Htb 80

Pancreatic cell lines hTERT-HPNE (ATCC^® CRL-4023™), Capan-2 (ATCC^® HTB-80™) and AsPC-1 (ATCC^® CRL-1682™) were obtained from ATCC (Manassas, VA, USA). Each cell line was maintained in specialized medium according to the supplier’s specifications.

The human pancreatic adenocarcinoma AsPC-1 and Capan-2 cell lines were cultured in RPMI-1640 medium supplemented with 10% FBS, 100 units/ml penicillin and 100 μg/ml streptomycin. Panc-1 and MIA Paca-2 cell lines were cultured in DMEM medium supplemented with 10% FBS, 100 units/ml penicillin and 100 μg/ml streptomycin. The cells were cultured and maintained as previously described [21 (link)]. We obtained AsPC-1 cells (ATCC® CRL-1682™) in 2010, MIA Paca-2 (ATCC® CRL-1420™) cells and Panc-1 (ATCC® CRL-1469™) cells in 2016 and Capan-2 cells (ATCC® HTB-80™) in 2019. All cell lines were obtained from ATCC (Manassas, VA, USA). After obtaining cells from ATCC, cells were frozen at low passage (p3-p5), and then thawed and used at passage 10 or lower. These cell lines were tested for mycoplasma contamination at regular intervals. we did not authenticate these cell lines.