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Immulon hbx plates

Manufactured by Thermo Fisher Scientific
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Immulon HBX plates are a type of microtiter plate designed for use in immunoassays. They feature a high-binding surface that helps capture and immobilize proteins or other target molecules effectively. The plates are suitable for various ELISA-based applications, including antibody detection, antigen quantification, and protein-protein interactions.

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Lab products found in correlation

Mouse igg anti β lac clone, by Thermo Fisher Scientific (2 mentions) Nbp1 75130, by Novus Biologicals (2 mentions) 3 3 5 5 tetramethylbenzidine tmb substrate, by Thermo Fisher Scientific (1 mentions) Spectramax m2 plate reader, by Molecular Devices (1 mentions) Haf008, by R&D Systems (1 mentions)

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Immulon 4 HBX ELISA plates (8 citations)

4 protocols using immulon hbx plates

1

Sandwich ELISA for Recombinant β-Lac

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Sandwich ELISAs were performed on 96-well Immulon HBX plates (Thermo Fisher Scientific). A mouse IgG anti-β-Lac antibody (clone: 3E11.G3, Thermo Fisher Scientific) was used to capture recombinant β-Lac and primary murine serum samples were used as primary antibody. A polyclonal anti-mouse IgG HRP antibody was used as detection antibody (Novus Biologicals, Littleton, Colorado, USA). Protein was detected using TMB (3,3′,5,5′-tetramet hylbenzidine) substrate (Thermo Fisher Scientific) and H2SO4 acid quench, and read on a SpectraMax M2 plate reader. Data were analyzed with SoftMax Pro software.
Bourne C.M., Wallisch P., Dacek M., Gardner T., Pierre S., Vogt K., Corless B.C., Bah M.A., Romero Pichardo J., Charles A., Kurtz K.G., Tan D.S, & Scheinberg D.A. (2023). Host-cell Interactions of Engineered T cell Micropharmacies. bioRxiv.
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2

Quantitative Sandwich ELISA for β-Lac

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Sandwich ELISAs were performed on 96-well Immulon HBX plates (Thermo Fisher Scientific). A mouse IgG anti–β-Lac (clone: 3E11.G3, Thermo Fisher Scientific) was used to capture recombinant β-Lac and primary murine serum samples were used as primary antibody. A polyclonal anti-mouse IgG HRP antibody was used as a detection antibody (Novus Biologicals, Littleton, Colorado, USA, NBP1-75130). Protein was detected using TMB (3,3′,5,5′-tetramet hylbenzidine) substrate (Thermo Fisher Scientific, 34028) and H2SO4 acid quench, and then read on a SpectraMax M2 plate reader. Data were analyzed with SoftMax Pro software version 6.2.2.
Bourne C.M., Wallisch P., Dacek M.M., Gardner T.J., Pierre S., Vogt K., Corless B.C., Bah M.A., Romero-Pichardo J.E., Charles A., Kurtz K.G., Tan D.S, & Scheinberg D.A. (2023). Host Interactions with Engineered T-cell Micropharmacies. Cancer Immunology Research, 11(9), 1253-1265.
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3

Sandwich ELISA for β-Lac Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sandwich ELISAs were performed on 96-well Immulon HBX plates (Thermo Fisher Scientific). A mouse IgG anti-β-Lac (clone: 3E11.G3, Thermo Fisher Scientific) was used to capture recombinant β-Lac and primary murine serum samples were used as primary antibody. A polyclonal anti-mouse IgG HRP antibody was used as a detection antibody (Novus Biologicals, NBP1–75130). Protein was detected using 3,3′,5,5′-tetramet hylbenzidine (TMB) substrate (Thermo Fisher Scientific, 34028) and H2SO4 acid quench, and then read on a SpectraMax M2 plate reader. Data were analyzed with SoftMax Pro software version 6.2.2.
Bourne C.M., Wallisch P., Dacek M.M., Gardner T.J., Pierre S., Vogt K., Corless B.C., Bah M.A., Romero-Pichardo J.E., Charles A., Kurtz K.G., Tan D.S, & Scheinberg D.A. (2023). Host Interactions with Engineered T-cell Micropharmacies. Cancer Immunology Research, 11(9), 1253-1265.
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4

Sandwich ELISA for Protein Detection

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Sandwich ELISAs were performed on 96-well Immulon HBX plates (ThermoFisher). A mouse IgG anti-HA antibody was used to capture protein (Invitrogen 26183) and a polyclonal mouse anti-rabbit HRP antibody was used as detection antibody (R&D systems HAF008). Protein was detected using TMB substrate (ThermoFisher 34028) and H2SO4 acid quench and read on a SpectraMax M2 plate reader (Molecular Devices). Data were analyzed with SoftMax Pro software.
Gardner T.J., Lee J.P., Bourne C.M., Wijewarnasuriya D., Kinarivala N., Kurtz K.G., Corless B.C., Dacek M.M., Chang A.Y., Mo G., Nguyen K.M., Brentjens R.J., Tan D.S, & Scheinberg D.A. (2021). Engineering CAR-T Cells to Activate Small-Molecule Drugs in situ. Nature chemical biology, 18(2), 216-225.
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