Lpl activity assay kit

Manufactured by Cell Biolabs

Sourced in United States

The LPL Activity Assay Kit is a laboratory tool designed to quantitatively measure the activity of the enzyme lipoprotein lipase (LPL) in biological samples. The kit provides a colorimetric-based assay that allows for the detection and analysis of LPL activity.

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Lab products found in correlation

Anti sirt1, by Santa Cruz Biotechnology (1 mentions) Resveratrol rsv, by Merck Group (1 mentions) Hf diet, by Research Diets (1 mentions) F 12k medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) β actin protein, by Cell Signaling Technology (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)

5 protocols using lpl activity assay kit

Serum Lipoprotein Lipase Activity Assay

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Serum LPL activity (units ml⁻¹) was measured using an LPL Activity Assay Kit (Cell Biolabs, Inc., San Diego, CA, USA) according to the manufacturer’s instructions.

, & Okuda T. (2019). A low-carbohydrate ketogenic diet induces the expression of very-low-density lipoprotein receptor in liver and affects its associated metabolic abnormalities. NPJ Science of Food, 3, 25.

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LPL Quantification in Transfected COS-7 Cells

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Post‐heparin transfected COS‐7 cell culture medium was harvested and centrifuged at 4°C for 20 minutes at 12 000 g. The remaining precipitated cells were washed with PBS and then solubilized in 80 μL cell lysis buffer (Tiangen Biotechnology Co., Ltd.). The cell lysates were centrifuged at 4°C for 20 minutes at 12 000 g, and the supernatant was stored at −80°C. LPL mass and activity were determined by Human LPL ELISA kit (Shanghai Xinfan Biotechnology Co., Ltd.) and LPL activity assay kit (Cell Biolabs, INC.), respectively, by using M5 full‐band multifunctional enzyme label instrument (Molecular Devices). In addition, for LPL activity assay, the parameters were set to 485/520 nm and filter set with cut‐off of 495 nm.

Han P., Wei G., Cai K., Xiang X., Deng W.P., Li Y.B., Kuang S., Dong Z., Zheng T., Luo Y., Liu J., Guan Y., Li C., Dey S.K., Liao Z, & Banerjee S. (2020). Identification and functional characterization of mutations in LPL gene causing severe hypertriglyceridaemia and acute pancreatitis. Journal of Cellular and Molecular Medicine, 24(2), 1286-1299.

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Evaluating Adipogenic Regulators in Cells

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Rosiglitazone (ROSI), GW9662, resveratrol (RSV), and 6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide (EX-527) were from Sigma (St. Louis, MO). Anti-LPL antibody was from GeneTex (Irvine, CA). The LPL activity assay kit was from Cell Biolabs, Inc. (San Diego, CA). Antibodies for PPARγ, fatty acid synthase (FASN), and β-actin proteins were from Cell Signaling Technology, Inc. (Danvers, MA). Anti-SIRT1, CCAAT/enhancer binding protein-α (C/EBPα), and SREBP1c antibodies were from Santa Cruz Biotechnology (Dallas, TX). FBS, penicillin-streptomycin, DMEM, and F-12K medium were from Invitrogen (Carlsbad, CA). HF diet (60 kcal% from fat, 20 kcal% from protein, 20 kcal% from carbohydrate, energy density 5.24 kcal/g; catalog number D12492) was from Research Diets, Inc. (New Brunswick, NJ). Regular chow (17 kcal% from fat, 25 kcal% from protein, 58 kcal% from carbohydrate, energy density 3.1 kcal/g; catalog number 7912) was from Harlan Laboratories (Madison, WI).

Qiao L., Guo Z., Bosco C., Guidotti S., Wang Y., Wang M., Parast M., Schaack J., Hay WW J.r., Moore T.R, & Shao J. (2015). Maternal High-Fat Feeding Increases Placental Lipoprotein Lipase Activity by Reducing SIRT1 Expression in Mice. Diabetes, 64(9), 3111-3120.

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Hepatic VLDL-Triglyceride Secretion and LPL Activity

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Hepatic VLDL-triglyceride secretion and plasma lipid clearance were measured as previously described (21 (link)). LPL activity was assessed in pre- and post-heparin plasma using the LPL Activity Assay Kit from Cell Biolabs, Inc. according to the manufacturer's instructions.

Goedeke L., Bates J., Vatner D.F., Perry R.J., Wang T., Ramirez R., Li L., Ellis M.W., Zhang D., Wong K.E., Beysen C., Cline G.W., Ray A.S, & Shulman G.I. (2018). Acetyl-CoA Carboxylase Inhibition Reverses NAFLD and Hepatic Insulin Resistance but Promotes Hypertriglyceridemia in Rodents. Hepatology (Baltimore, Md.), 68(6), 2197-2211.

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Fluorometric Assay for LPL Activity

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Serum samples were used to determine LPL activity using a fluorometric LPL Activity Assay Kit (Cell Biolabs, San Diego, CA, USA) according to the manufacturer's protocols.

Mao H., Lockyer P., Li L., Ballantyne C.M., Patterson C., Xie L, & Pi X. (2017). Endothelial LRP1 regulates metabolic responses by acting as a co-activator of PPARγ. Nature Communications, 8, 14960.

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