Full blood count and other hematological traits were measured using the Coulter ACT5 Diff CP hematology analyzer. The following information was output: white cell count, red cell count, haemoglobin (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC), mean platelet volume (MPV) and platelet count.
Act5 diff cp
Manufactured by Beckman Coulter
The ACT5 Diff CP is a compact, fully automated hematology analyzer that provides a complete blood count (CBC) with a 5-part differential. The device is designed to deliver accurate and reliable results for routine clinical laboratory testing.
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Lab products found in correlation
4 protocols using act5 diff cp
1
Hematological Traits Analysis
2
Blood Cell Characterization in HIV
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Full blood count and CD4+ cell counts were done using the Coulter ACT 5Diff CP and Facscalibur, as per standard operating procedures at the ISS clinic. Reference ranges as determined for the HIV population were used.8 (link) Thrombocytopenia was regarded as platelet count less than 150×109/L, anemia was defined as hemoglobin concentration less than 12 g/L for adult females and less than 13 g/dL for adult males, and leucopenia was considered as white blood cell (WBC) count less than 2.75×109/L. Thin blood films were prepared, stained by Giemsa and examined for anemia typing based on: a) size of red blood cells (normocytic, microcytic and macrocytic) and b) hemoglobin content (normochromic and hypochromic). Pancytopenia was considered if all the three blood cells were below their minimum for a reference range. We ensured strict adherence to the standard operating procedures, the hematology analyzer was well maintained and controls were run daily before use. A hematology bench aid was used to ascertain cell features of a comprehensive film comment.
3
Hematology Analysis and Trait Normalization
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Fifteen FBC traits were measured using the Beckman Coulter ACT5 Diff CP hematology analyzer (Table 1 ). We carried out the inverse normal transformation of each trait residual. First, we obtained residuals after the regression of each trait on age, age2, and sex. We then inverse normally transformed the residuals for GWAS analysis.
4
Evaluating Vaccine Safety in Baboons
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To determine vaccine safety and tolerability, venous EDTA blood was collected every four weeks prior to vaccinations from all baboons after sedation with a mixture of 10 mg/kg Ketamine HCL and 0.5 mg/kg xylazine (Agrar, Holland). Haematological indices were analyzed using Beckman Coulter ACT 5 Diff. CP (USA). The analyses included complete blood cell counts, hematocrit, hemoglobin, mean corpuscular volume, platelet counts, erythrocyte counts and leukocyte counts. Sera were also collected for determination of clinical chemistry parameters using Humalyzer 2000 (Human, Germany) machine. The concentrations of creatinine phosphokinase (CK-MB), total bilirubin, direct bilirubin, alanine aminotransferase (ALAT/GPT), aspartate aminotransferase (ASAT/GOT), urea, and total protein concentrations were measured according to the manufacturer's instructions.
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