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Aspp2

Manufactured by Abcam
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ASPP2 is a protein that plays a role in regulating cellular processes. It is expressed in various tissues and is involved in apoptosis, cell cycle progression, and other cellular functions. The product provides a reliable source of this protein for use in research applications.

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Lab products found in correlation

Hrp conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Gfp antibody, by Proteintech (2 mentions) Quickhange mutagenesis, by Agilent Technologies (2 mentions) Repoman, by Abcam (2 mentions) Pp1g antibodies, by Santa Cruz Biotechnology (2 mentions) Py701 stat1, by Cell Signaling Technology (1 mentions) Notch1, by Santa Cruz Biotechnology (1 mentions) Flag tag (flag), by Santa Cruz Biotechnology (1 mentions) β catenin, by Merck Group (1 mentions) E cad, by Cell Signaling Technology (1 mentions) Fih 1, by Santa Cruz Biotechnology (1 mentions) Gadd45α, by Santa Cruz Biotechnology (1 mentions) Hdac1, by Cell Signaling Technology (1 mentions) Stat1, by Cell Signaling Technology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions)

3 protocols using aspp2

1

GFP-tagged Protein Phosphatase Constructs

Cited 1 time
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The pEGFP(C1)-PP1α and pEGFP(C1)-PP1γ plasmids were previously described14 (link) and are available through Addgene (plasmids #44224 and #44225). The C-terminal truncation mutant pEGFP(C1)-PP1γΔ299–323 was derived from the full-length construct by PCR-based insertion of a premature STOP codon. The C-terminal chimera pEGFP(C1)-PP1γ(1–298)PP1α(299–330) was derived by PCR-based insertion of a SalI restriction site into the full-length construct at the relevant site, followed by digestion and ligation of the PP1α C-terminal coding sequence. The pEGFP(C1)-RRP1B and pEGFP(C1)-RRP1BRAxA (V684A/F686A) plasmids were previously described.15 (link) PP1 and RRP1B point mutants were derived using Quickhange mutagenesis (Agilent Technologies) and sequence confirmed. Antibodies that recognize RRP1B, RepoMan and ASPP2 were obtained from Abcam, PP1g antibodies were from Santa Cruz and GFP-antibodies from proteintech®. HRP-conjugated secondary antibodies were from ThermoFisher.
Srivastava G., Bajaj R., Kumar G.S., Gaudreau-Lapierre A., Nicolas H., Chamousset D., Kreitler D., Peti W., Trinkle-Mulcahy L, & Page R. (2022). The ribosomal RNA processing 1B:protein phosphatase 1 holoenzyme reveals non-canonical PP1 interaction motifs. Cell reports, 41(9), 111726.
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2

Comprehensive Western Blot Antibody Panel

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Western blots were performed as described previously (26 ). The following antibodies were used: FIH-1, Grb2, tubulin, GAPDH, Notch1, GADD45α, FLAG (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), p63α, E-cad, STAT1, pY701-STAT1, Plectin1, HDAC1, (Cell Signaling Technology, Danvers, MA, USA), ASPP2 (Abcam, Cambridge, United Kingdom), and β-catenin (Sigma-Aldrich Corp., St Louis, MO, USA).
Kaplan N., Dong Y., Wang S., Yang W., Park J.K., Wang J., Fiolek E., White B.P., Chandel N.S., Peng H, & Lavker R.M. (2019). FIH-1 engages novel binding partners to positively influence epithelial proliferation via p63. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(1), 525-539.
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3

GFP-tagged Protein Phosphatase Constructs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The pEGFP(C1)-PP1α and pEGFP(C1)-PP1γ plasmids were previously described14 (link) and are available through Addgene (plasmids #44224 and #44225). The C-terminal truncation mutant pEGFP(C1)-PP1γΔ299–323 was derived from the full-length construct by PCR-based insertion of a premature STOP codon. The C-terminal chimera pEGFP(C1)-PP1γ(1–298)PP1α(299–330) was derived by PCR-based insertion of a SalI restriction site into the full-length construct at the relevant site, followed by digestion and ligation of the PP1α C-terminal coding sequence. The pEGFP(C1)-RRP1B and pEGFP(C1)-RRP1BRAxA (V684A/F686A) plasmids were previously described.15 (link) PP1 and RRP1B point mutants were derived using Quickhange mutagenesis (Agilent Technologies) and sequence confirmed. Antibodies that recognize RRP1B, RepoMan and ASPP2 were obtained from Abcam, PP1g antibodies were from Santa Cruz and GFP-antibodies from proteintech®. HRP-conjugated secondary antibodies were from ThermoFisher.
Srivastava G., Bajaj R., Kumar G.S., Gaudreau-Lapierre A., Nicolas H., Chamousset D., Kreitler D., Peti W., Trinkle-Mulcahy L, & Page R. (2022). The ribosomal RNA processing 1B:protein phosphatase 1 holoenzyme reveals non-canonical PP1 interaction motifs. Cell reports, 41(9), 111726.
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