The largest database of trusted experimental protocols

Nicotine hydrogen bitartrate

Manufactured by Merck Group
Sourced in United States

Nicotine hydrogen bitartrate is a salt compound used in laboratory settings. It serves as a chemical reagent and is commonly used as a precursor in the synthesis of various nicotine-based compounds. The core function of this product is to provide a consistent and reliable source of nicotine for research and development purposes.

Automatically generated - may contain errors

3 protocols using nicotine hydrogen bitartrate

1

Metabolic Profiling of Peach Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols
(–)-Cotinine, 1-hydroxypyrene, nicotine hydrogen bitartrate, sodium acetate, potassium cyanide, chloramine, barbituric acid, and β-glucuronidase/arylsulfatase were obtained from Sigma-Aldrich (St. Louis, MO, USA). Methanol was purchased from Merck (Darmstadt, Germany). All reagents used in this study were of analytical grade. The white-fleshed fruits of Prunus persica (L.) Batsch (Family Rosaceae; peaches) were purchased at the Kyeonggi Dong-Boo Fruit Agriculture Co. (Incheon, Korea), where a voucher specimen (PE20060801) has been deposited.
+ Open protocol
+ Expand
2

Nicotine and Nicotinic Receptor Ligands

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nicotine hydrogen bitartrate (Sigma-Aldrich, St. Louis, MO, USA) and dissolved in 0.9% normal saline (vehicle). pH was adjusted to 7.0 ± 0.5 with 1M sodium hydroxide (J.T. Baker, Center Valley, PA, USA), and solutions were prepared fresh daily and kept in the dark with aluminum foil. Nicotine doses are expressed as free-base [17 (link)] and were injected intraperitoneally (i.p.) 10 minutes prior to testing. Dihydro-β-erythroidine (DHβE) hydrobromide (Tocris, Ellisville, MO, USA) and methyllycaconitine (MLA) citrate salt (Sigma-Aldrich) were dissolved in 0.9% normal saline vehicle, and for pretreatment experiments were injected i.p. 15 minutes prior to nicotine or vehicle i.p. injection, with behavioral testing commencing 10 minutes after last injection. DHβE and MLA doses are expressed as their salts. GTS-21 (Sigma-Aldrich) was dissolved in 0.9% normal saline vehicle and injected i.p. 10 minutes prior to resident-intruder testing. Volumes for i.p. injections were 10 mL/kg for B6 and BALB/c mice, and 5 mL/kg for CD1 mice due to increased body mass.
+ Open protocol
+ Expand
3

Nicotine-Induced Oxidative Stress Attenuation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nicotine hydrogen bitartrate (Sigma Aldrich Co., St. Louis, Mo) was dissolved in 0.9% saline and neutralised to PH 7.2 with NaOH. The treatment was given at a dose of 2.5 mg/kg subcutaneously once daily for 5 days per week/18 weeks [11] . Selection of dose of nicotine was constructed on the previous studies and doses commonly used by other laboratories [11] and the selection of time schedule was based on that more than 20 days of nicotine injection leads to oxidative stress and tissue injury [38, 39] .
Red onion was used because of its reported high antioxidant potentials [32] . It was dressed and frozen (4°C). 100 mL of distilled water per 100 g of onion were added and crushed in a mixing machine. The resultant mixture was squeezed and filtered [32] . The onion extract was given orally once/day in a dose of 50 mg/kg [44] . This dose is nontoxic and has few ill effects [44] .
The NS oil was given at a daily dose of 0.1 g/kg orally. The dosage was chosen based on human NS oil consumption which is equal to 2 g/day; 0.1 g/kg/day was accepted as the corresponding dose in the rat [12] .
All chemicals were purchased from Cap Pharma Company, Egypt.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!