Mass hunter qualitative analysis b 07.00 metabolom ics 2019 m tool

The LC-MS was used to identify the chemical contents of the extracts. The phytochemical compounds of the methanol extracts were performed using LC-MS followed by [31 (link)]. LC-MS analysis was performed using Agilent spectrometry equipped with a binary pump. The LC-MS was interfaced with Agilent 1290 Infinity LC system coupled to Agilent 6520 accurate-mass Q-TOF mass spectrometer with a dual ESI source. Full-scan mode from m/z 50 to 500 was performed with a source temperature of 125°C.
The column of Agilent zorbax eclipse XDB-C18, narrow-bore 2.1x150 mm, 3.5 microns (P/N: 930990–902) was used with the temperature 30°C for the analysis. A- 0.1% formic acid in water and B -0.1% formic acid in methanol were used as solvents. Isocratic elution was used to supply solvents at a total flow rate of 0.1 mL minutes^-1. MS spectra were collected in both positive and negative ion modes. The drying gas was 300°C, with a 10L min-1 gas flow rate and a 45-psi nebulizing pressure. Before analysis, 1 ml of concentration. sample extracts were diluted with methanol and filtered through a 0.22 m nylon filter. The extracts were injected into the analytical column in 1 μl volume for analysis. The mass fragmentations were discovered using an Agilent mass hunter qualitative analysis B.07.00 (Metabolom-ics-2019.m) tool and a spectrum database for organic chemicals.

LC-MS was used to identify the chemical contents of the extracts. Analysis of the phytochemical compounds in the methanol extracts was performed using LC-MS following the methods in [59 (link)]. LC-MS analysis was performed using an Agilent spectrometer equipped with a binary pump. The LC-MS was interfaced with the Agilent 1290 Infinity LC system coupled to an Agilent 6520 accurate-mass Q-TOF mass spectrometer with a dual ESI source. Full-scan mode from m/z 50 to 500 was performed with a source temperature of 125 °C. An Agilent zorbax eclipse XDB-C18 column, narrow-bore 2.1 × 150 mm, 3.5 microns (P/N: 930990-902), was used at the temperature 30 °C for the analysis. A: 0.1% formic acid in water, and B: 0.1% formic acid in methanol were used as solvents. Isocratic elution was used to supply solvents at a total flow rate of 0.1 mL minutes⁻¹. MS spectra were collected in both positive and negative ion modes. The drying gas was 300 °C, with a 10 mL min⁻¹ gas flow rate and a 45 psi nebulizing pressure. Before analysis, 1 mL of concentrated sample extract was diluted with methanol and filtered through a 0.22 m nylon filter. The extracts were injected into the analytical column in 1 µL volume for analysis. The mass fragmentations were discovered using an Agilent mass hunter qualitative analysis B.07.00 (Metabolom-ics-2019.m) tool and a spectrum database for organic chemicals.