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2 protocols using phenylbis 2 4 6 trimethylbenzoyl phosphine oxide

1

Photopolymerized Hydrogel Nanoparticle Synthesis

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Glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EDMA), 1-dodecanol, 2,2-dimethoxy-2-phenylacetophenone (DMPA), poly (ethylene glycol) diacrylate (PEGDA, MW 250), Tris hydrochloride, 3-(trimethoxysilyl)propyl methacrylate, dimethyl sulfoxide (DMSO), phenylbis (2,4,6 trimethylbenzoyl) phosphine oxide (Irgacure 819), Amicon ultra 0.5-mL centrifugal filters (10 and 50 kDa cutoff), boric acid, sodium bicarbonate, and sodium carbonate were obtained from ThermoFisher (St. Louis, MO). Alexa Fluor 532 (carboxylic acid, succinimidyl ester) and Tris base were purchased from Fisher Scientific (Fair Lawn, NJ). Solutions were made using deionized water (18.3 MΩ) filtered by a Barnstead EASY-pure UV/UF system (Dubuque, IA). Isopropyl alcohol (IPA) was from Macron (Center Valley, PA). Cyclohexanol was obtained from Spectrum (New Brunswick, NJ). 2-nitrophenyl phenyl sulfide (NPS) came from TCI (Portland, OR). Amicon ultra-4 centrifugal filters (15 mL, 10 and 30 kDa cutoff), and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were purchased from EMD Millipore (Billerica, MA). Glass slides for 3D printing were purchased from VWR (Radnor, PA).
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2

Photopolymer Resin Synthesis and Characterization

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Clear photopolymer standard resin (a mixture of proprietary acrylic monomers and oligomers and phenylbis (2,4,6-trimethyl benzoyl)-phosphine oxide as photoinitiator) was purchased from XYZprinting, Inc. (XYZprinting, New Taipei City, Taiwan). phenylbis (2,4,6-trimethyl benzoyl)-phosphine oxide and silver perchlorate (AgClO4) were purchased from Alfa Aesar (Thermo Fisher, Kendal, Germany). Methanol, N,N-dimethylformamide (DMF), and isopropanol (IPA) were purchased from Scharlau (Scharlab, Sentmenat, Spain). All products were used as received.
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