Heat-inactivated SARS-CoV-2 (strain: 2019-nCoV/USA-WA1/2020) was purchased from ATCC (Manassas, VA, USA; cat. no. ATCC VR-1986HK), the N-terminally His-tagged recombinant SARS-CoV-2 spike protein S1 subunit (region Val16–Gln690) from RayBiotech (Peachtree Corners, GA, USA) recombinant human syndecan-4 from R&D Systems (Minneapolis, MN, USA; cat. no. 2918-SD) and recombinant human ACE2 from Abcam (Cambridge, UK; ab151852). Peptide inhibitors utilized for the studies were purchased from either Bachem (Bubendorf, Switzerland; DX600) or Genscript (Leiden, The Netherlands; SPRRAR and WQPPRARI). SARS-CoV-2 pseudovirus-RFP (SARS-CoV-2 PSV-RFP), recombinant pseudotyped lentiviral particles containing SARS-CoV-2 spike protein and encoding red fluorescent protein (RFP) in their lentiviral genome was purchased from GeneMedi (Shanghai, China; cat. no. GM-2019nCoV-PSV01).
Recombinant human syndecan 4
Manufactured by R&D Systems
Sourced in United States
Recombinant human syndecan-4 is a protein that is produced using recombinant DNA technology. Syndecan-4 is a member of the syndecan family of cell surface proteoglycans. It functions as a co-receptor for growth factors and cell adhesion molecules.
Automatically generated - may contain errors
Lab products found in correlation
Sebacic acid, by Merck Group (2 mentions)
Glycerol, by Thermo Fisher Scientific (2 mentions)
Wqpprari, by GenScript (1 mentions)
Human cxcl12 sdf 1α, by R&D Systems (1 mentions)
Lactate dehydrogenase ldh activity assay kit, by Merck Group (1 mentions)
Human complement c3 elisa kit, by Abcam (1 mentions)
3 protocols using recombinant human syndecan 4
1
Establishing SARS-CoV-2 Protein Interaction Assays
2
Silk Fibroin and PGS Scaffold Synthesis
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Silk fibroin protein was extracted from raw silk (Haian Silk Company, Nantong, China) using an aqueous solvent processing method according to published protocols [14 (link)]. PGS prepolymer was synthesized from glycerol (Fisher Scientific) and sebacic acid (Sigma-Aldrich) following published methods [15 (link)]. Type I collagen from calfskin was purchased commercially (Elastin Products Company, Inc.). Recombinant human syndecan-4 and human CXCL12/SDF-1α were purchased from R&D Systems.
3
Biomaterial Synthesis and Characterization
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Poly(glycerol sebacate) (PGS) prepolymer was synthesized from sebacic acid (Sigma-Aldrich) and glycerol (Fisher Scientific) using a published protocol [38] . Type I collagen was purchased commercially (Elastin Products Company, Inc). Silk fibroin was extracted from raw silk (Haian Silk Company, Nantong, China) according to published methods [39] . Recombinant human syndecan-4 was obtained commercially (R&D Systems). The human complement C3 ELISA kit and the human complement C3a des Arg ELISA kit were purchased from Abcam. The lactate dehydrogenase (LDH) activity assay kit was purchased from Sigma-Aldrich. ePTFE vascular grafts were obtained from GORE-TEX® (Newark, Delaware). Bovine pericardial patch for valve replacement (BPV) was obtained from Edwards Lifesciences Corp. (Irvine, CA).
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