Flavan 3 ols

Manufactured by Merck Group

Sourced in United Kingdom, Germany

Flavan-3-ols are a class of organic compounds that are part of the flavonoid family. They are naturally occurring secondary metabolites found in various plant species. Flavan-3-ols function as antioxidants and have been the subject of research in the field of plant biochemistry.

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2 protocols using flavan 3 ols

Lipid Bilayer Characterization with Phytochemicals

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CaCl₂, NaCl, NaOH, calcein, Triton X-100, Sephadex G-50, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), dimethylsulfoxide (DMSO), and plant polyphenols: chalcones (4′-hydroxychalcone (≥99%, high-performance liquid chromatography (HPLC)), cardamonin (≥98%, HPLC), iso liquiritigenin (≥98%, HPLC)), dihydrochalcones (phloretin (≥99%, HPLC)), stilbenes (resveratrol (≥99%, HPLC), piceatannol (≥98%, HPLC)), isoflavones (daidzein (≥98%, HPLC), biochanin A (≥95%, HPLC), genistein (≥98%, HPLC), genistin (≥95%, HPLC)), flavanones (liquiritigenin (≥97%, HPLC), naringenin (≥95%, HPLC)), flavan-3-ols (catechin (≥98%, HPLC)), flavononols (taxifolin (≥90%, HPLC)), flavonols (quercetin, myricetin, and rutin), and lignans (honokiol (≥98%, HPLC)) were purchased from Sigma-Aldrich Company Ltd. (Gillingham, United Kingdom).
Lipids synthetic 1,2-dioleoyl-sn-glycero-3-phospho-(1-rac-glycerol) (DOPG), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phospho-(1-rac-glycerol) (DPPG), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and cholesterol (CHOL) were obtained from Avanti Polar Lipids (Avanti Polar Lipids, Inc., Alabaster, AL, USA).

Zlodeeva P.D., Shekunov E.V., Ostroumova O.S, & Efimova S.S. (2023). The Degree of Hydroxylation of Phenolic Rings Determines the Ability of Flavonoids and Stilbenes to Inhibit Calcium-Mediated Membrane Fusion. Nutrients, 15(5), 1121.

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Comprehensive Analytical Standards Protocol

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Acetonitrile, methanol, formic acid and water (LC-MS grade) were purchased from VWR International (Fontenay-sous-Bois, France). Folin-Ciocalteu's reagent, sodium carbonate and reagents used for biological assays were from Sigma Aldrich (Germany). The commercial standards at disposal were: flavonols (quercetin, quercetin-3-O-rutinoside, quercetin-3-Ogalactoside, quercetin-3-O-glucoside, quercetin-3-Oglucuronide, kaempferol and kaempferol-3-O-glucoside), flavan-3-ols (epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, procyanidin B2), the stilbene resveratrol and trans-piceid; these standard as well as the reactive 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) were from Sigma-Aldrich (Steinem, Germany). Organic acids (caftaric acid, ferulic acid, chlorogenic acid and caffeic acid) and anthocyanins (delphinidin-3-O-glucoside, cyanidin-3-Oglucoside, petunidin-3-O-glucoside, peonidin-3-O-glucoside and malvidin-3-O-glucoside) were from Extrasynthèse (Lyon, France). All standards were stored according to the supplier's instructions and then solubilized at concentration of 1 mg/mL in a water:methanol mixture (1:1, v/v), and diluted to a final concentration of 10 μg/mL and injected in the chromatographic system.

Colombo F ., Di Lorenzo C ., Regazzoni L ., Fumagalli M ., Sangiovanni E ., Peres de Sousa L ., Bavaresco L ., Tomasi D ., Bosso A ., Aldini G ., Restani P , & Dell'Agli M . (2019). Phenolic profiles and anti-inflammatory activities of sixteen table grape (Vitis vinifera L.) varieties. Food & function, 10(4).

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