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Agilent 1260 infinity multi detector gpc sec system

Manufactured by Agilent Technologies
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Sourced in United States

The Agilent 1260 Infinity Multi-Detector GPC/SEC System is a high-performance gel permeation chromatography (GPC) and size exclusion chromatography (SEC) solution. It is designed to provide accurate and reliable analysis of molecular weight and distribution for a wide range of polymer and macromolecular samples.

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Gpc sec mds software, by Agilent Technologies (1 mentions)

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2 protocols using agilent 1260 infinity multi detector gpc sec system

1

Molecular Weight Determination of EPSKar1

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The molecular weight (Mw) of EPSKar1 was determined by size-exclusion chromatography using Agilent 1260 Infinity Multi-Detector GPC/SEC System (Agilent 1260 Infinity Series, USA) with refractive index detector and PL aquagel column (Agilent Technologies). Briefly, 2 mg of EPSKar1 was dissolved in 1 mL distilled water and filtered through a 0.22-μm pore size filter. Data collection and processing were performed using the Agilent GPC/SEC MDS software. The corresponding molecular weight of EPSKar1 was determined using a standard curve of dextran with different molecular weights (5.20, 11.60, 23.80, 48.60, 148.00, 273.00, 410.00, and 668.00 kDa).
Kumari M., Kumari R., Nataraj B.H., Shelke P.A., Ali S.A., Nagpal R, & Behare P.V. (2023). Physicochemical and rheological characterizations of a novel exopolysaccharide EPSKar1 and its iron complex EPSKar1-Fe: Towards potential iron-fortification applications. Current Research in Food Science, 6, 100478.
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2

Copolymer Molecular Weight Analysis

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Weight-average molecular weight ( M¯w ), polydispersity index ( M¯w/M¯n) , of each copolymer was determined by a size exclusion chromatography (SEC) analysis, performed using Tosho Bioscience TSK-Gel G4000 PWXL and G3000 PWXL columns(Sursee, Switzerland) connected to an Agilent 1260 Infinity Multi-Detector GPC/SEC system(Santa Clara, United States), and a refractive index detector. Analyses were performed with buffer citrate/phosphate 0.15 M + 0.1 M NaCl pH 5 as eluent with a flow of 1 mL/min and poly(ethylene oxide) standard (40 kDa) to obtain the calibration curve. The column temperature was set at 30 °C.
Craparo E.F., Drago S.E., Mauro N., Giammona G, & Cavallaro G. (2020). Design of New Polyaspartamide Copolymers for siRNA Delivery in Antiasthmatic Therapy. Pharmaceutics, 12(2), 89.
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