Acrylamide bis solution

Fetal Bovine Serum (Bio-Channel, BC-SE-FBS07), DMEM/F-12 (BiologicalIndustries, 01-170-1A), EDTA-Trypsin (Biosharp, BL512A), EndoFectin™Max Reagent (GeneCopoeia, EF013), Cell Value Added-Toxicity Assay Kit (Biosharp, BS350B), PA (Aladdin, S161420), OA (Maclean, S817542), ORO Staining Kit (Solarbio, G1262), BlazeTaq™ SYBR®Green Mix (GeneCopoeia, QP031), Dnase QP031), BCA Protein Reagent (P0009-1, P0009-1), Hematoxylin (Solarbio, H8070), Eosin Staining Reagent (Beyotime, C0105-2), Masson Trichrome Staining Reagent (Solarbio, G1340), TRIzol®Reagent (Life, Cat. no. 15596-018), SureScript™ First-Strand cDNA Synthesis Kit (GeneCopoeia, QP056), Immobilon Western HRP Substrate Luminol Reagent (Affinity, KF001), 30% Acrylamide/Bis solution (29:1) (Solarbio, A1010), 24-well plate (VIRYA, 3512409), FBS(Bio-Channel, BC-SE-FBS07), Hieff® Quick exosome isolation kit Plus (YEASON, China).
Tissue embedding kit (Jiangsu Shitai, 20084), Real-timePCR (Thermo Fisher Scientific, TCR0096, USA), UV spectrophotometer (ALLSHENG, USA), benchtop low-speed centrifuge (Weil Ltd., China), inverted fluorescence microscope (Zeiss, Observer.A1, Germany), gel imager (Monad, USA), ice maker (FM150KE, China), 4 °C refrigerator (Haier, China), baking machine (Thermo Scientific, DB-B2, USA), TEM(ZCIBIO, China), NTA(Particle Metrix, China).

SPI (92% protein) for the preparation of peptides was purchased from Shandong Yuxin Bio-Tech Co., Ltd. (Shandong, China). Neutral and alkaline proteases used for enzymolysis of the SPI were provided by Novozymes (Beijing, China). Rainbow pre-stained protein marker (11–180 kDa) was purchased from Genview (Glenview, USA); Laemmli sample buffer was purchased from Bio-Rad (Hercules, USA); Tris-glycine-SDS, 30% Acrylamide/Bis solution, and urea were purchased from Solarbio (Beijing, China); and ammonium persulfate, β-mercaptoethanol, and tetramethylethylenediamine (TEMED) were purchased from Ameresco (Framingham, USA). Pentobarbital Sodium was purchased from Aikon Biomedicine Co., Ltd. (Jiangsu, China). Rat IgM, IgG, IgA, IL-1β, TNF-α, and RANTES ELISA quantification kits were purchased from Myhalic Biotechnology Co., Ltd (Catalog number: RA20444 for IgM, RA20096 for IgG, RA20442 for IgA, RA20020 for IL-1β, RA20035 for TNF-α, RA20511 for RANTES, Bioswamp, Wuhan, China).