Ratlaps ctx 1 eia kit

Manufactured by Immunodiagnostic Systems

Sourced in United Kingdom, United States

The RatLaps CTX-I EIA kit is a laboratory diagnostic tool used for the measurement of C-terminal telopeptide of type I collagen (CTX-I) in rat serum or plasma samples. The kit employs an enzyme-linked immunosorbent assay (EIA) technique to quantify the levels of CTX-I, which is a biomarker of bone resorption.

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Lab products found in correlation

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Spelling variants of this product

RatLaps EIA kit (28 citations) RatLaps EIA (23 citations) CTX-1 (18 citations) RatLaps (18 citations) EIA kit (18 citations) RatLaps (CTX-I) EIA (15 citations) RatLaps CTX-I (5 citations) RatLap EIA Kit (2 citations)

15 protocols using ratlaps ctx 1 eia kit

Serum Biomarkers for Bone Turnover

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Serum samples were collected at the time of sacrifice and stored at −80°C. Serum levels of Pro-collagen type 1 N terminal propeptide (P1NP) were measured using the Rat/Mouse P1NP EIA kit (Immunodiagnostic Systems, Fountain Hills, AZ, USA) and serum C-terminal telopeptide of type I collagen (CTX-1) levels were measured using RatLaps (CTX-1) EIA kit (Immunodiagnostic Systems Inc., Gaithersburg, MD, USA). The MILLIPLEX MAP mouse angiogenesis magnetic bead panel – multiplex assay was used to measure serum VEGF-A levels (MAGPMAG-24K, EMD Millipore, Billerica, MA, USA). All the assays were performed as recommended by the manufacturers and all the samples were assayed in duplicate. A standard curve was generated from each kit, and the absolute concentrations were extrapolated from the standard curves. The coefficients of variations for inter-assay and intra-assay measurements were < 10% for all assays and are similar to the manufacturers’ references [28 (link),29 (link)].

Mohan G., Lay E.Y., Berka H., Ringwood L., Kot A., Chen H., Yao W, & Lane N.E. (2016). A novel hybrid compound LLP2A-Ale both prevented and rescued the osteoporotic phenotype in a mouse model of glucocorticoid-induced osteoporosis. Calcified tissue international, 100(1), 67-79.

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Measuring Serum CTX-I Levels

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Serum samples were collected at the time of euthanization and stored at −80 °C. Serum C-terminal telopeptide of type I collagen (CTX-I) levels, a biomarker of bone resorption, were measured using RatLaps (CTX-1) EIA kit (Immunodiagnostic Systems Inc., Gaithersburg, MD, USA). the assays were performed follow the manufacturer’s protocol and all the samples were accessed in duplicate. The absolute concentrations were extrapolated from the standard curves which generated from the kit.

Ji B., Zhang Z., Guo W., Ma H., Xu B., Mu W., Amat A, & Cao L. (2018). Isoliquiritigenin blunts osteoarthritis by inhibition of bone resorption and angiogenesis in subchondral bone. Scientific Reports, 8, 1721.

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Serum Biomarkers of Bone and Inflammation

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P1NP levels in mouse serum were determined using the Rat/Mouse P1NP EIA kit (Immunodiagnostic Systems). C-terminal telopeptide of type I collagen (CTX-I) levels in fasting mouse serum were determined using the RatLaps CTX-I EIA kit (Immunodiagnostic Systems). Serum cytokine arrays were performed using 44-Plex technology (MD44) from Eve Technologies. IL-20, IL-11, Lif, and IL-1β levels were determined using mouse IL-20 (EMIL20; ThermoFisher), IL-11 (EMIL11; ThermoFisher), Lif (MLF00; ThermoFisher), and IL-1β ELISA kits (5018265; ThermoFisher). Absorbance was measured at 450 nm using Clariostar Plate Reader or Molecular Devices FilterMax.

Kim P.G., Niroula A., Shkolnik V., McConkey M., Lin A.E., Słabicki M., Kemp J.P., Bick A., Gibson C.J., Griffin G., Sekar A., Brooks D.J., Wong W.J., Cohen D.N., Uddin M.M., Shin W.J., Pirruccello J., Tsai J.M., Agrawal M., Kiel D.P., Bouxsein M.L., Richards J.B., Evans D.M., Wein M.N., Charles J.F., Jaiswal S., Natarajan P, & Ebert B.L. (2021). Dnmt3a-mutated clonal hematopoiesis promotes osteoporosis. The Journal of Experimental Medicine, 218(12), e20211872.

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Serum CTX-I Quantification in Mice

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The serum level of C-terminal telopeptide of type I collagen (CTX-I) from fasted mice was measured using a RatLaps (CTX-I) EIA Kit from Immunodiagnostic Systems (Boldon, UK) according to the manufacturer’s instructions.

Park P.S., Mun S.H., Zeng S.L., Kim H., Bae S, & Park-Min K.H. (2020). NRF2 Is an Upstream Regulator of MYC-Mediated Osteoclastogenesis and Pathological Bone Erosion. Cells, 9(9), 2133.

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Quantitative Determination of Bone Markers

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Quantitative determination of PINP and CTX-I was performed using a Rat/Mouse PINP EIA Kit (AC-33F, Immunodiagnostic systems, Tyne and Wear, UK) and a RatLaps CTX-I EIA Kit (AC-06F1, Immunodiagnostic systems), respectively, according to the manufacturer’s instructions.

Yahiro Y., Maeda S., Morikawa M., Koinuma D., Jokoji G., Ijuin T., Komiya S., Kageyama R., Miyazono K, & Taniguchi N. (2020). BMP-induced Atoh8 attenuates osteoclastogenesis by suppressing Runx2 transcriptional activity and reducing the Rankl/Opg expression ratio in osteoblasts. Bone Research, 8, 32.

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Murine Serum Biomarker Quantification

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Mice were fasted for 6 hours before blood collection. Mouse TNF-α Duo-Set ELISA kit (R&D), mouse TRAP5b assay kit (Immunodiagnostic Systems), mouse ICTP ELISA kit (Biotang Inc.), and RatLaps^™ CTX-I EIA kit (Immunodiagnostic Systems) were used. Serum was separated from blood with Vacutainer collection tube (BD) and stored at −80 °C until use.

Kittaka M., Mayahara K., Mukai T., Yoshimoto T., Yoshitaka T., Gorski J.P, & Ueki Y. (2017). Cherubism mice also deficient in c-Fos exhibit inflammatory bone destruction executed by macrophages that express MMP14 despite the absence of TRAP+ osteoclasts. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(1), 167-181.

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Serum Analysis of Bone and Immune Markers

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ELISA was performed on serum samples. CTX was measured using the RatLaps CTX-I EIA kit (Immunodiagnostic Systems, Tyne & Wear, UK), OIM serum was diluted up to 1:8 to ensure values fell within the standard curve. Cytokines were measured with Mouse TNFα Quantikine High Sensitivity ELISA kit and Mouse IL1α Quantikine ELISA kit (R&D Systems). All assays were performed according to the manufacturer’s instructions.

Matthews B.G., Roeder E., Wang X., Aguila H.L., Lee S.K., Grcevic D, & Kalajzic I. (2017). Splenomegaly, myeloid lineage expansion and increased osteoclastogenesis in osteogenesis imperfecta murine. Bone, 103, 1-11.

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Calvarial Bone Resorption Assay

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Calvariae from 5-d-old wild-type or DKO mice were isolated aseptically, cleaned, and cultured for 16 h at 37°C in 0.5 ml of BGJb medium (Life Technologies) containing 1 mg/ml BSA (fraction V; Sigma-Aldrich; Moxon et al., 2015 (link)). Half calvariae were transferred to fresh medium with 0.1 μM parathyroid hormone (H-4835.0005; Bachem) in the presence or absence of an anti–galectin-3 monoclonal antibody (sc-32790L; Santa Cruz) or an anti-Lrp1 monoclonal antibody (MA1-27198; Thermo Fisher Scientific), and cultured for an additional 5 d. Culture supernatant was collected for bone resorption marker CTX-I level detection using RatLaps CTX-I EIA kit (AC-06F1; Immunodiagnostic Systems). The half calvariae were either fixed for immunostaining with an anti-TRAP polyclonal antibody (sc-30833; Santa Cruz), an anti–galectin-3 monoclonal antibody (#125401; Biolegend; clone M3/38), or an anti-V-type proton pump-3 (Vpp3) polyclonal antibody (ab200839; Abcam) as describe above or snap-frozen for TRAP activity assay.

Zhu L., Tang Y., Li X.Y., Kerk S.A., Lyssiotis C.A., Sun X., Wang Z., Cho J.S., Ma J, & Weiss S.J. (2023). Proteolytic regulation of a galectin-3/Lrp1 axis controls osteoclast-mediated bone resorption. The Journal of Cell Biology, 222(4), e202206121.

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Serum Biomarker Quantification in Mice

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Mouse TNF-α Duo-Set ELISA kit (R&D Systems, Minneapolis, MN, USA), mouse TRAP5b assay kit (Immunodiagnostic Systems, Boldon, UK), and RatLaps CTX-I EIA kit (Immunodiagnostic Systems) were used to measure serum levels of TNF-α, TRAP5b, and CTX. Serum was separated from blood with Vacutainer collection tube (BD, Franklin Lakes, NJ, USA) and stored at −80 °C until use.

Kittaka M., Yoshimoto T., Hoffman H., Levitan M.E, & Ueki Y. (2020). RANKL-independent osteoclastogenesis in the SH3BP2 cherubism mice. Bone Reports, 12, 100258.

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Serum Osteocalcin and CTX Measurement

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Circulating osteocalcin and CTX in serum was measured using a mouse Osteocalcin enzyme immunoassay kit (Thermo Fisher) and RatLaps (CTX-I) EIA kit (Immunodiagnostic Systems, Boldon, UK) respectively according to the manual provided by manufacturers. Blood was collected by retro-orbital bleeding into 1.7 mL microcentrifuge tubes. Blood was then kept at room temperature for one hour and centrifuged at 1500 x g for 10 min to separate serum from cells.

Li X., Han L., Nookaew I., Mannen E., Silva M.J., Almeida M, & Xiong J. (2019). Stimulation of Piezo1 by mechanical signals promotes bone anabolism. eLife, 8, e49631.

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