Metformin (D150959), CQ (C6628), MDC (30432), 3-MA (M9281), 4',6-diamidino-2- phenylindole (DAPI; D9542), antibodies against anti-β and LC3, anti-rabbit secondary antibody, anti-mouse secondary antibody and Cy3-labeled rabbit secondary antibody were purchased from Sigma (St. Louis, MO, USA). Antibodies against p-Stat3 (Y705), Stat3, cyclin D1, LC3, PCNA and PARP were purchased from Cell Signaling (Beverly, MA, USA); antibodies against p-AMPK, p-mTOR, Beclin-1, Bcl-2, p62 and Bax were purchased from Santa Cruz (Santa Cruz, CA, USA). Anti-phospho-Histone H3 (Ser10) antibody was purchased from Upstate (Millipore, Billerica, MA, USA). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum were obtained from Gibco (Life Technologies Gibco/BRL, New York, NY, USA). Crystal violet staining and JC-1 were obtained from Beyotime Institute of Biotechnology (Shanghai, China). ApopTag plus peroxidase in situ apoptosis detection kit was obtained from Millipore. AO was obtained from Poly-Sciences (Warrington, PA, USA). Stat3, AMPK, Beclin-1 and Atg5 siRNAs were purchased from Shanghai GenePharma (Shanghai, China). Lipofectamine 2000 was bought from Invitrogen (Carlsbad, CA, USA).
Stat3
Manufactured by GenePharma
Sourced in China
Stat3 is a lab equipment product used in scientific research. It functions as a signal transducer and activator of transcription, playing a role in cellular processes. The core function of Stat3 is to facilitate the transmission of signals from the cell surface to the nucleus, where it can influence gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Beclin1, by GenePharma (2 mentions)
Crystal violet staining, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Cy3 labeled rabbit secondary antibody, by Merck Group (1 mentions)
Atg5 sirna, by GenePharma (1 mentions)
Anti rabbit secondary antibody, by Merck Group (1 mentions)
Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions)
Anti mouse secondary antibody, by Merck Group (1 mentions)
Beclin 1, by Santa Cruz Biotechnology (1 mentions)
Apoptag plus peroxidase in situ apoptosis detection kit, by Merck Group (1 mentions)
Bcl2 associated x (bax), by Santa Cruz Biotechnology (1 mentions)
Bcl 2, by Santa Cruz Biotechnology (1 mentions)
Control sirna, by GenePharma (1 mentions)
H1299, by Genechem (1 mentions)
Oligofectamine 2000, by Thermo Fisher Scientific (1 mentions)
5 protocols using stat3
1
Autophagy Modulation in Cancer Therapeutics
2
Transfection of Autophagy Regulators
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The siRNAs to Atg5, Beclin-1, Stat3, AMPK or control siRNA were all purchased from Shanghai GenePharma. Cells were transfected with siRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. Cells were incubated for 48 h before further treatment.
3
Silencing Survivin and STAT3 in GBM Cells
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Cells were transfected twice with the same siRNA at a 24-h interval with lipofectamine 2000 (Invitrogen). The final concentration of siRNAs was 20 nM. Sequences for the siRNAs used were the following: survivin, 5′-GCATTCGTCCGGTTGCGCT-3′; STAT3, 5′-GUUCAUCUGUGUGACACCATT-3′; nontargeting siRNA controls, 5′-UUCUCCGAACGUGUCACGUTT-3′ (Genepharma, Shanghai, China). The STAT3 cDNA (KIAA1524) was purchased from the Addgene plasmid repository (http://www.addgene.org/ ). After transfection, U251 and U87 cells were cultured in the presence of G418 (0.8 mg/mL) for 8 weeks to select for stable transfectants. GBM cells that stably expressed STAT3 were used for treatment with YM155 and radiation.
4
siRNA Synthesis for STAT3 Inhibition
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5
Modulating α5-nAChR Expression in Lung Cancer
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siRNAs for CHRNA5 (encoding α5‐nAChR), Jab1, Stat3 and the negative control were obtained from GenePharma. Transient transfection of A549 and H1299 cells was performed using the Oligofectamine 2000 (Invitrogen) protocol and 5‐nmol siRNAs in RPMI supplemented with 10% FBS and no penicillin or streptomycin as described previously.18 Human CHRNA5 cDNA was subcloned into a pGV‐puro lentiviral vector containing the puromycin resistance to establish stable A549 and H1299 (Genechem) cell lines to induce α5‐nAChR overexpression (marked as α5‐nAChR+). Stable A549 and H1299 cells were selected by incubating cells in medium supplemented with puromycin for 48 hours after transfection.
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