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Mouse ifn gamma elisa kit

Manufactured by MultiSciences Biotech

The Mouse IFN-gamma ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the detection and quantification of mouse interferon-gamma (IFN-gamma) in biological samples such as cell culture supernatants, cell lysates, and plasma.

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2 protocols using mouse ifn gamma elisa kit

1

Expression and Quantification of His-tagged Protein

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Expression levels of E317Ab containing a His-tag in culture supernatants or mouse serum were measured using a His-tag ELISA detection kit (GenScript, cat. no. L00436). Mouse serum levels of IL-6, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and IgG were detected using the LEGEND Max™ mouse IL-6 ELISA kit (BioLegend, cat. no. 431307), mouse TNF-α ELISA kit (MultiSciences, cat. no. 70-EK282/3-96), mouse IFN-gamma ELISA kit (MultiSciences, cat. no. 70-EK280/3-96), and the mouse IgG ELISA kit (MultiSciences, cat. no. 70-EK271-96) according to manufacturer’s instructions.
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2

Tumor-derived Chemokine Profiling

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Supernatants were harvested from single-cell suspensions of tumor tissues, cultured and stimulated with a cell stimulation cocktail (including protein transport inhibitors) (00-4975-03, eBioscience) for 4-6 h at 37 °C in RPMI 1640 medium, followed by centrifugation at 2000 rpm for 10 min for chemokine analysis using ELISA kits (Mouse CXCL9/MIG ELISA Kit, 70-EK2143/2-48, MULTI SCIENCE; Mouse IFN-gamma ELISA Kit, 70-EK280/3-96, MULTI SCIENCE; Mouse lymphotactin/XCL1 ELISA Kit, SEK50677, Sino Biological) according to the manufacturer’s instructions.
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