 910 continuous flow cryostat. Routine EPR conditions were: microwave frequency, 9.47 GHz; microwave power, 1.0 mW; modulation frequency, 100 kHz; modulation amplitude, 1.2 mT; temperature, 20 K; receiver gain, 2×10⁵.

Tasnim H., Landry A.P., Fontenot C.R, & Ding H. (2020). Exploring the FMN binding site in the mitochondrial outer membrane protein mitoNEET. Free radical biology & medicine, 156, 11-19.

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EPR Spectroscopy of Cryogenic Samples

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The electron paramagnetic resonance (EPR) spectra were recorded at X-band on a Bruker ESR-300 spectrometer equipped with an Oxford Instruments 910 continuous flow cryostat. EPR conditions were: microwave frequency, 9.45 GHz; microwave power, 10 mW; modulation frequency, 100 kHz; modulation amplitude, 2 mT; sample temperature, 10 K; receive gain, 1 × 10⁵.

Cheng Z., Tan G., Wang W., Su X., Landry A.P., Lu J, & Ding H. (2014). Iron and Zinc Binding Activity of Escherichia coli Topoisomerase I Homolog YrdD. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 27(2), 229-236.

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