To study the expression of IL-32 isoforms in different cell types, we sorted T-cells, B-cells, NK cells and monocytes from total PBMCs of HIV positive and HIV negative subjects using flow cytometry (BD FACSAria; BD Biosciences). Briefly, cell Surface staining was performed with fluorochrome-conjugated antibodies from BD Biosciences; Pacific Blue™ Mouse Anti-Human CD3 (Clone UCHT1, Cat # 558117), Alexa Fluor® 700 Mouse Anti-Human CD4 (Clone SK3, Cat # 566318), APC-H7 Mouse anti-Human CD8 (Clone SK1, Cat # 560179), APC Mouse Anti-Human CD14 (Clone M5E2, Cat # 555399), PerCP-Cy™5.5 Mouse Anti-Human CD19 (Clone HIB19, Cat # 561295), PE Mouse Anti-Human CD56 (Clone B159, Cat # 561903) and FITC Mouse Anti-Human CD16 (Clone 3G8, Cat # 555406). Average purity of sorted cells was of >95% as determined by post sorting FACS quality control analysis.
Percp cy5.5 mouse anti human cd19
Manufactured by BD
Sourced in United States
PerCP-Cy5.5 Mouse anti-Human CD19 is a fluorochrome-conjugated monoclonal antibody that binds to the CD19 antigen expressed on the surface of human B cells. It is designed for use in flow cytometry applications to detect and analyze B cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Apc mouse anti human cd4, by BD (2 mentions)
Pe mouse anti human cd28, by BD (2 mentions)
V450 mouse anti human cd194 ccr4, by BD (2 mentions)
Alexa fluor 700 mouse anti human cd183 cxcr3, by BD (2 mentions)
Fitc mouse anti human cd8, by BD (2 mentions)
Fitc mouse anti human cd16, by BD (1 mentions)
Pe mouse anti human cd56, by BD (1 mentions)
Facsaria, by BD (1 mentions)
Apc mouse anti human cd14, by BD (1 mentions)
Facscantotm 2, by BD (1 mentions)
4 protocols using percp cy5.5 mouse anti human cd19
1
Profiling IL-32 Isoforms in Immune Cells
2
Multicolor Flow Cytometry Immunophenotyping
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APC Mouse anti-Human CD4 (BD Biosciences), FITC Mouse Anti-Human CD8 (BD), PE Mouse Anti-Human CD28 (BD), V450 Mouse Anti-Human CD194 (CCR4) (BD), Alexa Fluor 700 Mouse anti-Human CD183 (CXCR3) (BD), and PerCP-Cy5.5 Mouse anti-Human CD19 (BD).
3
Multicolor Flow Cytometry Immunophenotyping
4
Flow Cytometric Analysis of Immune Cells
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After the centrifugation and removal of media, cells were surface stained with PerCP-Cy™5.5 mouse anti-human CD19; PE mouse anti-human CD1d; and FITC mouse anti-human CD5 (BD Biosciences, San Diego, CA, USA). After 30 min of incubation in a refrigerator at 4°C, cell suspension was resuspended in PBS and it was then ready for the flow cytometry detection. The samples were read by “BD FACSCantoTM II” flow cytometer (BD Biosciences, San Jose, CA, USA), and data were analyzed by “BD FACSDivaTM V7.0” software (see Figures 1 and 2 ).
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