Maxwell tissue dna kit

Manufactured by Promega

Sourced in United States

The Maxwell Tissue DNA Kit is a laboratory consumable product designed for the extraction and purification of genomic DNA from a variety of tissue samples. The kit utilizes magnetic bead technology to provide a simple and efficient method for isolating high-quality DNA for downstream analytical applications.

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Lab products found in correlation

Miseq instrument, by Illumina (2 mentions) Fastq files, by Illumina (1 mentions) Maxwell 16 research system, by Promega (1 mentions) Maxwell 16 research instrument system, by Promega (1 mentions) Miseq platform, by Illumina (1 mentions) Ffpe dna mini kit, by Qiagen (1 mentions) Maxwell instrument, by Promega (1 mentions)

Spelling variants of this product

Maxwell 16 FFPE Tissue LEV DNA puri cation kits (3 citations) Maxwell kits (2 citations) Maxwell 16 Tissue and Blood DNA purification kits (2 citations)

7 protocols using maxwell tissue dna kit

Gut Microbiome Profiling in Rats

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Faeces samples were collected from rats at the end of the study and used to purify total genomic DNA using a cDNA extraction Maxwell DNA Tissue Kit with an automated Maxwell^® 16 Research System instrument (Promega, Madison, WI, USA). The final equimolar pool was sequenced on the Illumina MiSeq platform. PCR reactions and 16S sequencing were performed at the Molecular Research LP (MRDNA, Shallowater, TX, USA). The MiSeq instrument (Illumina, San Diego, CA, USA) was used for sequencing the 16S amplicons following the manufacturer’s instructions at MRDNA described by Garcia-Mazcorro et al. [41 (link)] with slight modifications. Raw 16S data were obtained from Illumina’s basespace as FASTQ files and analysed using the QIIME 2 pipeline using the procedure as described in the moving pictures tutorial (https://docs.qiime2.org/2018.11/tutorials/moving-pictures/ accessed on: 15 November 2021).

Novak J., Butorac K., Leboš Pavunc A., Banić M., Butorac A., Lepur A., Oršolić N., Tonković K., Bendelja K., Čuljak N., Lovrić M., Šušković J, & Kos B. (2021). A Lactic Acid Bacteria Consortium Impacted the Content of Casein-Derived Biopeptides in Dried Fresh Cheese. Molecules, 27(1), 160.

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Rat Gut Microbiome Analysis by 16S Sequencing

Cited 1 time

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Rat faecal samples were collected at the end of the study and the total genomic DNA was extracted using a Maxwell DNA Tissue Kit with automated extraction platform, Maxwell^® 16 Research System instrument (Promega, Madison, USA). The final equimolar pool was sequenced on the Illumina MiSeq platform using 341F (5′-CCTACGGGNGGCWGCAG-3′) and 518R (5′-ATTACCGCGGCTGCTGG-3′) primers. PCR reactions and 16S sequencing were performed at the Molecular Research LP (MRDNA, Shallowater, Texas, USA). The MiSeq instrument (Illumina) was used for sequencing the 16S amplicons following the manufacturer’s instructions at MRDNA described by Garcia-Mazcorro et al. [21 (link)] with slight modifications. Raw 16S data were obtained from Illumina’s basespace as FASTQ files and analysed with the QIIME 2 pipeline using the procedure as described in the ‘moving pictures’ tutorial (https://qiime2.org/).

Butorac K., Banić M., Novak J., Leboš Pavunc A., Uroić K., Durgo K., Oršolić N., Kukolj M., Radović S., Scalabrin S., Žučko J., Starčević A., Šušković J, & Kos B. (2020). The functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit. Microbial Cell Factories, 19, 106.

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Genotyping for Calpain-2 and LDL Receptor Genes

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Mouse genotypes were confirmed by PCR. DNA was isolated from tail snips using a Maxwell tissue DNA kit (Cat# AS1030, Promega, Madison, WI). Calpain-2f/f genotyping used the following primers: 5’-ATAGCTCCTGTGTATCAGGCACAGAGCTGG and 5’- CTCTGGTCAGGTCTTAGTTCCCAGAGGATG. Resultant wild-type, and flox allele bands were 290, and 430 bp, respectively (Figure IA in the Data Supplement). Cre+ genotyping used the following primers: 5’-ACCTGAAGATGTTCGCGATT and 5’-CGGCATCAACGTTTTCTTTT. The resultant Cre+ hemizygous allele PCR product was 182 bp and no product for non-transgenic mice. The IL-2 gene was used as an internal control for Cre+ genotyping using the following primers: 5’-CTAGGCCACAGAATTGAAAGATCT and 5’-GTAGGTGGAAATTCTAGCATCATCC. The resultant product was 324 bp (Figure IB in the Data Supplement). LDL receptor genotyping was performed as described previously.^{14 (link)}

Muniappan L., Okuyama M., Javidan A., Thiagarajan D., Jiang W., Moorleghen J.J., Yang L., Balakrishnan A., Howatt D.A., Uchida H.A., Saido T.C, & Subramanian V. (2021). Inducible Depletion of Calpain-2 Mitigates Abdominal Aortic Aneurysm in Mice. Arteriosclerosis, thrombosis, and vascular biology, 41(5), 1694-1709.

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Genotyping of miR-146a Knockout Mice

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Mouse genotypes were confirmed by PCR^{42 (link),43 (link)}. Genomic DNA was isolated from tail snips using a Maxwell tissue DNA kit (Cat# AS1030, Promega, Madison, WI). miR-146a genotyping was performed using the following primers: 5′-GCTTATGAACTT GCCTATCTGTG-3′ and 5′-CAGCAGTTCCACGCTTCA-3′. PCR of wild-type and disrupted alleles generated amplicons of 350 bp and 159 bp, respectively. A representative gel of miR-146a+/+ and −/− amplicons is shown in the Fig. 1C ^{42 (link),43 (link)}.

Javidan A., Jiang W., Okuyama M., Thiagarajan D., Yang L., Moorleghen J.J., Muniappan L, & Subramanian V. (2019). miR-146a Deficiency Accelerates Hepatic Inflammation Without Influencing Diet-induced Obesity in Mice. Scientific Reports, 9, 12626.

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Mouse Genotyping by PCR Analysis

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Mouse genotypes were confirmed by PCR. DNA was isolated from tail snips or BM-derived cells using a Maxwell tissue DNA kit (Cat# AS1030, Promega, Madison, WI). CAST-Tg genotyping was performed using the following primers: 5′-GTTGGCTTAGGCTGCTTTTCGT-3′ and 5′-CCAGACTCCGTGA CACCCCTT-3′. The resultant CAST-Tg PCR product was 518 base pairs (bp) and no product for non-transgenic mice. The IL-2 gene was used as an internal control for CAST-Tg genotyping using the following primers: 5′-CTAGGCCACAGAATTGAAAGATCT and 5′-GTAGGTGGAAATTCTAGCATCATCC. The resultant product was 324 bp (Figure SI in the online-only Data Supplement).

Muniappan L., Javidan A., Jiang W., Mohammadmoradi S., Moorleghen J.J., Katz W.S., Balakrishnan A., Howatt D.A, & Subramanian V. (2017). Calpain Inhibition Attenuates Adipose Tissue Inflammation and Fibrosis in Diet-induced Obese Mice. Scientific Reports, 7, 14398.

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Genotyping Mouse Calpain-2 and Cre Alleles

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Mouse genotypes were confirmed by PCR. DNA was isolated from tail snips using a Maxwell tissue DNA kit (Cat# AS1030, Promega, Madison, WI). Calpain-2f/f genotyping used the following primers: 5'-ATAGCTCCTGTGTATCAG GCACAGAGCTGG-3' and 5'-CTCTGGTCAGGTCTTAGTTCCCAGAGGATG -3'. Resultant wild-type, and flox allele bands were 290, and 430 bp, respectively (Supplemental Figure S1A). Cre+ genotyping used the following primers: 5'-ACCTGAAGATGTTCGCGATT and 5'-CGGCATCAACGTTTTCTTTT. The resultant Cre+ hemizygous allele PCR product was 182 bp and no product for non-transgenic mice. The IL-2 gene was used as an internal control for Cre+ genotyping using the following primers: 5'-CTAGGCCACAGAATTGAAAGAT CT and 5'-GTAGGTGGAAATTCTAGCATCA TCC. The resultant product was 324 bp (Supplemental Figure S1B). LDL receptor genotyping was performed as described previously.( 44)

Muniappan L., Okuyama M., Javidan A., Thiagarajan D., Jiang W., Moorleghen J.J., Yang L., Balakrishnan A., Howatt D.A., Uchida H.A., Saido T.C., & Subramanian V. (2020). Inducible Depletion of Calpain-2 Mitigates Abdominal Aortic Aneurysm in Mice.

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Comprehensive DNA Extraction and USP8 Sequencing

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DNA extraction from the 24 frozen tumours was performed using a Maxwell Instrument and Maxwell Tissue DNA Kit (Promega). The FFPE DNA mini kit (Qiagen) was used for DNA extraction from the 51 FFPE specimens. Exon 14 of USP8 containing the mutational hotspot was amplified by PCR and sequenced as previously described (Perez-Rivas et al. 2015 (link), 2017) . Chromatograms were analysed with the Mutation Surveyor software version v4.09 (Soft Genetics).

Albani A., Perez-Rivas L.G., Tang S., Simon J., Lucia K.E., Colón-Bolea P., Schopohl J., Roeber S., Buchfelder M., Rotermund R., Flitsch J., Thorsteinsdottir J., Herms J., Stalla G., Reincke M, & Theodoropoulou M. (2022). Improved pasireotide response in USP8 mutant corticotroph tumours in vitro. Endocrine-related cancer, 29(8).

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