Luxeptinib was provided by Aptose Biosciences. Ibrutinib (#HY-10997) was purchased from MedChem Express. Goat anti-human IgM (#109-005-043) was purchased from Jackson ImmunoResearch. Human lymphoma cell lines SU-DHL-6 (#CRL-2959), JeKo-1 (#CRL-3006), RL (#CRL-2261) and Fetal Bovine Serum (#30–2020) were obtained from ATCC. SU-DHL-6 is a human lymphoblast-like cell line, JeKo-1 is a mantle cell lymphoma cell line and RL is a human non-Hodgkin’s lymphoma B cell line. RPMI-1640 medium (#11875–093) and penicillin/streptomycin (#15070063) were purchased from Thermo Fisher Scientific. Antibodies against p-BTK (Y223) (#87141), BTK (#56044), p-PLCγ2 (#3871), p-SYK (#2710), SYK (#80460), p-BLNK (#3601), BLNK (#36438), p-CD79A (#5173), p-LYN/LCK/HCK/BLK (#70926), p-LYN (Y507) (#2731), LYN (#2796, #4576), pSrc family (#6943) and GAPDH (#2118) were purchased from Cell Signaling Technology. Phospho-BTK (Y551) (#ab40770) was purchased from Abcam. PLCγ2 (#sc-5283) and CD79A (#sc-20064) were purchased from Santa Cruz Biotechnology. All other reagents and chemicals used were of analytical grade and were obtained from Sigma-Aldrich or Thermo Fisher Scientific.
P blnk
Manufactured by Cell Signaling Technology
P-BLNK is a primary antibody that recognizes B-cell linker protein (BLNK) phosphorylated at tyrosine 84. BLNK is an adaptor protein that plays a critical role in B-cell receptor signaling.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Ibrutinib, by MedChemExpress (1 mentions)
P syk, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions)
P plcγ2, by Cell Signaling Technology (1 mentions)
Goat anti human igm, by Jackson ImmunoResearch (1 mentions)
Su dhl 6, by American Type Culture Collection (1 mentions)
Jeko 1, by American Type Culture Collection (1 mentions)
P btk y223, by Cell Signaling Technology (1 mentions)
P cd79a, by Cell Signaling Technology (1 mentions)
P lyn y507, by Cell Signaling Technology (1 mentions)
Plcγ2, by Santa Cruz Biotechnology (1 mentions)
Cd79a, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Lysis buffer, by Cell Signaling Technology (1 mentions)
Sds page, by Bio-Rad (1 mentions)
Plcγ2, by Cell Signaling Technology (1 mentions)
Nfatc1, by Cell Signaling Technology (1 mentions)
2 protocols using p blnk
1
Western Blot Analysis of BCR Signaling
2
Osteoclast Signaling Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total lysates of human osteoclast precursor cells were prepared from the lysis buffer (Cell Signaling Technology). Total lysates were loaded on 4–12% SDS-PAGE (Bio-Rad), and then western blot analysis was performed using antibodies, pBtk Y223 (Novus Biologicals), pLyn Y396 (Gene Tex), Btk, Lyn, pGab2 Y452, Gab2, pPLCγ2 Y759, PLCγ2, pBLNK, BLNK, NFATc1 (Cell Signaling Technology).
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